Results 51 to 60 of about 67,421 (349)
Optogenetic inhibition of neurons by internal light production
Frontiers in Behavioral Neuroscience, 2014 Optogenetics is an extremely powerful tool for selective neuronal activation/inhibition and dissection of neural circuits. However, a limitation of in vivo optogenetics is that an animal must be tethered to an optical fiber for delivery of light.
Benjamin eLand +4 more
doaj +1 more source
5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems.
PLoS ONE, 2020 Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings.
Ce Shi +9 more
doaj +1 more source
Immobilization of Firefly Bioluminescent System: Development and Application of Reagents
Biosensors, 2022 The present study describes the method of preparing reagents containing firefly luciferase (FLuc) and its substrate, D-luciferin, immobilized into gelatin gel separately or together.
Elena N. Esimbekova +2 more
doaj +1 more source
Scientific Reports, 2019 How the unique luciferase of Phrixothrix hirtus (PxRE) railroad worm catalyzes the emission of red bioluminescence using the same luciferin of fireflies, remains a mystery. Although PxRE luciferase is a very attractive tool for bioanalysis and bioimaging
V. R. Bevilaqua +5 more
semanticscholar +1 more source
Advanced Healthcare Materials, EarlyView.Organelle localization‐induced biorthogonal polymerization enables direct synthesis of photostable poly‐AIEgens within targeted organelles for super‐resolution live‐cell imaging. ABSTRACT Real‐time monitoring of dynamic biological processes demands fluorescent probes that can withstand prolonged light exposure without photobleaching—a critical ...
Gaeun Park +4 more
wiley +1 more source
Development of a cell-based bioassay for phospholipase A2-triggered liposomal drug release. [PDF]
PLoS ONE, 2015 The feasibility of exploiting secretory phospholipase A2 (sPLA2) enzymes, which are overexpressed in tumors, to activate drug release from liposomes precisely at the tumor site has been demonstrated before.
Ahmad Arouri +5 more
doaj +1 more source
Multicomponent Bioluminescence Imaging with Naphthylamino Luciferins
ChemBioChem, 2021 AbstractBioluminescent tools have been used for decades to image processes in complex tissues and preclinical models. However, few distinct probes are available to probe multicellular interactions. We and others are addressing this limitation by engineering new luciferases that can selectively process synthetic luciferin analogues.
Caroline K. Brennan +3 more
openaire +4 more sources
Advanced Healthcare Materials, EarlyView.This study shows that stem‐like cells isolated from breast cancer brain metastases drive the spread and growth of tumors in the brain. Among them, a highly adhesive subgroup initiates metastasis and resists many drugs. By closely reflecting patient tumors, these models help identify new targets and improve the development of effective treatments ...
Stefania Faletti +27 more
wiley +1 more source
The HSV-1 Latency-Associated Transcript Functions to Repress Latent Phase Lytic Gene Expression and Suppress Virus Reactivation from Latently Infected Neurons [PDF]
, 2016 open access articleHerpes simplex virus 1 (HSV-1) establishes life-long latent infection within sensory neurons, during which viral lytic gene expression is silenced.
Coleman, Heather M. +7 more
core +3 more sources
CORE: Cholesterol Altered Lipid Nanoparticles for Splenic Expression of mRNA Payloads
Advanced Healthcare Materials, EarlyView.In this paper researchers introduce CORE LNPs, a new class of lipid nanoparticles engineered to redirect mRNA expression away from the liver and into the spleen, a key immune organ. By combining chemical design with computational tools, they created cholesterol analogs that enable precise spleen‐targeted expression, providing greater applications for ...
Eshan A. Narasipura +4 more
wiley +1 more source