Nature Communications, 2021 The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high
Weronika Witkowska McConnell +11 more
semanticscholar +1 more source
Loop-mediated Isothermal Amplification Reaction Using a Nondenatured Template [PDF]
Clinical Chemistry, 2001 Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions (1). The LAMP method requires a set of four specially designed primers and a DNA polymerase with strand displacement activity.
K, Nagamine +4 more
openaire +2 more sources
Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits [PDF]
, 2020 The success of fundamental and applied nucleic acid (NA) research depends on NA purity, but obtaining pure NAs from raw, unprocessed samples is challenging.
Ismagilov, Rustem F. +2 more
core
JAMA Network Open, 2022 Key Points Question Can loop-mediated isothermal amplification (LAMP)-based methodology coupled with smartphone detection provide an inexpensive, rapid, sensitive, and reliable platform for COVID-19 and influenza testing? Findings In this cohort study of
D. M. Heithoff +11 more
semanticscholar +1 more source
Wolbachia detection in insects through LAMP: loop mediated isothermal amplification
Parasites & Vectors, 2014 Background The bacterium Wolbachia is a promising agent for the biological control of vector-borne diseases as some strains have the ability to block the transmission of key human disease-causing pathogens.
Daniela da Silva Gonçalves +4 more
doaj +1 more source
Development of SARS-CoV-2 Isothermal Amplification Detection Kits
Nano Biomedicine and Engineering, 2020 The SARS-CoV-2 isothermal amplification detection kits based on loop-mediated isothermal amplification (LAMP) were developed and evaluated on three types samples of SARS-CoV-2. The kits included enzyme reaction mixtures and chromogenic agents.
Xueling Li +7 more
doaj +1 more source
Microfluidic method for rapid turbidimetric detection of the DNA of Mycobacterium tuberculosis using loop-mediated isothermal amplification in capillary tubes [PDF]
, 2015 We describe a microfluidic method for rapid isothermal turbidimetric detection of the DNA of Mycobacterium tuberculosis. Loop-mediated isothermal amplification is accomplished in capillary tubes for amplifying DNA in less than 15 min, and sensitivity and
Gill, P., Rafati, A.
core
Advanced Functional Materials, EarlyView.The catalytic valorization of biomass represents an essential approach for achieving sustainable chemical production, with formic acid (FA) being recognized as a valuable platform chemical for hydrogen storage and environmentally friendly synthetic applications.
Yiqi Geng +6 more
wiley +1 more source
Using detergent to enhance detection sensitivity of African trypanosomes in human CSF and blood by Loop-Mediated Isothermal Amplification (LAMP) [PDF]
, 2011 <p><b>Background:</b> The loop-mediated isothermal amplification (LAMP) assay, with its advantages of simplicity, rapidity and cost effectiveness, has evolved as one of the most sensitive and specific methods for the detection of a ...
A Johannessen +54 more
core +10 more sources
A fully integrated paperfluidic molecular diagnostic chip for the extraction, amplification, and detection of nucleic acids from clinical samples [PDF]
, 2016 Paper diagnostics have successfully been employed to detect the presence of antigens or small molecules in clinical samples through immunoassays; however, the detection of many disease targets relies on the much higher sensitivity and specificity ...
Dewar, Rajan +4 more
core +2 more sources