Results 111 to 120 of about 11,749,169 (183)

ATPase activity and DNA binding by Mcm2-7_3K499A.

, 2013
(A) The reconstituted Mcm2-7 and Mcm2-73K499A complexes were tested for ATPase activity by incubating with [γ32P]-ATP and measuring the radioactivity of free Pi produced at the time points indicated.
Tina L. Sing (496154), Christopher J. Brandl (496156), Brian H. Shilton (496155), Megan J. Davey (496157), Simon K. W. Lam (496153), Xiaoli Ma (88826)   +5 more
core   +1 more source

A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2-7 onto DNA [PDF]

, 2014
01.09.14 KB. Embargo period of 6 months before published version can be deposited.The regulated loading of the replicative helicase minichromosome maintenance proteins 2–7 (MCM2–7) onto replication origins is a prerequisite for replication fork ...
Sun, Jingchuan, Herrera, M Carmen, Tognetti, Silvia, Samel, Stefan A, Speck, Christian, Riera, Alberto, Fernandez-Cid, Alejandra, Li, Huilin   +7 more
core   +1 more source

Rethinking origin licensing

eLife, 2017
Human cells that lack a subunit in their origin recognition complex are viable, which suggests the existence of alternative mechanisms to initiate DNA replication.
Stephen P Bell
doaj   +1 more source

Regulation of eukaryotic Mcm2-7 activity [PDF]

, 2013
The transfer of genetic material from one cell generation to the next requires precise genome duplication. Aberrant DNA replication can lead to genomic instability and contribute to diseases arising from an unregulated cell cycle, such as cancer.
openaire  

Chromosomal Mcm2-7 distribution is the primary driver of the genome replication program in species from yeast to humans

, 2019
The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading of pre-replication complexes (pre-RCs) across the genome at the onset of S-phase, and by differences in the timing of activation of ...
E. Foss, S. Sripathy, Tonibelle Gatbonton-Schwager, Hyunchang Kwak, A. Thiesen, Uyen Lao, A. Bedalov, A. Bedalov   +7 more
semanticscholar   +1 more source

MCM2-7 Form Double Hexamers at Licensed Origins in Xenopus Egg Extract

, 2011
In late mitosis and G1, Mcm2-7 are assembled onto replication origins to license them for initiation in the upcoming S phase. After initiation, Mcm2-7 provide helicase activity to unwind DNA at the replication fork.
Blow, J. Julian; id_orcid, Richard C. Jones, Khoudoli, Guennadi A., Guennadi A. Khoudoli, Jones, Richard C, Blow, J Julian, Jones, Richard C., J. Julian Blow, Gambus, Agnieszka; id_orcid, Blow, J. Julian, Gambus, Agnieszka, Khoudoli, Guennadi A, Agnieszka Gambus   +12 more
core   +1 more source

CRL4DCAF12 regulation of MCMBP ensures optimal licensing of DNA replication

Nature Communications
The minichromosome maintenance (MCM2-7) protein complexes are central drivers of genome duplication. Distinct protein pools, parental and nascent MCMs, and their precise equilibrium are essential to sustain error-free DNA replication.
Anoop Kumar Yadav, Alikhan Abdirov, Katarina Ondruskova, Simran Negi, Kristina Jamrichova, Karolina Kolarova, Nikol Dibus, Jana Krejci, Hana Polasek-Sedlackova, Lukas Cermak   +9 more
doaj   +1 more source

Bioinformatic Analysis of the Expression and Clinical Significance of the DNA Replication Regulator MCM Complex in Bladder Cancer

International Journal of General Medicine, 2022
Ru Chen,1,2,* Bing Hu,1,* Ming Jiang,1,* Wen Deng,1 Ping Zheng,3 Bin Fu1,4 1Department of Urology, the First Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province, People’s Republic of China; 2Department of Urology, The ...
Chen R, Hu B, Jiang M, Deng W, Zheng P, Fu B   +5 more
doaj  

Geminin inhibits DNA replication licensing by sterically blocking CDT1-MCM2 interactions

Nature Communications
DNA replication is tightly regulated to occur once per cell cycle, with the MCM2-7 helicase loaded onto replication origins only during G1-phase. In higher eukaryotes, geminin negatively regulates this process during S-, G2- and M-phases by binding the ...
Joshua Tomkins, Lucy V. Edwardes, Sarah V. Faull, Matthew Peach, Peter J. Gillespie, Vera Leber, Anna Schmidt, Halil Bounoua, Nicholas Sim, Rosa Camarillo, J. Julian Blow, Alexis R. Barr, Anna Barnard, Christian Speck   +13 more
doaj   +1 more source

Dormant origins licensed by excess Mcm2-7 are required for human cells to survive replicative stress

, 2007
In late mitosis and early G1, Mcm2-7 complexes are loaded onto DNA to license replication origins for use in the upcoming S phase. However, the amount of Mcm2-7 loaded is in significant excess over the number of origins normally used.
Blow, J. Julian; id_orcid, Jackson, Dean A., Ge, Xin Quan, Blow, J. Julian   +3 more
core   +1 more source