Results 41 to 50 of about 14,115 (284)
Scientific Reports, 2021 Reporter gene assays are widely used to study cellular signaling and transcriptional activity. Few studies describe the use of reporter genes for studying cellular responses on complex body fluids, such as urine and blood.
M. Neefjes +5 more
doaj +1 more source
Frontiers in Marine Science, 2021 The cookie-cutter shark Isistius brasiliensis (Squaliformes: Dalatiidae) is a deep-sea species that emits a blue luminescence ventrally, except at the level of a black band located beneath the jaw.
Jérôme Delroisse +3 more
doaj +1 more source
Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with Firefly Luciferase [PDF]
Molecular Imaging, 2011 Gaussia luciferase (Gluc) is a secreted reporter, and its expression in living animals can be assessed by in vivo bioluminescence imaging (BLI) or blood assays. We characterized Gluc as an in vivo reporter in comparison with firefly luciferase (Fluc). Mice were inoculated subcutaneously with tumor cells expressing both Fluc and Gluc and underwent Flue
Yusuke Inoue +7 more
openaire +3 more sources
Chemical communication between synthetic and natural cells: a possible experimental design [PDF]
, 2013 The bottom-up construction of synthetic cells is one of the most intriguing and interesting research arenas in synthetic biology. Synthetic cells are built by encapsulating biomolecules inside lipid vesicles (liposomes), allowing the synthesis of one or ...
D'Angelo, Francesca +5 more
core +3 more sources
Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy. [PDF]
PLoS ONE, 2018 Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most
JingZhe Li +12 more
doaj +1 more source
iScience, 2021 Summary: For in vivo multicolor bioluminescence applications, red and near-infrared signals are desirable over shorter wavelength signals because they are not as susceptible to light attenuation by blood and tissue. Herein, we describe the development of
Giorgia Zambito +10 more
doaj +1 more source
Designing lentiviral gene vectors [PDF]
, 2011 Lentiviral gene vectors are an important tool in gene therapy and basic biomedical research. They are transducing viral particles, normally replication defective, which are generated using the packaging machinery of lentiviruses.
Al-Allaf, FA +2 more
core +1 more source
Macromolecular assembly of bioluminescent protein nanoparticles for enhanced imaging
Materials Today Bio, 2022 Bioluminescence imaging has advantages over fluorescence imaging, such as minimal photobleaching and autofluorescence, and greater signal-to-noise ratios in many complex environments.
Enya Li +9 more
doaj +1 more source
Clinical value of luciferase-based bioluminescence assay in diagnosis of Alport syndrome
Frontiers in PediatricsObjectivesAlport syndrome (AS) is an inherited kidney disorder caused by pathogenic variants in COL4A3, COL4A4, or COL4A5. In this study, we aim to apply a split-luciferase bioluminescence assay to functionally assess COL4A3, COL4A4, or COL4A5 variants ...
Yue Cai +6 more
doaj +1 more source
Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing
FEBS Letters, EarlyView.Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley +1 more source