Results 1 to 10 of about 57,465 (182)

Sialylated and sulfated N-Glycans in MDCK and engineered MDCK cells for influenza virus studies [PDF]

Scientific Reports, 2022
The Madin-Darby canine kidney (MDCK) cell line is an in vitro model for influenza A virus (IAV) infection and propagation. MDCK-SIAT1 (SIAT1) and humanized MDCK (hCK) cell lines are engineered MDCK cells that express N-glycans with elevated levels of ...
Lauren Byrd-Leotis, Nan Jia, Yasuyuki Matsumoto, Dongli Lu, Yoshihiro Kawaoka, David A. Steinhauer, Richard D. Cummings   +6 more
doaj   +4 more sources

Metabolomics profiling reveals differences in proliferation between tumorigenic and non-tumorigenic Madin-Darby canine kidney (MDCK) cells [PDF]

PeerJ, 2023
Background Madin-Darby canine kidney (MDCK) cells are a cellular matrix in the production of influenza vaccines. The proliferation rate of MDCK cells is one of the critical factors that determine the vaccine production cycle.
Na Sun, Yuchuan Zhang, Jian Dong, Geng Liu, Zhenbin Liu, Jiamin Wang, Zilin Qiao, Jiayou Zhang, Kai Duan, Xuanxuan Nian, Zhongren Ma, Xiaoming Yang   +11 more
doaj   +3 more sources

TGM2 inhibits the proliferation, migration and tumorigenesis of MDCK cells. [PDF]

PLoS ONE, 2023
Madin-Darby canine kidney (MDCK) cells are one of the main cell lines used for influenza vaccine production due to their high virus yield and low mutation resistance.
Zhenyu Qiu, Shouqing Guo, Geng Liu, Mengyuan Pei, Yuejiao Liao, Jiamin Wang, Jiayou Zhang, Di Yang, Zilin Qiao, Zhuo Li, Zhongren Ma, Zhenbin Liu, Xiaoming Yang   +12 more
doaj   +2 more sources

TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus [PDF]

Heliyon, 2023
Tumor necrosis factor receptor-associated factor 3 (TRAF3), an adaptor protein, has significant and varying effects on immunity depending on cell types. The role of TRAF3 in Madin-Darby Canine Kidney Epithelial (MDCK) cell resistance to influenza A virus
Yang Le, Jiayou Zhang, Zheng Gong, Zhegang Zhang, Xuanxuan Nian, Xuedan Li, Daiguan Yu, Ning Ma, Rong Zhou, Guomei Zhang, Bo Liu, Lu Yang, Baiqi Fu, Xiuqin Xu, Xiaoming Yang   +14 more
doaj   +2 more sources

Tumorigenicity decrease in Bcl-xL deficient MDCK cells ensuring the safety for influenza vaccine production. [PDF]

PLoS ONE
Madin-Darby canine kidney (MDCK) cells are the recognized cell strain for influenza vaccine production. However, the tumorigenic potential of MDCK cells raises concerns about their use in biological product manufacturing.
Jiahao Zheng, Boran Li, Lanxin Jia, Jiayou Zhang, Zheng Gong, Yang Le, Xuanxuan Nian, Xuedan Li, Bo Liu, Daiguan Yu, Zhegang Zhang, Changgui Li   +11 more
doaj   +2 more sources

Reduction in Interferon-Stimulated Genes Contributes to High-Yield Production of Influenza Virus in Suspension MDCK Cells [PDF]

Vaccines
Compared with the traditional vaccine produced in embryonated chicken eggs, cell-based manufacturing represented by the Madin–Darby canine kidney (MDCK) cell line has a larger production scale and reduces the risk of egg shortage in a pandemic ...
Qi Wang, Jian Luo, Beibei Li, Qian Ye, Wenting Xu, Feixia Gao, Linting Zhou, Wenyue Lu, Wen-Song Tan, Xiuling Li   +9 more
doaj   +2 more sources

Development of high-growth influenza H7N9 prepandemic candidate vaccine viruses in suspension MDCK cells [PDF]

Journal of Biomedical Science, 2020
Background Influenza vaccine manufacturers traditionally use egg-derived candidate vaccine viruses (CVVs) to produce high-yield influenza viruses for seasonal or pandemic vaccines; however, these egg-derived CVVs need an adaptation process for the virus ...
Tsai-Teng Tzeng, Po-Ling Chen, Tsai-Chuan Weng, Shin-Yi Tsai, Chia-Chun Lai, Hsin-I Chou, Pin-Wen Chen, Chia-Chun Lu, Ming-Tsan Liu, Wang-Chou Sung, Min-Shi Lee, Alan Yung-Chih Hu   +11 more
doaj   +2 more sources

IIIG9 and PP1α form a protein complex in the adherens junctions of polarized ependymal and MDCK cells [PDF]

Fluids and Barriers of the CNS
Background IIIG9 was originally identified as a novel regulatory subunit of protein phosphatase 1 (PP1, PPP1R32) in ependymal cells, where it localizes to cilia and adherens junctions.
María José Oviedo, Eder Ramírez, Isabelle De Lima, Jesús Mateo Grondona, Manuel Cifuentes, Fernando Martínez, María José Barahona, Francisco Nualart, Katterine Salazar   +8 more
doaj   +2 more sources

Claudin-4 knockout by TALEN-mediated gene targeting in MDCK cells: Claudin-4 is dispensable for the permeability properties of tight junctions in wild-type MDCK cells. [PDF]

PLoS ONE, 2017
Epithelia act as a barrier between the internal and external environments, and the movement of substances via the paracellular pathway is regulated by tight junctions (TJs). Claudins are major determinants of TJ permeability.
Shinsaku Tokuda, Toyohiro Hirai, Mikio Furuse   +2 more
doaj   +2 more sources

IRF7-deficient MDCK cell based on CRISPR/Cas9 technology for enhancing influenza virus replication and improving vaccine production [PDF]

PeerJ, 2022
The influenza virus is a cause of seasonal epidemic disease and enormous economic injury. The best way to control influenza outbreaks is through vaccination.
Oraphan Mayuramart, Witthaya Poomipak, Somruthai Rattanaburi, Kritsada Khongnomnan, Songtham Anuntakarun, Suthat Saengchoowong, Tanit Chavalit, Naphat Chantaravisoot, Sunchai Payungporn   +8 more
doaj   +2 more sources