Results 21 to 30 of about 17,864 (198)

IgE-Crosslinking-Induced Luciferase Expression Test as a Sensitive Indicator of Anisakis Allergy

Antibodies
Background: Anisakis allergy has been increasing, and the diagnosis of it is based on specific serum IgE detection. Recently, the IgE-crosslinking-induced luciferase expression (EXiLE) test has been proposed as convenient tool for detecting functionally ...
Haruyo Akiyama, Masashi Niwa, Chisato Kurisaka, Yuto Hamada, Yuma Fukutomi, Reiko Teshima   +5 more
doaj   +1 more source

In vitro–In vivo Hybrid Approach for Studying Modulation of NRF2 in Immortalized Bovine Mammary Cells

Frontiers in Animal Science, 2021
Nuclear factor erythroid 2-related factor 2 (NRF2) plays a key role in the response to oxidative stress. Diets containing known NRF2 modulators could be used to minimize oxidative stress in dairy cows.
Hunter R. Ford, Sebastiano Busato, Massimo Bionaz   +2 more
doaj   +1 more source

Bioluminescent Enzyme Inhibition Based Assay of Metal Nanoparticles [PDF]

Журнал Сибирского федерального университета: Серия Биология, 2017
The bioluminescent enzymatic bioassays for assessment of nanomaterial biotoxicity using the soluble or immobilized coupled enzyme system of luminous bacteria NAD(P)Н:FMN-oxidoreductase + luciferase (Red + Luc) as a test system were employed in this study.
Yana V. Fritsler, Elena N. Esimbekova, Valentina A. Kratasyuk   +2 more
doaj  

Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells

Frontiers in Bioengineering and Biotechnology, 2021
Technologies enabling on-site environmental detection or medical diagnostics in resource-limited settings have a strong disruptive potential compared to current analytical approaches that require trained personnel in laboratories with immobile, resource ...
Mark Wienhold, Andrew Kirkpatrick, Tingting Xu, Steven Ripp, Steven Ripp, Gary Sayler, Dan Close   +6 more
doaj   +1 more source

Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with Firefly Luciferase [PDF]

Molecular Imaging, 2011
Gaussia luciferase (Gluc) is a secreted reporter, and its expression in living animals can be assessed by in vivo bioluminescence imaging (BLI) or blood assays. We characterized Gluc as an in vivo reporter in comparison with firefly luciferase (Fluc). Mice were inoculated subcutaneously with tumor cells expressing both Fluc and Gluc and underwent Flue
Yusuke Inoue, Fugeng Sheng, Shigeru Kiryu, Makoto Watanabe, Harnprasopwat Ratanakanit, Kiyoko Izawa, Arinobu Tojo, Kuni Ohtomo   +7 more
openaire   +3 more sources

A Nitrogen Molecular Sensing System, Comprised of the ALLANTOINASE and UREIDE PERMEASE 1 Genes, Can Be Used to Monitor N Status in Rice

Frontiers in Plant Science, 2018
Nitrogen (N) is an essential nutrient for plant growth and development, but its concentration in the soil is often insufficient for optimal crop production.
Dong-Keun Lee, Mark C. F. R. Redillas, Harin Jung, Seowon Choi, Youn Shic Kim, Ju-Kon Kim   +5 more
doaj   +1 more source

Screening of Fungi for Antimycobacterial Activity Using a Medium-Throughput Bioluminescence-Based Assay

Frontiers in Microbiology, 2021
There is a real and urgent need for new antibiotics able to kill Mycobacteria, acid-fast bacilli capable of causing multiple deadly diseases. These include members of the Mycobacterium tuberculosis complex, which causes the lung disease tuberculosis (TB)
Alexander B. J. Grey, Melissa M. Cadelis, Melissa M. Cadelis, Yiwei Diao, Duckchul Park, Thomas Lumley, Bevan S. Weir, Brent R. Copp, Siouxsie Wiles   +8 more
doaj   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source