Results 211 to 220 of about 6,252 (250)

Synergistic engineering of Casδ nuclease for robust genome editing

Journal of Integrative Plant Biology, EarlyView.
A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.
Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen   +16 more
wiley   +1 more source

The Role of Viruses in the Pathogenesis of Periodontitis

Journal of Periodontal Research, EarlyView.
This narrative review aimed to summarize current evidence on the presence and potential pathogenic role of viruses in periodontitis. Design: Narrative Review; Population: Patients with periodontitis; Exposure: Detection of viruses in oral samples (biopsies, saliva, gingival crevicular fluid, subgingival plaque, blood); Outcome: Viral prevalence, load ...
Kim Natalie Stolte, Jørgen Slots, Henrik Dommisch   +2 more
wiley   +1 more source

Correction: Assessment of Metagenomic Assembly Using Simulated Next Generation Sequencing Data

PLoS ONE, 2014
doaj  

Some of the next articles are maybe not open access.

Related searches:

Neurocysticercosis: diagnosis via metagenomic next-generation sequencing

Practical Neurology, 2023
A 68-year-old Brazilian woman had 3 months of progressive fatigue, difficulty walking and 18 kg weight loss. On examination, there was gait apraxia and executive dysfunction. MR scan of brain showed communicating hydrocephalus and a cerebrospinal fluid showed 105 white cells/µL (≤5), predominantly lymphocytes, protein of 1.35 g/L (0.15–0.45) and the ...
Denison Alves, Pedrosa, Gustavo, Bruniera Peres Fernandes, Luís, Filipe de Souza Godoy, André, C Felício, João Rebello, Pinho, André, Mário Doi, René, de Araújo Gleizer   +6 more
openaire   +2 more sources

Rapid detection of pulmonary nocardiosis by metagenomic next generation sequencing

Diagnostic Microbiology and Infectious Disease, 2023
We retrospectively analyzed 3 cases of pulmonary nocardiosis. Patients were identified by metagenomic next generation sequencing (mNGS) in the First Affiliated Hospital of Soochow University, from January 2019 to April 2022. All patients had underlying diseases, and were in immunocompromised state, with membranous nephropathy, acute lymphoblastic ...
Lirong Gao, Tingting Yang, Xiaohui Zhang, Wei Lei, Jian-an Huang   +4 more
openaire   +2 more sources

Metagenomics Using Next-Generation Sequencing

2014
Traditionally, microbial genome sequencing has been restricted to the small number of species that can be grown in pure culture. The progressive development of culture-independent methods over the last 15 years now allows researchers to sequence microbial communities directly from environmental samples.
Bragg,Lauren, Tyson, Gene W.
openaire   +5 more sources

Adenovirus metagenomic next-generation sequencing

2023
We detected the adenovirus infection in nasopharyngeal swab samples from patients with respiratory tract infection. mNGS was performed on NextSeq 550 and GenoLab M sequencing platforms to study viral phylogenetics and recombination.
openaire   +1 more source

Metagenomic Next-Generation Sequencing in Clinical Microbiology

Clinical Microbiology Newsletter, 2020
Abstract Identification of causative pathogens in infectious disease is a critical component of health care, as infectious diseases continue to be a leading cause of mortality and morbidity worldwide. In addition, the detection of drug resistance by traditional and novel antimicrobial susceptibility testing methods is becoming ever more important as ...
George S. Watts, Bonnie L. Hurwitz
openaire   +1 more source

Metagenomic next-generation sequencing for the diagnosis of neurobrucellosis

Future Microbiology
Objective: This study investigates the application of metagenomic next-generation sequencing (mNGS) in the diagnosis of neurobrucellosis (NB). Methods: We retrospectively analyzed patients diagnosed with NB who underwent cerebrospinal fluid (CSF) mNGS testing in Xijing Hospital from 2015 to 2021. Results: Among the 20 individuals included in the study,
Wen Li, Ying He, Yanyan Li, Xiaona Li, Ting Bian, Tingting Liu, Xuedong Liu, Wen Jiang   +7 more
openaire   +2 more sources

Metagenomic Next-Generation Sequencing of Viruses Infecting Grapevines

2015
Next-generation sequencing (NGS) technologies, for the first time, provide a truly "complete" representation of the viral (and other) pathogens present in a host organism. This is achieved in an unbiased way, and without any prior biological or molecular knowledge of these pathogen(s).
Johan T, Burger, Hans J, Maree
openaire   +2 more sources