Results 11 to 20 of about 271,314 (298)

The Inducible lac Operator-Repressor System Is Functional in Zebrafish Cells

open access: yesFrontiers in Genetics, 2021
BackgroundZebrafish are a foundational model organism for studying the spatio-temporal activity of genes and their regulatory sequences. A variety of approaches are currently available for editing genes and modifying gene expression in zebrafish ...
Sierra S. Nishizaki   +8 more
doaj   +1 more source

Quantitative immunohistochemistry using an antibody-fused bioluminescent protein

open access: yesBioTechniques, 2020
We established a quantitative detection method for immunohistochemistry based on a reference standard light-emitting diode, protein microarray and antibody-fused bioluminescent protein.
Ke-Yong Wang   +6 more
doaj   +1 more source

Streamlined and Robust Stage-Specific Profiling of Gametocytocidal Compounds Against Plasmodium falciparum

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
Malaria elimination is dependent on the ability to target both the pathogenic and transmissible stages of the human malaria parasite, Plasmodium falciparum.
Janette Reader   +3 more
doaj   +1 more source

Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase

open access: yesSensors, 2018
We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus
Duc Long Nguyen   +5 more
doaj   +1 more source

A High-Content Screen for the Identification of Plant Extracts with Insulin Secretion-Modulating Activity

open access: yesPharmaceuticals, 2021
Bioactive plant compounds and extracts are of special interest for the development of pharmaceuticals. Here, we describe the screening of more than 1100 aqueous plant extracts and synthetic reference compounds for their ability to stimulate or inhibit ...
Roland Hager   +10 more
doaj   +1 more source

Chemical composition and biological activities of the Agaricus mushrooms

open access: yesMongolian Journal of Chemistry, 2014
Two species of Agaricus mushroom grown in Mongolia were analyzed for their element content. Biological activity and chemical components study of Agaricus, grown in the Mongolian flora has been investigated for the first time.
L Munkhgerel   +6 more
doaj   +1 more source

A luminescence-based method to assess antigen presentation and antigen-specific T cell responses for in vitro screening of immunomodulatory checkpoints and therapeutics

open access: yesFrontiers in Immunology, 2023
Investigations into the strength of antigen-specific responses in vitro is becoming increasingly relevant for decision making in early-phase research of novel immunotherapeutic approaches, including adoptive cell but also immune checkpoint inhibitor (ICI)
Jimena Álvarez Freile   +6 more
doaj   +1 more source

A Nitrogen Molecular Sensing System, Comprised of the ALLANTOINASE and UREIDE PERMEASE 1 Genes, Can Be Used to Monitor N Status in Rice

open access: yesFrontiers in Plant Science, 2018
Nitrogen (N) is an essential nutrient for plant growth and development, but its concentration in the soil is often insufficient for optimal crop production.
Dong-Keun Lee   +5 more
doaj   +1 more source

Construction and application of luminescent strain of Pectobacterium carotovorum harboring luxCDABE operon

open access: yes浙江大学学报. 农业与生命科学版, 2021
Pectobacterium carotovorum is one of the top ten plant bacterial pathogens in the world. In order to facilitate the observation of the pathogen infection process at the early stage and in vivo quantification of bacterial growth without the tissue ...
ZHONG Lingkun   +4 more
doaj   +1 more source

An Orthotopic Model of Uveal Melanoma in Zebrafish Embryo: A Novel Platform for Drug Evaluation

open access: yesBiomedicines, 2021
Uveal melanoma is a highly metastatic tumor, representing the most common primary intraocular malignancy in adults. Tumor cell xenografts in zebrafish embryos may provide the opportunity to study in vivo different aspects of the neoplastic disease and ...
Chiara Tobia   +7 more
doaj   +1 more source

Home - About - Disclaimer - Privacy