The Mac1 ADP-ribosylhydrolase is a therapeutic target for SARS-CoV-2 [PDF]
eLifeSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to pose a threat to public health. Current therapeutics remain limited to direct-acting antivirals that lack distinct mechanisms of action and are already showing signs of viral ...
Rahul K Suryawanshi +30 more
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Identification of a series of pyrrolo-pyrimidine-based SARS-CoV-2 Mac1 inhibitors that repress coronavirus replication [PDF]
mBioCoronaviruses (CoVs) can emerge from zoonotic sources and cause severe diseases in humans and animals. CoVs encode for a macrodomain (Mac1) that binds to and removes ADP-ribose from target proteins.
Jessica J. Pfannenstiel +18 more
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Examining the Effects of the RUNX1 p.Leu43Ser Variant on FPD/AML Phenotypes Using a CRISPR/Cas9-Generated Knock-In Murine Model [PDF]
BiomoleculesGermline heterozygous variants in RUNX1 lead to Familial Platelet Disorder with Myeloid Leukemia Predisposition (FPD/AML). Cellular and/or animal models are helpful to uncovering the role of a variant in disease progression. Twenty-five mice per genotype
Ana Marin-Quilez +11 more
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Molecular basis for loss of virulence in Magnaporthe oryzae strain AM16 [PDF]
Frontiers in Plant ScienceThe rapid virulence variation of Magnaporthe oryzae (M. oryzae) to rice is a big challenge for rice blast control. Even though many studies have been done by scientists all over the world, the mechanism of virulence variation in M. oryzae remains elusive.
Jiahui Deng +6 more
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Copper acquisition is essential for plant colonization and virulence in a root-infecting vascular wilt fungus. [PDF]
PLoS PathogensPlant pathogenic fungi provoke devastating agricultural losses and are difficult to control. How these organisms acquire micronutrients during growth in the host environment remains poorly understood.
Rafael Palos-Fernández +7 more
doaj +2 more sources
Neuroprotective Potential of New Monoterpene-Adamatane Conjugates—A Pilot Study [PDF]
Current Issues in Molecular BiologyNeurodegenerative diseases, including Alzheimer’s disease, are marked by cholinergic dysfunction, oxidative stress, and reduced neurotrophic support, which drives the quest for multifunctional therapeutic agents.
Stela Dragomanova +18 more
doaj +2 more sources
Effects of extracellular metabolic acidosis and out-of-equilibrium CO2/HCO3− solutions on intracellular pH in cultured rat hippocampal neurons [PDF]
Frontiers in PhysiologyMetabolic acidosis (MAc)—an extracellular pH (pHo) decrease caused by a [HCO3−]o decrease at constant [CO2]o—usually causes intracellular pH (pHi) to fall.
Patrice G. Bouyer +8 more
doaj +2 more sources
Recombinant infectious bronchitis virus containing mutations in non-structural proteins 10, 14, 15, and 16 and within the macrodomain provides complete protection against homologous challenge [PDF]
Journal of VirologyInfectious bronchitis virus (IBV) is the etiological agent of infectious bronchitis, an acute highly contagious economically important disease of chickens.
Sarah Keep +12 more
doaj +2 more sources
Journal of Medicinal Chemistry, 2023 Abstract The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has made it clear that further development of antiviral therapies will be needed to combat additional SARS-CoV-2 variants or novel CoVs.
Sarah Wazir +11 more
openaire +3 more sources
High-Throughput Activity Assay for Screening Inhibitors of the SARS-CoV-2 Mac1 Macrodomain [PDF]
ACS Chemical Biology, 2021 Macrodomains are a class of conserved ADP-ribosylhydrolases expressed by viruses of pandemic concern, including coronaviruses and alphaviruses. Viral macrodomains are critical for replication and virus-induced pathogenesis; therefore, these enzymes are a promising target for antiviral therapy.
Morgan Dasovich +16 more
openaire +4 more sources