Results 51 to 60 of about 123,166 (168)

MeRIP-seq/scRNA-seq Code

These are the codes for MeRIP-seq/scRNA-seq analysis used in the paper titled "METTL3-Driven RNA Modifications: A Key Mechanism and Potential Therapeutic Target in Pancreatic Acinar Cell Carcinoma in Mice". The relevant GEO accession numbers are as follows: GSE305529, GSE305530.
Tatekawa, Shotaro, Hara, Tomoaki, Meng, Sikun, Sato, Tetsuya, Ishii, Hideshi   +4 more
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m6A Methylation Analysis of Differentially Expressed Genes in Skin Tissues of Coarse and Fine Type Liaoning Cashmere Goats

Frontiers in Genetics, 2020
N6-methyladenosine (m6A) is the most common internal modification in mRNAs of all higher eukaryotes. Here we perform two high-throughput sequencing methods, m6A-modified RNA immunoprecipitation sequence (MeRIP-seq) and RNA sequence (RNA-seq) to identify ...
Yanru Wang, Yuanyuan Zheng, Dan Guo, Xinghui Zhang, Suling Guo, Taiyu Hui, Chang Yue, Jiaming Sun, Suping Guo, Zhixian Bai, Weidong Cai, Xinjiang Zhang, Yixing Fan, Zeying Wang, Wenlin Bai   +14 more
doaj   +1 more source

Methods, Challenges and Potentials of Single Cell RNA-seq [PDF]

, 2012
RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene
Daniel Hebenstreit, Hebenstreit, Daniel
core   +1 more source

Accounting for immunoprecipitation efficiencies in the statistical analysis of ChIP-seq data [PDF]

, 2013
Background: ImmunoPrecipitation (IP) efficiencies may vary largely between different antibodies and between repeated experiments with the same antibody.
Bao, Yanchun,, ’t Hoen, Peter AC, Bao, Yanchun, Veronica Vinciotti, Hoen, P.A.C. 't, Vinciotti, V., Vinciotti, Veronica,, Yanchun Bao, Wit, Ernst,, Bao, Y.C., Ernst Wit, Wit, Ernst, Vinciotti, V, Peter AC ’t Hoen, 't Hoen, Peter A C, Vinciotti, Veronica, Hoen, Peter A.C. ’t,, Bao, Y, 't Hoen, PAC, Wit, E, Wit, E.   +20 more
core   +1 more source

Mettl3 deficiency leads to the upregulation of Cav1.2 and increases arrhythmia susceptibility in mice

Acta Biochimica et Biophysica Sinica, 2022
Methyltransferase-like 3 (Mettl3) is a component of methyltransferase complex that mediates m6A modification of RNAs, and participates in multiple biological processes. However, the role of Mettl3 in cardiac electrophysiology remains unknown.
Shi Ling, Jin Xuexin, Li Zheng, Gong Rui, Guo Yang, Ma Jiudong, Zhang Yang, Cai Benzhi, Yang Baofeng, Gong Dongmei, Pan Zhenwei   +10 more
doaj   +1 more source

MeRIP-seq and RNA-seq data of mycelium and spore of Ascosphaera apis , a widespread bee fungal pathogen [PDF]

, 2020
ABSTRACT Ascosphaera apis is widespread fungal pathogen of honeybee larvae, causing chalkbrood, a chronic disease that weakens bee health and colony productivity. In this article, mecylia and spores of A.
Du, Yu, Jiang, Haibin, Zhu, Zhiwei, Wang, Jie, Chen, Huazhi, Xiong, Cuiling, Zheng, Yanzhen, Chen, Dafu, Guo, Rui   +8 more
openaire   +1 more source

N6-Methyladenosine METTL3 Modulates the Proliferation and Apoptosis of Lens Epithelial Cells in Diabetic Cataract

Molecular Therapy: Nucleic Acids, 2020
N6-methyladenosine (m6A) is the most prevalent eukaryotic messenger RNA modification. Diabetic cataract (DC) is caused by high glucose (HG) in diabetes mellitus. However, the regulatory mechanism of m6A in the DC pathogenesis is poorly understood.
Jun Yang, Jingshu Liu, Shaozhen Zhao, Fang Tian   +3 more
doaj   +1 more source

The Alteration of m6A Modification at the Transcriptome-Wide Level in Human Villi During Spontaneous Abortion in the First Trimester

Frontiers in Genetics, 2022
A growing number of studies have demonstrated that N6 methyladenine (m6A) acts as an important role in the pathogenesis of reproductive diseases. Therefore, it is essential to profile the genome-wide m6A modifications such as in spontaneous abortion.
Jiajie She, Jiajie She, Kaifen Tan, Jie Liu, Shuo Cao, Zengguang Li, You Peng, Zhuoyu Xiao, Ruiying Diao, Liping Wang   +9 more
doaj   +1 more source

Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing

Nature Communications, 2020
Adenovirus transcripts contain N6-methyladenosine (m6A) modification. Here the authors profile m6A modification sites on adenovirus mRNAs using Illumina meRIP-Seq and nanopore direct RNA sequencing, and showcase a role for m6A in splicing of viral late ...
Alexander M. Price, Katharina E. Hayer, Alexa B. R. McIntyre, Nandan S. Gokhale, Jonathan S. Abebe, Ashley N. Della Fera, Christopher E. Mason, Stacy M. Horner, Angus C. Wilson, Daniel P. Depledge, Matthew D. Weitzman   +10 more
doaj   +1 more source

canceromics/MeRIP-simulator: MeRIP-simulator

, 2021
A tool to simulate fasta sequence with m6A ...
canceromics
core   +1 more source