Results 41 to 50 of about 152,412 (174)

Detecting differential usage of exons from RNA-Seq data [PDF]

, 2012
RNA-Seq is a powerful tool for the study of alternative splicing and other forms of alternative isoform expression. Understanding the regulation of these processes requires comparisons between treatments, tissues or conditions.
Simon Anders, Wolfgang Huber, Alejandro Reyes   +2 more
core   +1 more source

FTO suppresses glycolysis and growth of papillary thyroid cancer via decreasing stability of APOE mRNA in an N6-methyladenosine-dependent manner

Journal of Experimental & Clinical Cancer Research, 2022
Background N6-methyladenosine (m6A) modification is the most common chemical modification in mammalian mRNAs, and it plays important roles by regulating several cellular processes.
Jiapeng Huang, Wei Sun, Zhihong Wang, Chengzhou Lv, Ting Zhang, Dalin Zhang, Wenwu Dong, Liang Shao, Liang He, Xiaoyu Ji, Ping Zhang, Hao Zhang   +11 more
doaj   +1 more source

m6A Methylation Analysis of Differentially Expressed Genes in Skin Tissues of Coarse and Fine Type Liaoning Cashmere Goats

Frontiers in Genetics, 2020
N6-methyladenosine (m6A) is the most common internal modification in mRNAs of all higher eukaryotes. Here we perform two high-throughput sequencing methods, m6A-modified RNA immunoprecipitation sequence (MeRIP-seq) and RNA sequence (RNA-seq) to identify ...
Yanru Wang, Yuanyuan Zheng, Dan Guo, Xinghui Zhang, Suling Guo, Taiyu Hui, Chang Yue, Jiaming Sun, Suping Guo, Zhixian Bai, Weidong Cai, Xinjiang Zhang, Yixing Fan, Zeying Wang, Wenlin Bai   +14 more
doaj   +1 more source

Methods, Challenges and Potentials of Single Cell RNA-seq [PDF]

, 2012
RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene
Daniel Hebenstreit, Hebenstreit, Daniel
core   +1 more source

Hepatic Lipid Stress Drives Metabolic Dysfunction‐Associated Steatohepatitis Progression through FTO O‐GlcNAcylation‐Induced m6A Demethylation of HRAS [PDF]

Advanced science
Lipid stress drives O‐GlcNAcylation of the m6A demethylase FTO at Ser195, enhancing its stability and demethylase activity. This modification removes m6A from HRAS mRNA, preventing its degradation and activating PI3K/AKT signaling to promote hepatic steatosis and inflammation.
Yin J, Zhang Y, Liu Q, Liu R, Yuan H, Chen S, Zheng M, Wang K, Tang N, Huang A.   +9 more
europepmc   +2 more sources

Accounting for immunoprecipitation efficiencies in the statistical analysis of ChIP-seq data [PDF]

, 2013
Background: ImmunoPrecipitation (IP) efficiencies may vary largely between different antibodies and between repeated experiments with the same antibody.
Bao, Yanchun,, ’t Hoen, Peter AC, Bao, Yanchun, Veronica Vinciotti, Hoen, P.A.C. 't, Vinciotti, V., Vinciotti, Veronica,, Yanchun Bao, Wit, Ernst,, Bao, Y.C., Ernst Wit, Wit, Ernst, Vinciotti, V, Peter AC ’t Hoen, 't Hoen, Peter A C, Vinciotti, Veronica, Hoen, Peter A.C. ’t,, Bao, Y, 't Hoen, PAC, Wit, E, Wit, E.   +20 more
core   +2 more sources

DataSheet1_Validated Impacts of N6-Methyladenosine Methylated mRNAs on Apoptosis and Angiogenesis in Myocardial Infarction Based on MeRIP-Seq Analysis.docx

, 2022
Objectives: N6-methyladenosine (m6A) is hypothesized to play a role in the regulation of pathogenesis of myocardial infarction (MI). This study was designed to compare m6A-tagged transcript profiles to identify mRNA-specific changes on pathophysiological
Meiling Teng (11529268), Xiao Lu (355468), Yu Zheng (2950), Yini Dang (654078), Jiayue Wang (3136224), Yihui Cheng (5189663), Yingjie Zhang (381556), Zihao Kong (12011810), Xiu Zhang (1804195), Shenrui Wang (11529271), Wenjie Hua (7848546), Min Zhang (111999)   +11 more
core   +1 more source

MeRIP-seq and RNA-seq data of mycelium and spore of Ascosphaera apis , a widespread bee fungal pathogen [PDF]

, 2020
ABSTRACT Ascosphaera apis is widespread fungal pathogen of honeybee larvae, causing chalkbrood, a chronic disease that weakens bee health and colony productivity. In this article, mecylia and spores of A.
Du, Yu, Jiang, Haibin, Zhu, Zhiwei, Wang, Jie, Chen, Huazhi, Xiong, Cuiling, Zheng, Yanzhen, Chen, Dafu, Guo, Rui   +8 more
openaire   +1 more source

YBX1 Promotes Malignant Progression of HNSCC via Stabilizing NSUN2-m5C Modified ATF4 mRNA. [PDF]

Cancer Sci
The diagram depicts the core axis and the key finding: “Targeting YBX1/NSUN2 abrogates HNSCC progression”. Within left‐sided HNSCC cells, NSUN2 mediates m5C methylation on the CDS of ATF4 mRNA; YBX1 then specifically recognizes and binds this modified site, augmenting ATF4 mRNA stability, which ultimately promotes cancer cell proliferation, migration ...
Li Y, Li S, Li Y, Pan Z.
europepmc   +2 more sources

Mettl3 deficiency leads to the upregulation of Cav1.2 and increases arrhythmia susceptibility in mice

Acta Biochimica et Biophysica Sinica, 2022
Methyltransferase-like 3 (Mettl3) is a component of methyltransferase complex that mediates m6A modification of RNAs, and participates in multiple biological processes. However, the role of Mettl3 in cardiac electrophysiology remains unknown.
Shi Ling, Jin Xuexin, Li Zheng, Gong Rui, Guo Yang, Ma Jiudong, Zhang Yang, Cai Benzhi, Yang Baofeng, Gong Dongmei, Pan Zhenwei   +10 more
doaj   +1 more source