Exploitation of a Bacterium-Encoded Lytic Transglycosylase by a Human Oral Lytic Phage To Facilitate Infection [PDF]
Journal of Virology, 2022 The evidence for the presence of a diverse and abundant phage population in the host-associated oral microbiome came largely from metagenomic analysis or the observation of virus-like particles within saliva/plaque samples, while the isolation of oral phage and investigation of their interaction with bacterial hosts are limited.
Batbileg Bor +2 more
exaly +8 more sources
Structural Characterization of Lytic Transglycosylase SltB2 of Pseudomonas aeruginosa [PDF]
ACS OmegaLytic transglycosylases (LTs) belong to a family of enzymes that turnover the bacterial cell-wall peptidoglycan through a nonhydrolytic cleavage of the β(1-4) glycosidic bond, generating a hallmark 1,6-anhydromuramyl moiety in the reaction products.
Vega Miguel-Ruano +6 more
doaj +5 more sources
Lytic transglycosylase MltG cleaves in nascent peptidoglycan and produces short glycan strands [PDF]
Cell Surface, 2021 Bacteria encase their cytoplasmic membrane with peptidoglycan (PG) to maintain the shape of the cell and protect it from bursting. The enlargement of the PG layer is facilitated by the coordinated activities of PG synthesising and -cleaving enzymes.
Manuel Pazos +2 more
exaly +4 more sources
Inhibition of membrane-bound lytic transglycosylase B by NAG-thiazoline
FEBS Letters, 2004 The lytic transglycosylases cleave the bacterial cell wall heteropolymer peptidoglycan with the same specificity as the muramidases (lysozymes), between the N‐acetylmuramic acid and N‐acetylglucosamine residues, with the concomitant formation of a 1,6‐anhydromuramoyl residue.
Christopher W Reid, Anthony J Clarke
exaly +4 more sources
Lytic Transglycosylase Deficiency Increases Susceptibility to β-lactam Antibiotics But Reduces Susceptibility to Vancomycin in Escherichia coli. [PDF]
Microbiol ImmunolABSTRACT In Staphylococcus aureus, a gram‐positive pathogen, vancomycin‐resistant strains become susceptible to β‐lactam antibiotics, referred to as the “seesaw effect.” However, in gram‐negative bacteria, the phenomenon is less clear. Here, we analyzed the gene‐knockout effects of eight lytic transglycosylases (slt, mltA, mltB, mltC, mltD, mltE, mltF,
Kimura T +4 more
europepmc +4 more sources
X-ray Structure of Catenated Lytic Transglycosylase SltB1. [PDF]
Biochemistry, 2017 Formation of catenanes by proteins is rare, with few known examples. We report herein the X-ray structure of a catenane dimer of lytic transglycosylase SltB1 of Pseudomonas aeruginosa. The enzyme is soluble and exists in the periplasmic space, where it modifies the bacterial cell wall.
Domínguez-Gil T +5 more
europepmc +6 more sources
The lytic transglycosylase, LtgG, controls cell morphology and virulence in Burkholderia pseudomallei [PDF]
Scientific Reports, 2019 Burkholderia pseudomallei is the causative agent of the tropical disease melioidosis. Its genome encodes an arsenal of virulence factors that allow it, when required, to switch from a soil dwelling bacterium to a deadly intracellular pathogen.
Christopher H. Jenkins +8 more
doaj +6 more sources
An inhibitor/anti-inhibitor system controls the activity of lytic transglycosylase MltF in Pseudomonas aeruginosa [PDF]
mBio, 2023 Most bacterial cell envelopes contain a cell wall layer made of peptidoglycan. The synthesis of new peptidoglycan is critical for cell growth, division, and morphogenesis and is also coordinated with peptidoglycan hydrolysis to accommodate the new ...
Michelle Wang +2 more
doaj +2 more sources
Subcellular distribution of the soluble lytic transglycosylase in Escherichia coli [PDF]
Journal of Bacteriology, 1991 The localization of the major autolytic enzyme, the soluble lytic transglycosylase, in the different cell compartments of Escherichia coli was investigated by immunoelectron microscopy. Ultrathin sections were labeled with a specific antiserum against purified soluble lytic transglycosylase, and the antibody-enzyme complexes were visualized with ...
Walderich, B., Höltje, J.
openaire +4 more sources
The Lysozyme Inhibitor Thionine Acetate Is Also an Inhibitor of the Soluble Lytic Transglycosylase Slt35 from Escherichia coli [PDF]
Molecules, 2021 Lytic transglycosylases such as Slt35 from E. coli are enzymes involved in bacterial cell wall remodelling and recycling, which represent potential targets for novel antibacterial agents. Here, we investigated a series of known glycosidase inhibitors for
Aysha B. Mezoughi +8 more
doaj +2 more sources