Results 51 to 60 of about 44,149 (263)
Enhanced Intracellular Stability and Translation Efficiency of mRNA Drugs by a 2‐arm mRNA Platform
Advanced Science, EarlyView.We constructed a 2‐arm mRNA, characterized by a unique topology formed through the dimerization of two mRNA 3’ tails. The 2‐arm mRNA improves 3’ tail stability and resistance to nuclease degradation, resulting in an intracellular half‐life of up to 65 h. This method substantially enhances the translation capacity of mRNA drugs.
Xucong Teng +5 more
wiley +1 more source
Luminescence of Higher Mushrooms [PDF]
Журнал Сибирского федерального университета: Серия Биология, 2012 The review highlights the results of experimental studies on fungal luminescence carried out during last two centuries. Present concepts on the luminescent system and light emission mechanism in higher fungi are discussed.
Vladimir S. Bondar +2 more
doaj
Bioluminescência de fungos: distribuição, função e mecanismo de emissão de luz
Química Nova, 2013 The emission of light by living organisms, bioluminescence, has been studied since the nineteenth century. However, some bioluminescent systems, such as fungi, remain poorly understood.
Anderson Garbuglio Oliveira +3 more
doaj +1 more source
Substrate cooperativity in marine luciferases. [PDF]
PLoS ONE, 2012 Marine luciferases are increasingly used as reporters to study gene regulation. These luciferases have utility in bioluminescent assay development, although little has been reported on their catalytic properties in response to substrate concentration ...
George Tzertzinis +2 more
doaj +1 more source
Discovery of a Potent Fluorescence Polarization Probe for Identifying USP1 Allosteric Inhibitors
Advanced Science, EarlyView.This study presents the first ubiquitin‐specific protease 1 (USP1) allosteric fluoroprobe and fluorescence polarization assay, enabling the differentiation of allosteric and catalytic site inhibitors. Further, a novel class of tetrahydroisoquinoline‐based USP1 inhibitors is designed, with compound 14a (USP1 IC50 = 29.9 nM) showing strong selectivity ...
Jiawei Cheng +12 more
wiley +1 more source
ATP MONITORING AS AN EXPRESS METHOD TO DETERMINE OBJECT CONTAMINATION
Harčova Nauka ì Tehnologìâ, 2019 The article presents the results of indication of water and surface contamination. The researches were aimed at estimation of water safety and sanitary-hygienic condition of surfaces.
T. Volovyk +5 more
doaj +1 more source
Using bioluminescence to image gene expression and spontaneous behavior in freely moving mice.
PLoS ONE, 2023 Bioluminescence imaging (BLI) of gene expression in live animals is a powerful method for monitoring development, tumor growth, infections, healing, and other progressive, long-term biological processes. BLI remains an effective approach for reducing the
Astha Malik +2 more
doaj +1 more source
Advanced Science, EarlyView.A catalytic allosteric DNAzyme assay (SMART) is developed by engineering DNAzyme into a unimolecular biosensor, enabling highly sensitive and multiplex detection of small molecules and nucleic acids. SMART enables extraction‐free, one‐pot, one‐step, isothermal, and cost‐effective detection of biological markers, achieving >95% accuracy for rapid ...
Yanzhe Shen +12 more
wiley +1 more source
BMC Biology, 2020 Background Luminescent reporter proteins are vital tools for visualizing cells and cellular activity. Among the current toolbox of bioluminescent systems, only bacterial luciferase has genetically defined luciferase and luciferin synthesis pathways that ...
Michael Conway +5 more
doaj +1 more source
Advanced Science, EarlyView.Biological rhythms coordinate physiology, from genes to behavior. Study of circadian rhythms in brain tissue is constrained by limited throughput and spatial and temporal information quality. A new platform for high‐throughput, long‐term multiplexed fluorescent live imaging of circadian rhythms in brain slices is introduced.
Marco Ferrari +3 more
wiley +1 more source