The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways [PDF]
Nature Communications, 2018 Microorganisms metabolise methanol using either a methanol methyltransferase or a methanol dehydrogenase. Here, the authors use proteomics and stable isotope fractionation to show that a thermophilic sulfate-reducing bacterium, isolated from the deep ...
Diana Z. Sousa +10 more
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Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant [PDF]
Frontiers in Microbiology, 2021 Methanotrophs have been used to convert methane to methanol at ambient temperature and pressure. In order to accumulate methanol using methanotrophs, methanol dehydrogenase (MDH) must be downregulated as it consumes methanol. Here, we describe a methanol
Hidehiro Ito +5 more
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Discovery and Biochemical Characterization of a Methanol Dehydrogenase From Lysinibacillus xylanilyticus [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Bioconversion of C1 chemicals such as methane and methanol into higher carbon-chain chemicals has been widely studied. Methanol oxidation catalyzed by methanol dehydrogenase (Mdh) is one of the key steps in methanol utilization in bacterial methylotrophy.
Jin-Young Lee +19 more
doaj +2 more sources
Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde [PDF]
Synthetic and Systems Biotechnology, 2023 Methanol is a promising one-carbon feedstock for biomanufacturing, which can be sustainably produced from carbon dioxide and natural gas. However, the efficiency of methanol bioconversion is limited by the poor catalytic properties of nicotinamide ...
Jin Qian +13 more
doaj +2 more sources
Neodymium as Metal Cofactor for Biological Methanol Oxidation: Structure and Kinetics of an XoxF1-Type Methanol Dehydrogenase [PDF]
mBio, 2021 Lanthanides comprise a group of 15 elements with atomic numbers 57 to 71 that are essential in a variety of high-tech devices, such as mobile phones, but were considered biologically inert for a long time.
Rob A. Schmitz +9 more
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The expanded diversity of methylophilaceae from Lake Washington through cultivation and genomic sequencing of novel ecotypes. [PDF]
PLoS ONE, 2014 We describe five novel Methylophilaceae ecotypes from a single ecological niche in Lake Washington, USA, and compare them to three previously described ecotypes, in terms of their phenotype and genome sequence divergence.
David A C Beck +9 more
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MxaY regulates the lanthanide-mediated methanol dehydrogenase switch in Methylomicrobium buryatense [PDF]
PeerJ, 2016 Many methylotrophs, microorganisms that consume carbon compounds lacking carbon–carbon bonds, use two different systems to oxidize methanol for energy production and biomass accumulation.
Frances Chu +2 more
doaj +3 more sources
Methanol Dehydrogenases as a Key Biocatalysts for Synthetic Methylotrophy [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 One-carbon (C1) chemicals are potential building blocks for cheap and sustainable re-sources such as methane, methanol, formaldehyde, formate, carbon monoxide, and more.
Thien-Kim Le +5 more
doaj +3 more sources
Deciphering the assembly process of PQQ dependent methanol dehydrogenase [PDF]
Nature CommunicationsPyrroloquinoline quinone (PQQ)-dependent methanol dehydrogenases (MDHs), the periplasmic metalloenzymes in Gram-negative methylotrophic bacteria, play a pivotal role in methane and methanol bio-utilization.
Haichuan Zhou +16 more
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Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2. [PDF]
ACS Synth Biol, 2019 Synthetic methylotrophy, the modification of organisms such as E. coli to grow on methanol, is a longstanding goal of metabolic engineering and synthetic biology. The poor kinetic properties of NAD-dependent methanol dehydrogenase, the first enzyme in most methanol assimilation pathways, limit pathway flux and present a formidable challenge to ...
Roth TB +3 more
europepmc +5 more sources