Results 41 to 50 of about 67,421 (349)
Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging
Nature Methods, 2017 Red-shifted bioluminescence reporters are desirable for biological imaging. We describe the development of red-shifted luciferins based on synthetic coelenterazine analogs and corresponding mutants of NanoLuc that enable bright bioluminescence.
Hsien-Wei Yeh +5 more
semanticscholar +1 more source
Pseudo-Luciferase Activity of the SARS-CoV-2 Spike Protein for Cypridina Luciferin
ACS Central ScienceEnzymatic reactions that involve a luminescent substrate (luciferin) and enzyme (luciferase) from luminous organisms enable a luminescence detection of target proteins and cells with high specificity, albeit that conventional assay design requires a ...
Ryohei Nishihara +5 more
semanticscholar +1 more source
Luminescence of Cypridina Luciferin in the Presence of Human Plasma Alpha 1-Acid Glycoprotein
International Journal of Molecular Sciences, 2020 The enzyme Cypridina luciferase (CLase) enables Cypridina luciferin to emit light efficiently through an oxidation reaction. The catalytic mechanism on the substrate of CLase has been studied, but the details remain to be clarified. Here, we examined the
Shusei Kanie, Mami Komatsu, Y. Mitani
semanticscholar +1 more source
Automatic instrument for chemical processing to detect microorganism in biological samples by measuring light reactions [PDF]
, 1973 An automated apparatus is reported for sequentially assaying urine samples for the presence of bacterial adenosine triphosphate (ATP) that comprises a rotary table which carries a plurality of sample containing vials and automatically dispenses fluid ...
Chappelle, E. W. +3 more
core +1 more source
Orthogonal Bioluminescent Probes from Disubstituted Luciferins
Biochemistry, 2021 Bioluminescence imaging with luciferase-luciferin pairs is routinely used to monitor cellular functions. Multiple targets can be visualized in tandem using luciferases that process unique substrates, but only a handful of such orthogonal probes are known. Multiplexed studies require additional robust, light-emitting molecules.
Sierra J. Williams +2 more
openaire +5 more sources
Molecular Oncology, EarlyView.Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau +8 more
wiley +1 more source
PLoS ONE, 2023 Our recent success in the long-term maintenance of lantern shark embryos in artificial uterine systems has provided a novel option for the medical treatment of premature embryos for captive viviparous elasmobranchs.
Taketeru Tomita +5 more
doaj +1 more source
Rapid method for determination of antimicrobial susceptibilities pattern of urinary bacteria [PDF]
, 1975 Method determines bacterial sensitivity to antimicrobial agents by measuring level of adenosine triphosphate remaining in the bacteria.
Barza, M. J. +5 more
core +1 more source
Basement membrane-rich Organoids with functional human blood vessels are permissive niches for human breast cancer metastasis [PDF]
, 2013 Metastasic breast cancer is the leading cause of death by malignancy in women worldwide. Tumor metastasis is a multistep process encompassing local invasion of cancer cells at primary tumor site, intravasation into the blood vessel, survival in systemic ...
Alonso-Camino, Vanesa +12 more
core +4 more sources
Advanced Functional Materials, EarlyView.Synthesis and characterization of light‐responsive donor–acceptor Stenhouse adducts (DASA)‐coated chlorhexidine‐loaded silica nanoparticles. Such a controllable drug‐delivery system enables the release of the antimicrobial drug on demand and in consecutive cycles, thereby maintaining the concentration within the therapeutic window.
Michèle Clerc +9 more
wiley +1 more source