Sensitive dual color in vivo bioluminescence imaging using a new red codon optimized firefly luciferase and a green click beetle luciferase. [PDF]
PLoS ONE, 2011 BACKGROUND: Despite a plethora of bioluminescent reporter genes being cloned and used for cell assays and molecular imaging purposes, the simultaneous monitoring of multiple events in small animals is still challenging. This is partly attributable to the
Laura Mezzanotte +5 more
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A higher spectral range of beetle bioluminescence with infraluciferin
Frontiers in Bioengineering and Biotechnology, 2022 Coleopteran bioluminescence is unique in that beetle luciferases emit colors ranging between green (ca.550 nm) and red (ca.600 nm), including intermediate colors such as yellow and orange, allowing up to 3 simultaneous parameters to be resolved in vitro ...
Amit P. Jathoul +4 more
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Development of NanoLuc-PEST expressing Leishmania mexicana as a new drug discovery tool for axenic- and intramacrophage-based assays [PDF]
, 2018 The protozoan parasite Leishmania causes leishmaniasis; a spectrum of diseases of which there are an estimated 1 million new cases each year. Current treatments are toxic, expensive, difficult to administer, and resistance to them is emerging.
Berry, SL +6 more
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A panel of Trypanosoma brucei strains tagged with blue and red-shifted luciferases for bioluminescent imaging in murine infection models. [PDF]
PLoS Neglected Tropical Diseases, 2014 BACKGROUND:Genetic engineering with luciferase reporter genes allows monitoring Trypanosoma brucei (T.b.) infections in mice by in vivo bioluminescence imaging (BLI). Until recently, luminescent T.b.
Nick Van Reet +4 more
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A novel-type luciferin from Siberian luminous earthworm Fridericia heliota : structure elucidation by spectral studies and total synthesis [PDF]
, 2014 Author Posting. © The Author(s), 2014. This is the author's version of the work. It is posted here by permission of Wiley-VCH Verlag GmbH & Co for personal use, not for redistribution.
Baranov, Mikhail S. +7 more
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Luciferase‐Induced Photouncaging: Bioluminolysis
Angewandte Chemie International Edition, 2019 Abstract Bioluminescence resonance energy transfer (BRET) has been widely used for studying dynamic processes in biological systems such as protein–protein interactions and other signaling events. Aside from acting as a reporter, BRET can also turn on functions in living systems.
Dalu Chang +5 more
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Luciferase-Rose Bengal Conjugates for Single Oxygen Generation by Bioluminescence Resonant Energy Transfer [PDF]
, 2019 Conjugates of Rose Bengal and Renilla luciferase generated singlet oxygen upon binding with coelenterazine via bioluminescence resonance energy transfer (BRET).
Choi, Myung-Gyu +5 more
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BioTechniques, 2005 Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression.
Yoshihiro Nakajima +7 more
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Ricin trafficking in plant and mammalian cells [PDF]
, 2011 Ricin is a heterodimeric plant protein that is potently toxic to mammalian and many other eukaryotic cells. It is synthesized and stored in the endosperm cells of maturing Ricinus communis seeds (castor beans).
Lord, Mike, Spooner, Robert A.
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Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging [PDF]
, 2018 The sensitivity of bioluminescence imaging in animals is primarily dependent on the amount of photons emitted by the luciferase enzyme at wavelengths greater than 620 nm where tissue penetration is high.
Encell, L.P. (Lance P.) +11 more
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