Results 11 to 20 of about 187,471 (221)

One section, two worlds: single-cell integration of MALDI-MSI and spatial transcriptomics on the same single tissue section [PDF]

Scientific Reports
Understanding tissue complexity requires spatially resolved multi-omics data at single-cell resolution. Here, we present a workflow integrating high-resolution matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) with Xenium ...
Tim F. E. Hendriks, Gert B. Eijkel, Theodoros Visvikis, Benjamin Balluff, Ron M. A. Heeren, Eva Cuypers   +5 more
doaj   +3 more sources

Advancing atherosclerosis research: The Power of lipid imaging with MALDI-MSI

Atherosclerosis
Atherosclerosis is a chronic inflammatory disease that is one of the leading causes of mortality globally. It is characterized by the formation of atheromatous plaques in the intima layer of larger arteries. The (fibro-)fatty plaques usually develop asymptomatically within the vessel until a serious event such as myocardial infarction or stroke occurs.
Maria Vinaixa, Luis Masana, Christoph Bookmeyer   +2 more
exaly   +5 more sources

Enhancing Metabolite Coverage in MALDI-MSI using Laser Post-Ionisation (MALDI-2). [PDF]

Analytical Methods, 2023
Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) of metabolites can reveal how metabolism is altered throughout heterogeneous tissues.
Theodore, Alexandrov, Heloisa, Milioli, Carley, Purcell, Shane, Ellis, Bishoy, Wadie, Christine, Chaffer, Todd, Mitchell, Jayden, Mckinnon   +7 more
core   +3 more sources

Molecular Profiling of Glioblastoma Patient-Derived Single Cells Using Combined MALDI-MSI and MALDI-IHC. [PDF]

Anal Chem
In recent years, mass spectrometry-based imaging techniques have improved at unprecedented speeds, particularly in spatial resolution, and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) experiments can now routinely ...
Krestensen KK, Hendriks TFE, Grgic A, Derweduwe M, De Smet F, Heeren RMA, Cuypers E.   +6 more
europepmc   +3 more sources

MALDI-MSI-LC-MS/MS Workflow for Single-Section Single Step Combined Proteomics and Quantitative Lipidomics. [PDF]

Anal Chem
We introduce a novel approach for comprehensive molecular profiling in biological samples. Our single-section methodology combines quantitative mass spectrometry imaging (Q-MSI) and a single step extraction protocol enabling lipidomic and proteomic ...
Hendriks TFE, Krestensen KK, Mohren R, Vandenbosch M, De Vleeschouwer S, Heeren RMA, Cuypers E.   +6 more
europepmc   +3 more sources

MALDI MSI Protocol for Spatial Bottom-Up Proteomics at Single-Cell Resolution [PDF]

Journal of Proteome Research
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) started with spatial mapping of peptides and proteins. Since then, numerous bottom-up protocols have been developed.
Shane R Ellis, Eva Cuypers, Ron Heeren
exaly   +3 more sources

Enhanced Spatial Mapping of Histone Proteoforms in Human Kidney Through MALDI-MSI by High-Field UHMR Orbitrap Detection [PDF]

Analytical Chemistry, 2022
Core histones including H2A, H2B, H3, and H4 are key modulators of cellular repair, transcription, and replication within eukaryotic cells, playing vital roles within the pathogenesis of disease and cellular responses to environmental stimuli ...
Alexander, Makarov, Dušan, Veličković, Yanli, Ding, Kumar, Sharma, Mowei, Zhou, Dharam, Kaushik, William, Kew, Kyle, Fort, Kevin, Zemaitis, Annapurna, Pamreddy, Ljiljana, Paša-Tolić, Maria, Reinhardt-Szyba   +11 more
core   +3 more sources

Chemical Mapping of the Degradation of Geranium Lake in Paint Cross Sections by MALDI-MSI

Analytical Chemistry, 2023
Matrix assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has become a powerful method to extract spatially resolved chemical information in complex materials.
Ermanno Avranovich Clerici (17444416), Alba Alvarez-Martin (7162883), Jusal Quanico (1917355), Teresa Scovacricchi (17444413), Koen Janssens (219438), Geert Baggerman (702592)   +5 more
core   +2 more sources

Improved Mass Calibration in MALDI MSI Using Neural Network-Based Recalibration. [PDF]

Anal Chem
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is a powerful imaging method for generating molecular maps of biological samples and has numerous applications in biomedical research.
Denker A, Behrmann J, Boskamp T.
europepmc   +5 more sources

Simultaneous metabolite MALDI-MSI, whole exome and transcriptome analysis from formalin-fixed paraffin-embedded tissue sections.

Laboratory Investigation, 2022
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) allows spatial analysis of proteins, metabolites, or small molecules from tissue sections.
Belka, C., Baumeister, Philipp, Heider, Theresa, Unger, K., Riedl, T., Riedl, Tobias, Kreutzer, Lisa, Baumeister, P., Walch, Axel, Zitzelsberger, Horst, Klauschen, F., Heikenwälder, Mathias, Unger, Kristian, Hess, Julia, Klauschen, Frederick, Heider, T., Walch, A.K., Zitzelsberger, H., Weber, Peter, Kreutzer, L., Hess-Rieger, J., Weber, P., Belka, Claus, Heikenwaelder, M.   +23 more
core   +2 more sources