Results 21 to 30 of about 4,476 (193)

Structural studies and molecular dynamics simulations suggest a processive mechanism of exolytic lytic transglycosylase from Campylobacter jejuni. [PDF]

PLoS ONE, 2018
The bacterial soluble lytic transglycosylase (LT) breaks down the peptidoglycan (PG) layer during processes such as cell division. We present here crystal structures of the soluble LT Cj0843 from Campylobacter jejuni with and without bulgecin A inhibitor
Jagamya Vijayaraghavan, Vijay Kumar, Nikhil P Krishnan, Ross T Kaufhold, Ximin Zeng, Jun Lin, Focco van den Akker   +6 more
doaj   +2 more sources

Peptidoglycan recycling is critical for cell division, cell wall integrity, and β-lactam resistance in Caulobacter crescentus [PDF]

eLife
Most bacteria possess a peptidoglycan (PG) sacculus, which is continuously remodeled during cell growth and division. The turnover products generated in this process are typically imported into the cell and reused for PG biosynthesis.
Pia Richter, Anna Merz, Jacob Biboy, Nicole Paczia, Timo Glatter, Jared Ng, Waldemar Vollmer, Martin Thanbichler   +7 more
doaj   +2 more sources

Exploitation of a Bacterium-Encoded Lytic Transglycosylase by a Human Oral Lytic Phage To Facilitate Infection. [PDF]

J Virol, 2022
The evidence for the presence of a diverse and abundant phage population in the host-associated oral microbiome came largely from metagenomic analysis or the observation of virus-like particles within saliva/plaque samples, while the isolation of oral phage and investigation of their interaction with bacterial hosts are limited.
Cen L, Chang Y, Bedree JK, Ma Y, Zhong Q, Utter DR, Dong PT, Lux R, Bor B, Liu J, McLean JS, Le S, He X.   +12 more
europepmc   +5 more sources

Murein-metabolizing enzymes from Escherichia coli: existence of a second lytic transglycosylase

Journal of Bacteriology, 1992
In addition to the soluble lytic transglycosylase, a murein-metabolizing enzyme with a molecular mass of 70 kDa (Slt70), Escherichia coli possesses a second lytic transglycosylase, which has been described as a membrane-bound lytic transglycosylase (Mlt; 35 kDa; EC 3.2.1.-).
H, Engel, A J, Smink, L, van Wijngaarden, W, Keck   +3 more
openaire   +5 more sources

Lytic Transglycosylase Deficiency Increases Susceptibility to β-lactam Antibiotics But Reduces Susceptibility to Vancomycin in Escherichia coli. [PDF]

Microbiol Immunol
ABSTRACT In Staphylococcus aureus, a gram‐positive pathogen, vancomycin‐resistant strains become susceptible to β‐lactam antibiotics, referred to as the “seesaw effect.” However, in gram‐negative bacteria, the phenomenon is less clear. Here, we analyzed the gene‐knockout effects of eight lytic transglycosylases (slt, mltA, mltB, mltC, mltD, mltE, mltF,
Kimura T, Ishikawa K, Nakagawa R, Furuta K, Kaito C.   +4 more
europepmc   +2 more sources

Prevalence and mechanisms of high-level carbapenem antibiotic tolerance in clinical isolates of Klebsiella pneumoniae. [PDF]

PLoS Pathogens
Antibiotic tolerance is the ability of bacteria to survive normally lethal doses of antibiotics for extended time periods. Clinically significant Enterobacterales, for example, can exhibit high tolerance to the last-resort antibiotic meropenem. Meropenem
Trevor Cross, Facundo Torres, Nadia Nikulin, Abigail P McGee, Leena Jalees, Rhea Balakrishnan, Tolani Aliyu, Lars F Westblade, Abhyudai Singh, Tobias Dörr   +9 more
doaj   +2 more sources

Accelerated X-ray Structure Elucidation of a 36 kDa Muramidase/Transglycosylase Using wARP [PDF]

, 1998
The X-ray structure of the 36kDa soluble lytic transglycosylase from Escherichia coli has been determined starting with the multiple isomorphous replacement method with inclusion of anomalous scattering at 2.7 Å resolution. Subsequently, before any model
Asselt, Erik J. van,, Dijkstra, Bauke W.,, Kalk, Kor H.,, Lamzin, Victor S.,, Perrakis, Anastassis,   +4 more
core   +16 more sources

X-ray Structure of Catenated Lytic Transglycosylase SltB1. [PDF]

Biochemistry, 2017
Formation of catenanes by proteins is rare, with few known examples. We report herein the X-ray structure of a catenane dimer of lytic transglycosylase SltB1 of Pseudomonas aeruginosa. The enzyme is soluble and exists in the periplasmic space, where it modifies the bacterial cell wall.
Domínguez-Gil T, Molina R, Dik DA, Spink E, Mobashery S, Hermoso JA.   +5 more
europepmc   +5 more sources

The Lytic Transglycosylases of Neisseria gonorrhoeae [PDF]

Microbial Drug Resistance, 2012
Neisseria gonorrhoeae encodes five lytic transglycosylases (LTs) in the core genome, and most gonococcal strains also carry the gonococcal genetic island that encodes one or two additional LTs. These peptidoglycan (PG)-degrading enzymes are required for a number of processes that are either involved in the ...
Yolande A, Chan, Kathleen T, Hackett, Joseph P, Dillard   +2 more
openaire   +2 more sources

Growth of Porphyromonas gingivalis on human serum albumin triggers programmed cell death

Journal of Oral Microbiology, 2023
Aims Gingival crevicular fluid (GCF) constitutes the primary growth substrate for Porphyromonas gingivalis in vivo. The goal of this work was to evaluate the growth of different strains of P.
Shirin Ghods, M. Fata Moradali, Danielle Duryea, Alejandro R. Walker, Mary E. Davey   +4 more
doaj   +1 more source