Identification and comparison of m6A modifications in glioblastoma non-coding RNAs with MeRIP-seq and Nanopore dRNA-seq [PDF]
Epigenetics, 2023 The most prominent RNA modification – N6-methyladenosine (m6A) – affects gene regulation and cancer progression. The extent and effect of m6A on long non-coding RNAs (lncRNAs) is, however, still not clear. The most established method for m6A detection is
Rytis Stakaitis +2 more
exaly +6 more sources
RADAR: differential analysis of MeRIP-seq data with a random effect model [PDF]
Genome Biology, 2019 Epitranscriptome profiling using MeRIP-seq is a powerful technique for in vivo functional studies of reversible RNA modifications. We develop RADAR, a comprehensive analytical tool for detecting differentially methylated loci in MeRIP-seq data.
Rohit N Kulkarni +2 more
exaly +8 more sources
MoAIMS: efficient software for detection of enriched regions of MeRIP-Seq [PDF]
BMC Bioinformatics, 2020 Background Methylated RNA immunoprecipitation sequencing (MeRIP-Seq) is a popular sequencing method for studying RNA modifications and, in particular, for N6-methyladenosine (m6A), the most abundant RNA methylation modification found in various species ...
Yiqian Zhang, Michiaki Hamada
doaj +5 more sources
Validated Impacts of N6-Methyladenosine Methylated mRNAs on Apoptosis and Angiogenesis in Myocardial Infarction Based on MeRIP-Seq Analysis [PDF]
Frontiers in Molecular Biosciences, 2022 Objectives: N6-methyladenosine (m6A) is hypothesized to play a role in the regulation of pathogenesis of myocardial infarction (MI). This study was designed to compare m6A-tagged transcript profiles to identify mRNA-specific changes on pathophysiological
Yingjie Zhang +11 more
doaj +5 more sources
Comprehensive Analysis of N6-Methyladenosine Methylation in Transverse Aortic Constriction-Induced Cardiac Fibrosis Based on MeRIP-Seq Analysis [PDF]
BiomedicinesBackground: The function and mechanism of N6-methyladenosine (m6A) methylation in pressure-overload cardiac fibrosis remains limited and unclear. This study aims to analyze and predict m6A modifications present in mouse hearts because of transverse aortic constriction (TAC).
Bing Song, Pengying Zhao, Cuntao Yu
exaly +6 more sources
m6A methylation profiling as a prognostic marker in nasopharyngeal carcinoma: insights from MeRIP-Seq and RNA-Seq [PDF]
Frontiers in ImmunologyBackgroundNasopharyngeal carcinoma (NPC) is a type of malignant tumors commonly found in Southeast Asia and China, with insidious onset and clinical symptoms.
Xiaochuan Chen +8 more
doaj +4 more sources
Differential RNA methylation analysis for MeRIP-seq data under general experimental design. [PDF]
Bioinformatics, 2022 AbstractMotivationRNA epigenetics is an emerging field to study the post-transcriptional gene regulation. The dynamics of RNA epigenetic modification have been reported to associate with many human diseases. Recently developed high-throughput technology named Methylated RNA Immunoprecipitation Sequencing (MeRIP-seq) enables the transcriptome-wide ...
Peng Jin, Hao Wu
exaly +4 more sources
Statistical modeling of immunoprecipitation efficiency of MeRIP-seq data enabled accurate detection and quantification of epitranscriptome [PDF]
Computational and Structural Biotechnology JournalAbstract Background Recent advancements in epitranscriptomics highlight reversible RNA modifications as crucial regulators, with N6-methyladenosine (m 6 A) being abundant in eukaryotic mRNAs.
Jionglong Su, Yue Wang, Anh Nguyen
exaly +5 more sources
Transcriptome-Wide m6A Methylation Landscape of Longissimus Dorsi Muscle in Indigenous Guizhou Cattle [PDF]
LifeGuizhou cattle are important indigenous bovine genetic resources for regional beef production and germplasm conservation. This study characterized the transcriptome-wide N6-methyladenosine (m6A) enrichment landscape in longissimus dorsi muscle from three
Junda Wu +7 more
doaj +2 more sources
Integrated analysis of autophagy and ferroptosis mediating aristolochic acid A-induced nephrotoxicity via transcriptome and m6A epigenetic transcriptome in HK-2 cells [PDF]
Frontiers in GeneticsIntroductionAristolochic acid A possesses anti-inflammatory, antispasmodic and diuretic pharmacological activities, while exerting strong toxic effects that cause irreversible renal tubular injury, renal fibrosis and malignant tumors such as urothelial ...
Peichun Ye +10 more
doaj +2 more sources