Results 31 to 40 of about 19,699 (283)

Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes [PDF]

, 2013
Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability ...
Badr, Christian E, Bovenberg, M Sarah, Crommentuijn, Matheus HW, Kerami, Mariam, Maguire, Casey A, Niers, Johanna M, Sena-Esteves, Miguel, Tannous, Bakhos A, Teng, Jian   +8 more
core   +2 more sources

A multifunctional, synthetic Gaussia princeps luciferase reporter for live imaging of Candida albicans infections [PDF]

, 2009
Peer reviewedPublisher ...
Brown, Alistair J P, d'Enfert, Christophe, Enjalbert, Brice, Pietrella, Donatella, Rachini, Anna, Spaccapelo, Roberta, Vecchiarelli, Anna, Vediyappan, Govindsamy   +7 more
core   +3 more sources

Development of NanoLuc-PEST expressing Leishmania mexicana as a new drug discovery tool for axenic- and intramacrophage-based assays [PDF]

, 2018
The protozoan parasite Leishmania causes leishmaniasis; a spectrum of diseases of which there are an estimated 1 million new cases each year. Current treatments are toxic, expensive, difficult to administer, and resistance to them is emerging.
Berry, SL, Hameed, H, Horrocks, P, Maciej-Hulme, ML, Price, HP, Saif Abou-Akkada, S, Thomason, A   +6 more
core   +7 more sources

Functional interaction between the ZO-1-interacting transcription factor ZONAB/DbpA and the RNA processing factor symplekin [PDF]

, 2006
Epithelial tight junctions participate in the regulation of gene expression by controlling the activity of transcription factors that can interact with junctional components.
Balda, MS, Buchert, M, Choquet, A, Hollande, F, Kavanagh, E, Matter, K, Tsapara, A   +6 more
core   +1 more source

Multicolor luciferase assay system: one-step monitoring of multiple gene expressions with a single substrate

BioTechniques, 2005
Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression.
Yoshihiro Nakajima, Takuma Kimura, Kazunori Sugata, Toshiteru Enomoto, Atsushi Asakawa, Hidehiro Kubota, Masaaki Ikeda, Yoshihiro Ohmiya   +7 more
doaj   +1 more source

A novel-type luciferin from Siberian luminous earthworm Fridericia heliota : structure elucidation by spectral studies and total synthesis [PDF]

, 2014
Author Posting. © The Author(s), 2014. This is the author's version of the work. It is posted here by permission of Wiley-VCH Verlag GmbH & Co for personal use, not for redistribution.
Baranov, Mikhail S., Dubinnyi, Maxim A., Kublitski, Vadim S., Petushkov, Valentin N., Rodionova, Natalja S., Shimomura, Osamu, Tsarkova, Aleksandra S., Yampolsky, Ilia V.   +7 more
core   +1 more source

Reporter gene comparison demonstrates interference of complex body fluids with secreted luciferase activity

Scientific Reports, 2021
Reporter gene assays are widely used to study cellular signaling and transcriptional activity. Few studies describe the use of reporter genes for studying cellular responses on complex body fluids, such as urine and blood.
M. Neefjes, B. A. C. Housmans, G. G. H. van den Akker, L. W. van Rhijn, T. J. M. Welting, P. M. van der Kraan   +5 more
doaj   +1 more source

Photophore Distribution and Enzymatic Diversity Within the Photogenic Integument of the Cookie-Cutter Shark Isistius brasiliensis (Chondrichthyes: Dalatiidae)

Frontiers in Marine Science, 2021
The cookie-cutter shark Isistius brasiliensis (Squaliformes: Dalatiidae) is a deep-sea species that emits a blue luminescence ventrally, except at the level of a black band located beneath the jaw.
Jérôme Delroisse, Laurent Duchatelet, Patrick Flammang, Jérôme Mallefet   +3 more
doaj   +1 more source

Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy. [PDF]

PLoS ONE, 2018
Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most
JingZhe Li, ZhiLan Guo, Takashi Sato, Bo Yuan, YanYan Ma, Dan Qian, JuYing Zhong, MengMeng Jin, Peng Huang, LuYang Che, Yi Wang, Yan Lei, ChangZhen Liu   +12 more
doaj   +1 more source

Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging

iScience, 2021
Summary: For in vivo multicolor bioluminescence applications, red and near-infrared signals are desirable over shorter wavelength signals because they are not as susceptible to light attenuation by blood and tissue. Herein, we describe the development of
Giorgia Zambito, Mary P. Hall, Monika G. Wood, Natasa Gaspar, Yanto Ridwan, Fabio F. Stellari, Ce Shi, Thomas A. Kirkland, Lance P. Encell, Clemens Löwik, Laura Mezzanotte   +10 more
doaj   +1 more source