Chemical communication between synthetic and natural cells: a possible experimental design [PDF]
, 2013 The bottom-up construction of synthetic cells is one of the most intriguing and interesting research arenas in synthetic biology. Synthetic cells are built by encapsulating biomolecules inside lipid vesicles (liposomes), allowing the synthesis of one or ...
D'Angelo, Francesca +5 more
core +3 more sources
Macromolecular assembly of bioluminescent protein nanoparticles for enhanced imaging
Materials Today Bio, 2022 Bioluminescence imaging has advantages over fluorescence imaging, such as minimal photobleaching and autofluorescence, and greater signal-to-noise ratios in many complex environments.
Enya Li +9 more
doaj +1 more source
Designing lentiviral gene vectors [PDF]
, 2011 Lentiviral gene vectors are an important tool in gene therapy and basic biomedical research. They are transducing viral particles, normally replication defective, which are generated using the packaging machinery of lentiviruses.
Al-Allaf, FA +2 more
core +1 more source
Codon-Optimized Luciferase Variants for Mammalian Gene Expression in Culture and in Vivo
Molecular Imaging, 2012 Luciferases have proven to be useful tools in advancing our understanding of biologic processes. Having a multitude of bioluminescent reporters with different properties is highly desirable.
Casey A. Maguire +4 more
doaj +1 more source
Luciferase-Rose Bengal Conjugates for Single Oxygen Generation by Bioluminescence Resonant Energy Transfer [PDF]
, 2019 Conjugates of Rose Bengal and Renilla luciferase generated singlet oxygen upon binding with coelenterazine via bioluminescence resonance energy transfer (BRET).
Choi, Myung-Gyu +5 more
core +1 more source
Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing
FEBS Letters, EarlyView.Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley +1 more source
Functional interaction of STAT3 transcription factor with the coactivator NcoA/SRC1a. [PDF]
, 2002 Signal transducer and activator of transcription 3 (STAT3) transcription factors are cytoplasmic proteins that induce gene activation in response to cytokine receptor stimulation.
D.M. Heery +5 more
core +2 more sources
Function‐driven design of a surrogate interleukin‐2 receptor ligand
FEBS Letters, EarlyView.Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang +9 more
wiley +1 more source
Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence
Molecular Imaging, 2004 Luciferase from the North American firefly ( Photinis pyralis ) is a useful reporter gene in vivo, allowing noninvasive imaging of tumor growth, metastasis, gene transfer, drug treatment, and gene expression.
Brenda Baggett +6 more
doaj +1 more source
Improved Red-emitting Firefly Luciferase Mutant for Biotechnical Applications [PDF]
, 2009 Luciferase from the North American firefly Photinus Pyralis catalyzes a highly efficient bioluminescent reaction that produces yellow-green light (557 nm at pH 7.8).
Davis, Audrey L
core +1 more source