Results 11 to 20 of about 44,149 (263)
Luciferase‐free Luciferin Electrochemiluminescence
Angewandte Chemie International Edition, 2022 AbstractLuciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation. Herein we report an electrochemical method to promote firefly's luciferin luminescence in the absence of its natural biocatalyst ...
Mattia Belotti +7 more
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Frontiers in Bioengineering and Biotechnology, 2021 Sensitive detection of biological events is a goal for the design and characterization of sensors that can be used in vitro and in vivo. One important second messenger is Ca++ which has been a focus of using genetically encoded Ca++ indicators (GECIs ...
Jie Yang, Carl Hirschie Johnson
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Label-free phenotypic profiling identified D-luciferin as a GPR35 agonist. [PDF]
PLoS ONE, 2012 Fluorescent and luminescent probes are essential to both in vitro molecular assays and in vivo imaging techniques, and have been extensively used to measure biological function.
Haibei Hu, Huayun Deng, Ye Fang
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Cells, 2023 While the suprachiasmatic nucleus (SCN) coordinates many daily rhythms, some circadian patterns of expression are controlled by SCN-independent systems. These include responses to daily methamphetamine (MAP) injections.
Riku Sato +11 more
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Structure-Functional Analysis of Human Cytochrome P450 2C8 Using Directed Evolution
Pharmaceutics, 2021 The human genome includes four cytochrome P450 2C subfamily enzymes, and CYP2C8 has generated research interest because it is subject to drug–drug interactions and various polymorphic outcomes.
Rowoon Lee +3 more
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Luciferins Under Construction: A Review of Known Biosynthetic Pathways
Frontiers in Ecology and Evolution, 2021 Bioluminescence, or the ability of a living organism to generate visible light, occurs as a result of biochemical reaction where enzyme, known as a luciferase, catalyzes the oxidation of a small-molecule substrate, known as luciferin.
Aleksandra S. Tsarkova +1 more
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Nature Communications, 2016 D-luciferin is the standard bioluminescent substrate for in vitro and in vivo imaging. Here the authors introduce AkaLumine-HCl, a soluble luciferin analogue with a near-infrared emission maximum, which allows deep tissue imaging at lower concentrations ...
Takahiro Kuchimaru +7 more
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Bioluminescence-Driven Optogenetics
Life, 2020 Bioluminescence-based technologies are among the most commonly used methods to quantify and visualise physiology at the cellular and organismal levels.
Macià Sureda-Vives, Karen S. Sarkisyan
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Electrochemically Generated Luminescence of Luminol and Luciferin in Ionic Liquids
ChemElectroChem, 2023 Electrochemiluminescence (ECL) is the generation of light triggered by an electrochemical reaction. ECL has been extensively studied in solvent‐based electrolytes, but there is a lack of data on using electrode reactions to populate an excited‐state ...
Mattia Belotti +4 more
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Synthesis of N-peptide-6-amino-D-luciferin Conjugates
Frontiers in Chemistry, 2018 A general strategy for the synthesis of N-peptide-6-amino-D-luciferin conjugates has been developed. The applicability of the strategy was demonstrated with the preparation of a known substrate, N-Z-Asp-Glu-Val-Asp-6-amino-D-luciferin (N-Z-DEVD-aLuc).
Anita K. Kovács +8 more
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