Results 11 to 20 of about 2,643 (172)

Bulgecin A: The Key to a Broad‐Spectrum Inhibitor  That Targets Lytic Transglycosylases [PDF]

Antibiotics, 2017
Lytic transglycosylases (Lts) are involved in recycling, cell division, and metabolism of the peptidoglycan. They have been understudied for their usefulness as potential antibacterial targets due to their high redundancy in Gram‐negative bacteria ...
Allison H. Williams, Richard Wheeler, Constance Thiriau, Ahmed Haouz, Muhamed‐Kheir Taha, Ivo G. Boneca   +5 more
doaj   +3 more sources

Molecular basis of dimerization of lytic transglycosylase revealed by the crystal structure of MltA from Acinetobacter baumannii [PDF]

IUCrJ, 2021
Peptidoglycan digestion by murein-degrading enzymes is a critical process in bacterial cell growth and/or cell division. The membrane-bound lytic murein transglycosylase A (MltA) is a murein-degrading enzyme; it catalyzes the cleavage of the β-1,4 ...
Hyunseok Jang, Hackwon Do, Chang Min Kim, Gi Eob Kim, Jun Hyuck Lee, Hyun Ho Park   +5 more
doaj   +2 more sources

In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa [PDF]

Communications Biology, 2022
An extensive protein-interaction network of cell-wall biosynthesis assemblies in P. aeruginosa is elucidated using a pulldown strategy with mass-spectrometry-based proteomics and binding assays.
Luis F. Avila-Cobian, Stefania De Benedetti, Choon Kim, Rhona Feltzer, Matthew M. Champion, Jed F. Fisher, Shahriar Mobashery   +6 more
doaj   +2 more sources

EloR interacts with the lytic transglycosylase MltG at midcell in Streptococcus pneumoniae R6. [PDF]

J Bacteriol, 2021
Abstract The ellipsoid shape of Streptococcus pneumoniae is determined by the synchronized actions of the elongasome and the divisome, which have the task of creating a protective layer of peptidoglycan (PG) enveloping the cell membrane.
Winther AR, Kjos M, Herigstad ML, Håvarstein LS, Straume D.   +4 more
europepmc   +5 more sources

XAC4296 Is a Multifunctional and Exclusive Xanthomonadaceae Gene Containing a Fusion of Lytic Transglycosylase and Epimerase Domains [PDF]

Microorganisms, 2022
Microorganisms have a limited and highly adaptable repertoire of genes capable of encoding proteins containing single or variable multidomains. The phytopathogenic bacteria Xanthomonas citri subsp. citri (X.
Amanda C. P. de Oliveira, Rafael M. Ferreira, Maria Inês T. Ferro, Jesus A. Ferro, Caio Zamuner, Henrique Ferreira, Alessandro M. Varani   +6 more
doaj   +2 more sources

Crystallization of the soluble lytic transglycosylase from Escherichia coli K12 [PDF]

Journal of Molecular Biology, 1990
Lytic transglycosylases degrade the murein polymer of the bacterial cell wall to 1,6-anhydromuropeptides. These enzymes are of significant medical interest, not only because they are ideal targets for the development of new classes of antibiotics, but also because the low molecular weight products of their catalytic action can cause diverse biological ...
Rozeboom, Henriette J., Dijkstra, Bauke W., Engel, Henk, Keck, Wolfgang   +3 more
openaire   +4 more sources

A lytic transglycosylase connects bacterial focal adhesion complexes to the peptidoglycan cell wall [PDF]

eLife
The Gram-negative bacterium Myxococcus xanthus glides on solid surfaces. Dynamic bacterial focal adhesion complexes (bFACs) convert proton motive force from the inner membrane into mechanical propulsion on the cell surface.
Carlos A Ramirez Carbo, Olalekan G Faromiki, Beiyan Nan   +2 more
doaj   +2 more sources

Defective lytic transglycosylase disrupts cell morphogenesis by hindering cell wall de-O-acetylation in Neisseria meningitidis [PDF]

eLife, 2020
Lytic transglycosylases (LT) are enzymes involved in peptidoglycan (PG) remodeling. However, their contribution to cell-wall-modifying complexes and their potential as antimicrobial drug targets remains unclear.
Allison Hillary Williams, Richard Wheeler, Ala-Eddine Deghmane, Ignacio Santecchia, Ryan E Schaub, Samia Hicham, Maryse Moya Nilges, Christian Malosse, Julia Chamot-Rooke, Ahmed Haouz, Joseph P Dillard, William P Robins, Muhamed-Kheir Taha, Ivo Gomperts Boneca   +13 more
doaj   +2 more sources

Purification and properties of a membrane-bound lytic transglycosylase from Escherichia coli [PDF]

Journal of Bacteriology, 1994
A membrane-bound lytic transglycosylase (Mlt) has been solubilized in the presence of 2% Triton X-100 containing 0.5 M NaCl from membranes of an Escherichia coli mutant that carries a deletion in the slt gene coding for a 70-kDa soluble lytic transglycosylase (Slt70).
Ursinus, A. ; https://orcid.org/0009-0005-4319-2991, Höltje, J.   +1 more
core   +5 more sources

Control of the activity of the soluble lytic transglycosylase by the stringent response in Escherichia coli [PDF]

FEMS Microbiology Letters, 1990
The soluble lytic transglycosylase (Slt) of Escherichia coli is known to be a powerful murein hydrolase in vitro. It is shown here to act as an autolysin in vivo as well. Rapid autolysis of Slt overproducing cells was induced by protein biosynthesis inhibitors, which also block the fomration of guanosine-5'-diphosphate-3'-diphosphate (ppGpp).
Betzner, A., Ferreira, L., Höltje, J., Keck, W.   +3 more
openaire   +4 more sources