Results 11 to 20 of about 45,749 (264)
Expression of a multigene mushroom luciferin biosynthesis pathway as a pseudo-polycistron in plants [PDF]
Scientific ReportsMushroom bioluminescence is based on a luciferin/luciferase cycle that includes four catalytic enzymes and a post-translational modifier phosphopantetheinyl-transferase (NpgA).
David Samson +4 more
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Luciferase‐free Luciferin Electrochemiluminescence
Angewandte Chemie International Edition, 2022 AbstractLuciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation. Herein we report an electrochemical method to promote firefly's luciferin luminescence in the absence of its natural biocatalyst ...
Mattia Belotti +7 more
openaire +4 more sources
Synthesis and Evaluation of Glycosyl Luciferins [PDF]
Chemistry – A European Journal, 2023 AbstractMeasuring glycosidase activity is important to monitor any aberrations in carbohydrate hydrolase activity, but also for the screening of potential glycosidase inhibitors. To this end, synthetic substrates are needed which provide an enzyme‐dependent read‐out upon hydrolysis by the glycosidase. Herein, we present two new routes for the synthesis
Daan Sondag +8 more
openaire +2 more sources
Cells, 2023 While the suprachiasmatic nucleus (SCN) coordinates many daily rhythms, some circadian patterns of expression are controlled by SCN-independent systems. These include responses to daily methamphetamine (MAP) injections.
Riku Sato +11 more
doaj +1 more source
Structure-Functional Analysis of Human Cytochrome P450 2C8 Using Directed Evolution
Pharmaceutics, 2021 The human genome includes four cytochrome P450 2C subfamily enzymes, and CYP2C8 has generated research interest because it is subject to drug–drug interactions and various polymorphic outcomes.
Rowoon Lee +3 more
doaj +1 more source
Luciferins Under Construction: A Review of Known Biosynthetic Pathways
Frontiers in Ecology and Evolution, 2021 Bioluminescence, or the ability of a living organism to generate visible light, occurs as a result of biochemical reaction where enzyme, known as a luciferase, catalyzes the oxidation of a small-molecule substrate, known as luciferin.
Aleksandra S. Tsarkova +1 more
doaj +1 more source
Nature Communications, 2016 D-luciferin is the standard bioluminescent substrate for in vitro and in vivo imaging. Here the authors introduce AkaLumine-HCl, a soluble luciferin analogue with a near-infrared emission maximum, which allows deep tissue imaging at lower concentrations ...
Takahiro Kuchimaru +7 more
doaj +1 more source
Bioluminescence-Driven Optogenetics
Life, 2020 Bioluminescence-based technologies are among the most commonly used methods to quantify and visualise physiology at the cellular and organismal levels.
Macià Sureda-Vives, Karen S. Sarkisyan
doaj +1 more source
Electrochemically Generated Luminescence of Luminol and Luciferin in Ionic Liquids
ChemElectroChem, 2023 Electrochemiluminescence (ECL) is the generation of light triggered by an electrochemical reaction. ECL has been extensively studied in solvent‐based electrolytes, but there is a lack of data on using electrode reactions to populate an excited‐state ...
Mattia Belotti +4 more
doaj +1 more source
Synthesis of N-peptide-6-amino-D-luciferin Conjugates
Frontiers in Chemistry, 2018 A general strategy for the synthesis of N-peptide-6-amino-D-luciferin conjugates has been developed. The applicability of the strategy was demonstrated with the preparation of a known substrate, N-Z-Asp-Glu-Val-Asp-6-amino-D-luciferin (N-Z-DEVD-aLuc).
Anita K. Kovács +8 more
doaj +1 more source