Results 41 to 50 of about 45,749 (264)

In Situ Genetic Engineering Facilitates MMP‐9‐Responsive TIMP1 Release Through Proteolytic Cleavage to Boost Diabetic Wound Healing

Advanced Science, EarlyView.
This study develops an MMP‐9‐responsive protein release system that transforms resident cells into drug depots through in situ genetic engineering. Anchored to the membrane, the therapeutic protein TIMP1 is selectively released upon MMP‐9 cleavage.
Yuan Zhang, Jiani Deng, Man Yee Cheung, Tingting Fu, Xufeng Qi, Dongqing Cai, Jianmin Sun, Gang Lu, Peng Shi, Wai‐Yee Chan, Xu Li, Hui Zhao   +11 more
wiley   +1 more source

A practical, biomimetic, one-pot synthesis of firefly luciferin

Scientific Reports
The bioluminescence reaction of firefly luciferase with D-luciferin has become an indispensable imaging technique in modern biology and life science experiments, but the high cost of D-luciferin is limiting its further application.
Maria Kato, Kazuaki Tsuchihashi, Shusei Kanie, Yuichi Oba, Toshio Nishikawa   +4 more
doaj   +1 more source

Express ELISA for detection of mortalin

BioTechniques, 2019
Mortalin is a widely studied stress chaperone that plays a significant role in diseases such as cancer, diabetes mellitus, liver cirrhosis, neurodegeneration and generalized aging. Based on these, the level of mortalin expression has been predicted to be
Sukant Garg, Chun Wu, Yoshihiro Ohmiya, Sunil C Kaul, Renu Wadhwa   +4 more
doaj   +1 more source

Mathematical Model of the Firefly Luciferase Complementation Assay Reveals a Non-Linear Relationship between the Detected Luminescence and the Affinity of the Protein Pair Being Analyzed. [PDF]

PLoS ONE, 2016
The firefly luciferase complementation assay is widely used as a bioluminescent reporter technology to detect protein-protein interactions in vitro, in cellulo, and in vivo.
Renee Dale, Yuki Ohmuro-Matsuyama, Hiroshi Ueda, Naohiro Kato   +3 more
doaj   +1 more source

TET1 Inhibition Promotes Therapeutic Sensitivity in TP53‐Mutant GBM by Influencing Genome Fragility and Altering TAMs Biology

Advanced Science, EarlyView.
In TP53mut GBM cells, reduced P53 function is associated with increased TET1 expression. Genetic or pharmacological inhibition of TET1 correlates with genome fragility, including DNA damage, cellular senescence, telomere shortening, and reactive oxygen species accumulation, which may contribute to increased efficacy of antitumor therapy.
Zhuonan Pu, Jinqiu Liu, Yuxuan Deng, Shuyu Hao, Xiaoli Zhang, Mingxu Yang, Chao Guo, Chao Du, Yingdan Chen, Tai Sun, Nan Ji, Zhengping Zhuang, Jie Feng   +12 more
wiley   +1 more source

CALB2 is a Mechanoresistance Gene in Metastatic Prostate Cancer

Advanced Science, EarlyView.
Prostate cancer cell lines of differing origins were cultured over many passages while being subjected to semi‐lethal levels of fluid shear stress, representing the harsh mechanical environment these cells are subjected to in the circulation during the process of distant metastasis.
Abigail R. Fabiano, Allen C. Luo, Paul Taufalele, Jenna A. Dombroski, Ehsan Aalaei, Schyler J. Rowland, Melissa S. Cantú, Alexandria T. Carter, Samantha V. Knoblauch, Cynthia A. Reinhart‐King, Michael R. King   +10 more
wiley   +1 more source

Optimized Lipid Nanoparticles with Tail‐Modified Ionizable Lipids for Safer mRNA Delivery

Advanced Science, EarlyView.
Systematic engineering of hydrophobic tail architecture in vitamin B5‐derived ionizable lipids establishes a comprehensive structure–activity relationship framework for mRNA delivery. Combined lipidtail and formulation optimization identifies TM1‐OPT3‐C, a lipid nanoparticle platform that improves efficacy–safety balance through efficient mRNA delivery,
Seo‐Hyeon Bae, Jisun Lee, Jae‐Hun Ahn, Hosam Choi, Huijeong Choi, Jungmin Kim, Minho Kang, Ho Rim Oh, So Hyun Park, Eun Young Oh, Sanghyuk Jeon, Yeeun Lee, Yu‐Sun Lee, Na‐Young Lee, Hee‐Jin Bae, Jina Kwak, Jooil Kim, Nakyung Lee, Beomsun Kang, Ji‐Hye Kim, Eunsaem Song, Daesub Song, Hyunho Yoon, Sang‐Myeong Lee, Hyewon Youn, Kiyoun Lee, Byeong‐Cheol Kang, Jae‐Hwan Nam   +27 more
wiley   +1 more source

Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging

Nature Communications, 2018
Red-shifted bioluminescence emission is needed to improve deep tissue imaging resolution. Here, the authors develop a click beetle red luciferase mutant and two naphthyl-luciferin substrates, and show the ability of the new luciferin/luciferase pairing ...
Mary P. Hall, Carolyn C. Woodroofe, Monika G. Wood, Ivo Que, Moniek van’t Root, Yanto Ridwan, Ce Shi, Thomas A. Kirkland, Lance P. Encell, Keith V. Wood, Clemens Löwik, Laura Mezzanotte   +11 more
doaj   +1 more source

Single‐cell Transcriptome Profiling Reveals Gene Regulatory Networks and Key Genes in the Root Epidermis and Cortical Cells Associated with Early Nodulation in Glycine Max

Advanced Science, EarlyView.
Single‐cell transcriptomics of soybean roots soon after rhizobial inoculation reveals epidermal and cortical cell‐specific programs and gene‐regulatory networks acting in symbiosis establishment. We identify an ethylene‐driven regulatory circuit involving WRKY6.3/6.4 transcription factors targeting select Nod19 genes that promotes infection‐thread ...
Yongbin Zhuang, Yu Geng, Xinyu Guo, Chen Wang, Yongjing Sun, Luqi Liu, Li Liu, QianQian Gao, XinFang Zhu, Hongxiang Cao, Gengshuo Zhang, Yunxia Liu, Guobin Zhang, Baoyin Chen, Jinfei Zhang, Xin Hou, Xiaoming Li, Dajian Zhang   +17 more
wiley   +1 more source

A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications

Biochemistry Research International
Bioluminescence has been a fascinating natural phenomenon of light emission from living creatures. It happens when the enzyme luciferase facilitates the oxidation of luciferin, resulting in the creation of an excited-state species that emits light ...
Asiri N. Dunuweera, Shashiprabha P. Dunuweera, K. Ranganathan   +2 more
doaj   +1 more source