Results 31 to 40 of about 4,481 (205)

Peptidoglycan lytic activity of thePseudomonas aeruginosaphage φKZ gp144 lytic transglycosylase [PDF]

FEMS Microbiology Letters, 2006
The gp144 endolysin gene from the Pseudomonas aeruginosa phage phiKZ was cloned and studies of gp144 expression into Escherichia coli showed host cell lysis. The gp144 protein was purified directly from the culture supernatant and from the bacterial cell pellet and showed in vitro antibacterial lytic activity against P. aeruginosa bacteria and degraded
Catherine Paradis‐Bleau, Isabelle Cloutier, Louis Lemieux, F. Sanschagrin, Jérà ́me Laroche, Michà ̈le Auger, Alain Garnier, Roger C. Lévesque   +7 more
openalex   +4 more sources

Coordinated Transcriptional Increases in Cell Wall Synthesis Genes in Neisseria gonorrhoeae Lacking the Lytic Transglycosylase, ltgA. [PDF]

Curr Microbiol
Broadie CO, Telchy AI, Baines AT, Dillard JP, Nicholas RA, Williams D.   +5 more
europepmc   +2 more sources

Characterization of three different lytic transglycosylases in Escherichia coli [PDF]

FEMS Microbiology Letters, 1993
Two lytic transglycosylases, releasing 1,6-anhydromuropeptides from murein sacculi are present in a mutant deleted for the soluble lytic transglycosylase 70 (Slt70). Thus, there are three different lytic transglycosylases in Escherichia coli. One of the remaining enzymes is soluble and one is a membrane protein that can be solubilized by 2% Triton X ...
Tina Romeis
openalex   +4 more sources

Characterization ofHelicobacter pyloriLytic Transglycosylases Slt and MltD [PDF]

Journal of Bacteriology, 2006
ABSTRACTPeptidoglycan (PG) is a cell wall heteropolymer that is essential for cell integrity. PG hydrolases participate in correct assembly of the PG layer and have been shown to be required for cell division, cell daughter separation, and maintenance of bacterial morphology. In silico analysis of theHelicobacter pylorigenome resulted in identification
Catherine Chaput, Agnès Labigne, Ivo G. Boneca   +2 more
openalex   +6 more sources

EloR interacts with the lytic transglycosylase MltG at midcell in Streptococcus pneumoniae R6. [PDF]

J Bacteriol, 2021
AbstractThe ellipsoid shape ofStreptococcus pneumoniaeis determined by the synchronized actions of the elongasome and the divisome, which have the task of creating a protective layer of peptidoglycan (PG) enveloping the cell membrane. The elongasome is necessary for expanding PG in the longitudinal direction whereas the divisome synthesizes the PG that
Winther AR, Kjos M, Herigstad ML, Håvarstein LS, Straume D.   +4 more
europepmc   +7 more sources

The C-terminal Domain of Escherichia coli YfhD Functions as a Lytic Transglycosylase [PDF]

Journal of Biological Chemistry, 2008
The hypothetical Escherichia coli protein YfhD has been identified as the archetype for the family 1B lytic transglycosylases despite a complete lack of experimental characterization. The yfhD gene was amplified from the genomic DNA of E. coli W3110 and cloned to encode a fusion protein with a C-terminal His(6) sequence.
Edie M. Scheurwater, Anthony J. Clarke
openalex   +5 more sources

Accelerated X-ray Structure Elucidation of a 36 kDa Muramidase/Transglycosylase Using wARP [PDF]

, 1998
The X-ray structure of the 36kDa soluble lytic transglycosylase from Escherichia coli has been determined starting with the multiple isomorphous replacement method with inclusion of anomalous scattering at 2.7 Å resolution. Subsequently, before any model
Asselt, Erik J. van,, Dijkstra, Bauke W.,, Kalk, Kor H.,, Lamzin, Victor S.,, Perrakis, Anastassis,   +4 more
core   +16 more sources

Turnover Chemistry and Structural Characterization of the Cj0843c Lytic Transglycosylase of Campylobacter jejuni. [PDF]

Biochemistry, 2021
The soluble lytic transglycosylase Cj0843c from Campylobacter jejuni breaks down cell-wall peptidoglycan (PG). Its nonhydrolytic activity sustains cell-wall remodeling and repair. We report herein our structure-function studies probing the substrate preferences and recognition by this enzyme.
Kumar V, Mathure SA, Lee M, Boorman J, Zeng X, Lin J, Hesek D, Lastochkin E, Mobashery S, van den Akker F.   +9 more
europepmc   +5 more sources

Crystallographic Studies of the Interactions of Escherichia coli Lytic Transglycosylase Slt35 with Peptidoglycan^, [PDF]

Biochemistry, 2000
Lytic transglycosylases catalyze the cleavage of the beta-1, 4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) in peptidoglycan with concomitant formation of a 1,6-anhydro bond in the MurNAc residue. To understand the reaction mechanism of Escherichia coli lytic transglycosylase Slt35, three crystal structures ...
E.J. van Asselt, Kor H. Kalk, Bauke W. Dijkstra   +2 more
openalex   +4 more sources

X-ray Structure of Catenated Lytic Transglycosylase SltB1. [PDF]

Biochemistry, 2017
Formation of catenanes by proteins is rare, with few known examples. We report herein the X-ray structure of a catenane dimer of lytic transglycosylase SltB1 of Pseudomonas aeruginosa. The enzyme is soluble and exists in the periplasmic space, where it modifies the bacterial cell wall.
Domínguez-Gil T, Molina R, Dik DA, Spink E, Mobashery S, Hermoso JA.   +5 more
europepmc   +6 more sources