Mechanism of the Escherichia coli MltE lytic transglycosylase, the cell-wall-penetrating enzyme for Type VI secretion system assembly. [PDF]
Sci Rep, 2018 Byun B +8 more
europepmc +3 more sources
The mechanistic landscape of Lytic transglycosylase as targets for antibacterial therapy
Current Opinion in Structural Biology, 2022 Lytic transglycosylases (Ltgs) are glycan strand cleaving enzymes whose role is poorly understood in the genesis of the bacterial envelope. They play multiple roles in all stages of a bacterial life cycle, by creating holes in the peptidoglycan that is necessary for cell division and separation.
Elizabeth A. Martinez-Bond +2 more
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Growth of Porphyromonas gingivalis on human serum albumin triggers programmed cell death
Journal of Oral Microbiology, 2023 Aims Gingival crevicular fluid (GCF) constitutes the primary growth substrate for Porphyromonas gingivalis in vivo. The goal of this work was to evaluate the growth of different strains of P.
Shirin Ghods +4 more
doaj +1 more source
Subcellular distribution of the soluble lytic transglycosylase in Escherichia coli [PDF]
Journal of Bacteriology, 1991 The localization of the major autolytic enzyme, the soluble lytic transglycosylase, in the different cell compartments of Escherichia coli was investigated by immunoelectron microscopy. Ultrathin sections were labeled with a specific antiserum against purified soluble lytic transglycosylase, and the antibody-enzyme complexes were visualized with ...
Brigitte Walderich +1 more
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Frontiers in Microbiology, 2023 Peptidoglycan, found within the cell wall of bacteria, is a structure critical for maintaining cell morphology and providing a protective barrier in diverse environments. Peptidoglycan is a remarkably dynamic structure that is constantly remodeled during
Beth A. Bachert, Joel A. Bozue
doaj +1 more source
The lytic transglycosylase MltB connects membrane homeostasis and in vivo fitness of Acinetobacter baumannii. [PDF]
Mol Microbiol, 2018 Crépin S +6 more
europepmc +3 more sources
Characterization of three different lytic transglycosylases inEscherichia coli [PDF]
FEMS Microbiology Letters, 1993 Two lytic transglycosylases, releasing 1,6-anhydromuropeptides from murein sacculi are present in a mutant deleted for the soluble lytic transglycosylase 70 (Slt70). Thus, there are three different lytic transglycosylases in Escherichia coli. One of the remaining enzymes is soluble and one is a membrane protein that can be solubilized by 2% Triton X ...
Romeis T, Vollmer W, Höltje J-V
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Suppression of a deletion mutation in the gene encoding essential PBP2b reveals a new lytic transglycosylase involved in peripheral peptidoglycan synthesis in Streptococcus pneumoniae D39. [PDF]
Mol Microbiol, 2016 Tsui HC +7 more
europepmc +2 more sources
Peptidoglycan lytic activity of thePseudomonas aeruginosaphage ÏKZ gp144 lytic transglycosylase [PDF]
FEMS Microbiology Letters, 2007 The gp144 endolysin gene from the Pseudomonas aeruginosa phage phiKZ was cloned and studies of gp144 expression into Escherichia coli showed host cell lysis. The gp144 protein was purified directly from the culture supernatant and from the bacterial cell pellet and showed in vitro antibacterial lytic activity against P. aeruginosa bacteria and degraded
Catherine, Paradis-Bleau +7 more
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Crystallization of the soluble lytic transglycosylase from Escherichia coli K12 [PDF]
Journal of Molecular Biology, 1990 Lytic transglycosylases degrade the murein polymer of the bacterial cell wall to 1,6-anhydromuropeptides. These enzymes are of significant medical interest, not only because they are ideal targets for the development of new classes of antibiotics, but also because the low molecular weight products of their catalytic action can cause diverse biological ...
Rozeboom, Henriette J. +3 more
openaire +3 more sources