Results 71 to 80 of about 1,703 (147)

Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes

Advances in Bioinformatics, Volume 2014, Issue 1, 2014., 2014
Background. Targeted enrichment improves coverage of highly mutable viruses at low concentration in complex samples. Degenerate primers that anneal to conserved regions can facilitate amplification of divergent, low concentration variants, even when the strain present is unknown. Results.
Shea N. Gardner, Crystal J. Jaing, Maher M. Elsheikh, José Peña, David A. Hysom, Monica K. Borucki, Paul Harrison   +6 more
wiley   +1 more source

Elucidating the pivotal role of convalescent plasma therapy in critically ill COVID-19 patients: A review

Hematology Reports, 2020
World Health Organization (WHO) declared coronavirus disease (COVID-19) a pandemic in March 2020. Currently almost every country in the world has reported cases with moderate to high mortality rates. The European Union (EU), the United States of America (
Seidu A. Richard, Sylvanus Kampo, Maite Esquijarosa Hechavarria   +2 more
doaj   +1 more source

Family‐Specific Degenerate Primer Design: A Tool to Design Consensus Degenerated Oligonucleotides

Biotechnology Research International, Volume 2013, Issue 1, 2013., 2013
Designing degenerate PCR primers for templates of unknown nucleotide sequence may be a very difficult task. In this paper, we present a new method to design degenerate primers, implemented in family‐specific degenerate primer design (FAS‐DPD) computer software, for which the starting point is a multiple alignment of related amino acids or nucleotide ...
Javier Alonso Iserte, Betina Ines Stephan, Sandra Elizabeth Goñi, Cristina Silvia Borio, Pablo Daniel Ghiringhelli, Mario Enrique Lozano, Goetz Laible   +6 more
wiley   +1 more source

Arenavirus Budding

Advances in Virology, Volume 2011, Issue 1, 2011., 2011
Several arenaviruses cause hemorrhagic fever disease in humans and pose a significant public health concern in their endemic regions. On the other hand, the prototypic arenavirus LCMV is a superb workhorse for the investigation of virus‐host interactions and associated disease.
Shuzo Urata, Juan Carlos de la Torre, Fadila Bouamr   +2 more
wiley   +1 more source

Urgent pandemic messaging of WHO, World Bank, and G20 is inconsistent with their evidence base

Global Policy, Volume 15, Issue 4, Page 689-707, September 2024.
Abstract When international agencies make claims of an “existential threat” to humanity and advocate for urgent action from countries, it should be a safe assumption that they are consistent with their own data. However, a review of the data and evidentiary citations underlying the claims of the World Health Organization (WHO), the World Bank, and the ...
David Bell, Garrett Wallace Brown, Jean von Agris, Blagovesta Tacheva   +3 more
wiley   +1 more source

Expression and Purification of Z Protein from Junín Virus

BioMed Research International, Volume 2010, Issue 1, 2010., 2010
Arenaviridae comprises 23 recognized virus species with a bipartite ssRNA genome and an ambisense coding strategy. The virions are enveloped and include nonequimolar amounts of each genomic RNA species, designated L and S, coding for four ORFs (N, GPC, L, and Z).
S. E. Goñi, C. S. Borio, F. B. Romano, R. P. Rota, M. G. Pilloff, J. A. Iserte, M. A. Tortorici, B. I. Stephan, M. F. Bilen, P. D. Ghiringhelli, M. E. Lozano, Norbert K. Herzog   +11 more
wiley   +1 more source

Virions at the gates: receptors and the host-virus arms race. [PDF]

PLoS Biology, 2013
All viruses need to bind to specific receptor molecules on the surface of target cells to initiate infection. Virus-receptor binding is highly specific, and this specificity determines both the species and the cell type that can be infected by a given ...
John M Coffin
doaj   +1 more source

Animal Models of Pathogenic New World Arenaviruses

Microorganisms
Since the emergence of Junín virus in 1953, pathogenic New World arenaviruses have remained a public health concern. These viruses, which also include Machupo virus, Guanarito virus, Sabiá virus, and Chapare virus, cause acute viral hemorrhagic fever and
Alexander V. Alvarado, Robert W. Cross, Thomas W. Geisbert, Courtney Woolsey   +3 more
doaj   +1 more source

Glycoprotein N-linked glycans play a critical role in arenavirus pathogenicity.

PLoS Pathogens, 2021
Several arenaviruses cause hemorrhagic fevers in humans with high case fatality rates. A vaccine named Candid#1 is available only against Junin virus (JUNV) in Argentina.
Takaaki Koma, Cheng Huang, Adrian Coscia, Steven Hallam, John T Manning, Junki Maruyama, Aida G Walker, Milagros Miller, Jeanon N Smith, Michael Patterson, Jonathan Abraham, Slobodan Paessler   +11 more
doaj   +1 more source

The Current State of Vaccine Development for Specific Prophylactics of Arenaviral Hemorrhagic Fevers

Проблемы особо опасных инфекций, 2018
Presently, the Arenavirus genus (Arenaviridae family) includes 26 individual species of viruses. It is divided into two main groups – Old World arenaviruses and New World arenaviruses.
T. E. Sizikova, V. N. Lebedev, S. I. Syromyatnikova, S. V. Borisevich   +3 more
doaj   +1 more source