Results 71 to 80 of about 152,412 (174)

A comprehensive evaluation of alignment algorithms in the context of RNA-seq. [PDF]

, 2012
Transcriptome sequencing (RNA-Seq) overcomes limitations of previously used RNA quantification methods and provides one experimental framework for both high-throughput characterization and quantification of transcripts at the nucleotide level.
Friedel, Caroline C., Friedel Caroline C., Caroline C. Friedel (29226), Caroline C Friedel, Lindner, Robert, Lindner Robert, Robert Lindner (107994), Robert Lindner, Caroline C. Friedel   +8 more
core   +2 more sources

Targeting the NR1D1–IGF2BP2–V‐ATPase Axis With Hybrid Nanovesicles Restores Macrophage Rhythms to Reverse Sepsis‐Induced Immunosuppression

Advanced Science, EarlyView.
Sepsis disrupts immune‐cell rhythms and weakens bacterial clearance. Biomimetic nanovesicles combining erythrocyte and inflammation‐activated macrophage membranes deliver siNR1D1 to dysfunctional macrophages, restoring the NR1D1–IGF2BP2–V‐ATPase pathway, circadian regulation, phagolysosomal acidification, and antimicrobial defense.
Lang Chen, Wenyi Lin, Lang Jiang, Xuehui Gao, Chenyan Yu, Jili Han, Zhenping Liu, Xuefeng Li, Mei He, Chang Li, Qilan Li, Chaoying Song, Jiqian Xu, You Shang   +13 more
wiley   +1 more source

Comprehensive analysis of the transcriptome-wide m6A methylome in colorectal cancer by MeRIP sequencing

Epigenetics, 2021
Accumulating evidence has demonstrated that N6-methyladenosine (m6A) plays important roles in various cancers, making it essential to profile m6A modifications at a transcriptome-wide scale in colorectal cancer (CRC).
Zhen Zhang, Quan Wang, Mengmeng Zhang, Wei Zhang, Long Zhao, Changjiang Yang, Bo Wang, Kewei Jiang, Yingjiang Ye, Zhanlong Shen, Shan Wang   +10 more
doaj   +1 more source

ACSL4‐Dependent Lysosomal Lipid Peroxidation Links WTAP‐Mediated m6A Modification to Intervertebral Disc Degeneration

Advanced Science, EarlyView.
IL‐1β upregulates the protein level of WTAP, which promotes the m6A modification of ACSL4 mRNA in an IGF2BP2‐dependent manner, thereby enhancing its stability. The increased ACSL4 drives lipid peroxidation, leading to lysosomal membrane permeabilization (LMP) and impaired mitophagy, which collectively accelerate intervertebral disc degeneration (IVDD).
Shu Jia, Xu Gao, Laimin Zhu, Haixia Song, Jing Wu, Shang Chen, Sheng Gao, Lu Zhang, Chunyang Meng   +8 more
wiley   +1 more source

Comparative analysis of improved m6A sequencing based on antibody optimization for low-input samples

Scientific Reports
The most effective method for mapping N6-methyladenosine (m6A) is m6A RNA immunoprecipitation sequencing (MeRIP-seq). The quality of MeRIP-seq relies on various factors, with the anti-m6A antibody being a crucial determinant.
Jiafeng Lu, Wenjuan Xia, Jincheng Li, Liya Zhang, Chunfeng Qian, Hong Li, Boxian Huang   +6 more
doaj   +1 more source

METTL1-mediated N7-methylguanosine epitranscriptomic alterations modulate mRNA stability of neurodegenerative disease-associated genes following cobalt exposure

Ecotoxicology and Environmental Safety
Excessive cobalt exposure adversely affects the nervous system, yet the underlying neurotoxic mechanisms remain largely elusive. In the present study, using human neuroblastoma H4 cells exposed to cobalt chloride (CoCl₂) as an in vitro model, we ...
Jianping Tang, Yapeng Ren, Jingwen Li, Zhenkun Guo, Guangxia Yu, Fuli Zheng, Wenya Shao, Hong Hu, Siying Wu, Jinfu Zhou, Liangpu Xu, Huangyuan Li   +11 more
doaj   +1 more source

Restoration of m6A‐Modified circPSMB1 Suppresses Chordoma Progression and Enables Local Nanotherapeutic Intervention

Advanced Science, EarlyView.
An m6A‐modified circRNA, circPSMB1, is identified as a tumor suppressor in chordoma. METTL3‐dependent m6A promotes circPSMB1 interaction with HNRNPA2B1, limiting c‐MYC expression and malignant progression. An injectable HMnO2‐based nanohydrogel enables local circPSMB1 delivery and tumor microenvironment‐responsive therapy, offering a promising strategy
Yingchuang Tang, Liang Qiu, Xingbang Ruan, Yihan Shi, Ye Wang, Hanwen Li, Kai Zhang, Kangwu Chen   +7 more
wiley   +1 more source

YTHDC1 Orchestrates Glucose and Glutamate Rewiring to Overcome Lethal Metabolic Stress in Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
Lai et al. find that m6A reader YTHDC1 is specifically overexpressed in triple‐negative breast cancer (TNBC) and orchestrates glucose and glutamine metabolism in an m6A‐dependent manner to confer robust adaptability to lethal metabolic stress. Leveraging YTHDC1 knockdown‐induced metabolic vulnerability, an ATF4 mRNA‐targeted nanotherapy is developed to
Zheng‐Hao Lai, Yi Wang, Na‐Na Li, Ling Zeng, Renzhong Liu, Xue Li, Cheukfai Li, Xin‐Yu Ma, Wenkui Fu, Yingsen Tang, Jinjin Chen, Ning Liao, Man‐Li Luo   +12 more
wiley   +1 more source

MOESM2 of RADAR: differential analysis of MeRIP-seq data with a random effect model

, 2019
Additional file 2. Review history.
Zhang, Zijie, Zhan, Qi, Eckert, Mark, Zhu, Allen, Chryplewicz, Agnieszka, De Jesus, Dario, Ren, Decheng, Kulkarni, Rohit, Lengyel, Ernst, He, Chuan, Chen, Mengjie   +10 more
openaire   +1 more source

Cool Seq Tool

, 2022
What's Changed ci: bump action versions by @jsstevenson in https://github.com/GenomicMedLab/cool-seq-tool/pull/109 refactor!: rename uta tools to cool seq tool by @korikuzma in https://github.com/GenomicMedLab/cool-seq-tool/pull/110 Staging by ...
Kuzma, Kori, Stevenson, James, Wagner, Alex   +2 more
core   +1 more source