Results 31 to 40 of about 45,749 (264)

5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems.

PLoS ONE, 2020
Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings.
Ce Shi, Michael P Killoran, Mary P Hall, Paul Otto, Monika G Wood, Ethan Strauss, Lance P Encell, Thomas Machleidt, Keith V Wood, Thomas A Kirkland   +9 more
doaj   +1 more source

Successful delivery of viviparous lantern shark from an artificial uterus and the self-production of lantern shark luciferin.

PLoS ONE, 2023
Our recent success in the long-term maintenance of lantern shark embryos in artificial uterine systems has provided a novel option for the medical treatment of premature embryos for captive viviparous elasmobranchs.
Taketeru Tomita, Minoru Toda, Atsushi Kaneko, Kiyomi Murakumo, Kei Miyamoto, Keiichi Sato   +5 more
doaj   +1 more source

Immobilization of Firefly Bioluminescent System: Development and Application of Reagents

Biosensors, 2022
The present study describes the method of preparing reagents containing firefly luciferase (FLuc) and its substrate, D-luciferin, immobilized into gelatin gel separately or together.
Elena N. Esimbekova, Maria A. Kirillova, Valentina A. Kratasyuk   +2 more
doaj   +1 more source

Myogenic Fusogen‐Engineered Lipid Nanoparticles Enhance mRNA Delivery in Skeletal Muscle

Advanced Functional Materials, EarlyView.
This study reports a biomimetic strategy of engineering full‐length Myomaker, a muscle‐specific fusogen, into lipid nanoparticles (LNPs) to harness the native myoblast fusion capability for skeletal muscle mRNA delivery. The resulting Mymk‐LNPs enhance transfection in differentiating myocytes and enable Cre‐mediated reporter activation in injured ...
Fangyu Zhang, Zhisheng Xu, Yaou Duan, Yehui Sun, Shiying Wu, Tao Long, Mateusz Durbacz, Li Zhang, Yufen Xiao, Yu‐Chung Pien, Erick D. Guerrero, Zeru Tian, Xu Wang, Pratima Basak, Sumanta Chatterjee, Ning Liu, Xiaochun Li, Eric N. Olson, Daniel J. Siegwart   +18 more
wiley   +1 more source

Development of a cell-based bioassay for phospholipase A2-triggered liposomal drug release. [PDF]

PLoS ONE, 2015
The feasibility of exploiting secretory phospholipase A2 (sPLA2) enzymes, which are overexpressed in tumors, to activate drug release from liposomes precisely at the tumor site has been demonstrated before.
Ahmad Arouri, Jakub Trojnar, Steffen Schmidt, Anders H Hansen, Jan Mollenhauer, Ole G Mouritsen   +5 more
doaj   +1 more source

A Holistic Platform Approach for Developing Robust Pulmonary mRNA Delivery: Integrating Novel Phenolic Acid‐Derived Ionizable Lipids and Device Engineering

Advanced Functional Materials, EarlyView.
An integrated pulmonary mRNA delivery platform combining novel biodegradable syringic acid‐derived ionizable lipids, design‐of‐experiments formulation optimization, and vibrating‐mesh nebulizer engineering enabled stable aerosolization and efficient lung delivery.
Neha Kaushal, Younan Ma, Asad Khanmohammed, Dipen Parande, Hanming Zhang, Saket Dolare, Juliet Chen, Apiwat Wangweerawong, Saswata Karmakar, Hongfeng Deng, Frank Koppenhagen, Shraddha Sharma, Eric Reyes, Sherry He, Lianne Boeglin, Caroline Woo, Azadeh Paksa, Ayed Allawzi, Anusha Dias, Gregory Troiano, Shrirang Karve, Frank DeRosa   +21 more
wiley   +1 more source

Esterified Cholesterol Conjugates Enable LNP‐Mediated mRNA Delivery to the Blood–Brain Barrier Following Intravenous Administration

Advanced Materials, EarlyView.
A cholesterol‐conjugated lipid library enabled the identification of a ligand‐free LNP platform for efficient mRNA delivery to brain endothelial cells via systemic administration. This platform achieves selective BBB targeting without disrupting barrier integrity, and enables modulation of neuroinflammation and vascular function without requiring trans‐
Zeru Tian, Xu Wang, Alexis Pacheco Benitez, Erick Guerrero, Yaou Duan, Jingwen Wei, Lukas Farbiak, Daniel J. Siegwart   +7 more
wiley   +1 more source

Novel in vivo imaging analysis of an inner ear drug delivery system in mice: comparison of inner ear drug concentrations over time after transtympanic and systemic injections. [PDF]

PLoS ONE, 2012
OBJECTIVE: Systemic steroid injections are used to treat idiopathic sudden-onset sensorineural hearing loss (ISSHL) and some inner ear disorders. Recent studies show that transtympanic (TT) steroid injections are effective for treating ISSHL.
Sho Kanzaki, Masato Fujioka, Akimasa Yasuda, Shinsuke Shibata, Masaya Nakamura, Hirotaka James Okano, Kaoru Ogawa, Hideyuki Okano   +7 more
doaj   +1 more source

Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency

Advanced Materials, EarlyView.
As lipid nanoparticle (LNP) systems are the leading platform for in vivo nucleic acid delivery, improving transfection potency of these systems is of considerable interest. In this work, we explored a post‐mixing formulation method involving a two‐step buffer exchange that increased the number of mRNA copies within each LNP while reducing the number of
Yao Zhang, Jerry Leung, Judy Cheng, Arash Momeni, Albert Kamanzi, Ariadne Tuckmantel Bido, Suiyang Liao, Emma Kang, Colton Strong, Christian J. Kastrup, Sabrina Leslie, Miffy Hok Yan Cheng, Pieter R. Cullis   +12 more
wiley   +1 more source

UDP‐Glucose‐6‐Dehydrogenase Mediated O‐GlcNAcylation of Tight Junction Protein 1 Suppresses Metastasis in Renal Cell Carcinoma

Advanced Science, EarlyView.
Our research unveiled a regulatory paradigm wherein TRIM25 orchestrates the ubiquitin‐mediated degradation of UGDH. UGDH modulates the protein stability of TJP1 by regulating O‐GlcNAcylation levels, effectively impeding the metastasis of ccRCC. Our insights elevate UGDH to a pivotal biomarker and tumor suppressor, marking the first demonstration that ...
Xiaolin Chen, Yuhang Bao, Tao Liu, Jianhui Qiu, Yuanyuan Ma, Zedan Zhang, Chuandong Wang, Ruiyi Deng, Zheng Song, Tianyi He, Haode Liu, Jingcheng Zhou, Yizhou Wang, Kan Gong   +13 more
wiley   +1 more source