Results 11 to 20 of about 17,864 (198)

Triggering cell death in cancers using self-illuminating nanocomposites

open access: yesFrontiers in Chemistry, 2022
Bioinspired photocatalysis has resulted in efficient solutions for many areas of science and technology spanning from solar cells to medicine. Here we show a new bioinspired semiconductor nanocomposite (nanoTiO2-DOPA-luciferase, TiDoL) capable of ...
Tijana Rajh   +6 more
doaj   +1 more source

Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions

open access: yeseLife, 2019
Technologies that convert transient protein-protein interactions (PPIs) into stable expression of a reporter gene are useful for genetic selections, high-throughput screening, and multiplexing with omics technologies. We previously reported SPARK (Kim et
Christina K Kim   +3 more
doaj   +1 more source

Bioluminescence-Driven Optogenetics

open access: yesLife, 2020
Bioluminescence-based technologies are among the most commonly used methods to quantify and visualise physiology at the cellular and organismal levels.
Macià Sureda-Vives, Karen S. Sarkisyan
doaj   +1 more source

The Inducible lac Operator-Repressor System Is Functional in Zebrafish Cells

open access: yesFrontiers in Genetics, 2021
BackgroundZebrafish are a foundational model organism for studying the spatio-temporal activity of genes and their regulatory sequences. A variety of approaches are currently available for editing genes and modifying gene expression in zebrafish ...
Sierra S. Nishizaki   +8 more
doaj   +1 more source

Quantitative immunohistochemistry using an antibody-fused bioluminescent protein

open access: yesBioTechniques, 2020
We established a quantitative detection method for immunohistochemistry based on a reference standard light-emitting diode, protein microarray and antibody-fused bioluminescent protein.
Ke-Yong Wang   +6 more
doaj   +1 more source

Construction and application of luminescent strain of Pectobacterium carotovorum harboring luxCDABE operon

open access: yes浙江大学学报. 农业与生命科学版, 2021
Pectobacterium carotovorum is one of the top ten plant bacterial pathogens in the world. In order to facilitate the observation of the pathogen infection process at the early stage and in vivo quantification of bacterial growth without the tissue ...
ZHONG Lingkun   +4 more
doaj   +1 more source

Effect of Prooxidative Natural Products: Comparison of the OSI1 (YKL071w) Promoter Luciferase Construct from Yeast with an Nrf2/Keap Reporter System

open access: yesApplied Sciences, 2020
The oxidative stress response (OSR) in yeast is under the control of oxidation-sensitive cysteines in the Yap1p transcription factor, and fusion of the Yap1p-dependent OS-induced promoter of the YKL071w gene (OSI1) to a luciferase coding sequence makes a
Ivan Schlembach   +5 more
doaj   +1 more source

Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase

open access: yesSensors, 2018
We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus
Duc Long Nguyen   +5 more
doaj   +1 more source

Luciferase‐Induced Photouncaging: Bioluminolysis

open access: yesAngewandte Chemie International Edition, 2019
Abstract Bioluminescence resonance energy transfer (BRET) has been widely used for studying dynamic processes in biological systems such as protein–protein interactions and other signaling events. Aside from acting as a reporter, BRET can also turn on functions in living systems.
Dalu Chang   +5 more
openaire   +3 more sources

Chemical composition and biological activities of the Agaricus mushrooms

open access: yesMongolian Journal of Chemistry, 2014
Two species of Agaricus mushroom grown in Mongolia were analyzed for their element content. Biological activity and chemical components study of Agaricus, grown in the Mongolian flora has been investigated for the first time.
L Munkhgerel   +6 more
doaj   +1 more source

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