Development of Bacillus methanolicus methanol dehydrogenase with improved formaldehyde reduction activity [PDF]
Scientific Reports, 2018 Methanol dehydrogenase (MDH), an NAD+-dependent oxidoreductase, reversibly converts formaldehyde to methanol. This activity is a key step for both toxic formaldehyde elimination and methanol production in bacterial methylotrophy.
Jiyeun Yi +8 more
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Role of Two Methanol Dehydrogenases in Methanol Metabolism of Methylobacterium
Shipin gongye ke-jiIn this study, the pyrroloquinoline quinone (PQQ) high-yield mutant J1-1 of Methylobacterium glucophilum MP688 was used to do bioinformatics analysis to extract the two methanol dehydrogenase (MDH) genes from the genome, mpq0771 and mpq2496.
Yaqing GUO +5 more
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Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy [PDF]
Nature CommunicationsSynthetic methylotrophy offers opportunities for sustainable chemical and biofuel production. While recently established methylotrophic E. coli can grow on methanol, undesirable formate accumulation occurs during growth and bioproduction.
Philipp Keller +10 more
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A catalytic role of XoxF1 as La3+-dependent methanol dehydrogenase in Methylobacterium extorquens strain AM1. [PDF]
PLoS ONE, 2012 In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca(2+)-dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol.
Tomoyuki Nakagawa +7 more
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Synthetic C1 metabolism in Pseudomonas putida enables strict formatotrophy and methylotrophy via the reductive glycine pathway [PDF]
mBioFormate and methanol are promising alternatives to sugar-based feedstocks for biotechnological applications. These one-carbon (C1) substrates can be sustainably produced from CO2 and renewable electricity and assimilated by both native and engineered ...
Justine Turlin +5 more
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Oligotrophic Gene Expression in Rhodococcus erythropolis N9T-4 under Various Nutrient Conditions
Microorganisms, 2022 Rhodococcus erythropolis N9T-4 is a super oligotroph that grows on an inorganic basal medium without any additional carbon and nitrogen sources and requires CO2 for its oligotrophic growth. Previously, we found that two genes, aldA and mnoA, encoding NAD-
Yuri Ikeda +3 more
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Mechanism of methanol oxidation by quinoprotein methanol dehydrogenase [PDF]
Proceedings of the National Academy of Sciences, 2007 At neutral pH, oxidation of CH 3 OH → CH 2 O by an o -quinone requires general-base catalysis and the reaction is endothermic. The active-site –CO 2 − groups of Glu-171 and Asp-297 (Glu-171–CO 2 ...
Xiaodong, Zhang +2 more
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PLoS ONE, 2021 Methanol is often considered as a non-competitive substrate for methanogenic archaea, but an increasing number of sulfate-reducing microorganisms (SRMs) have been reported to be capable of respiring with methanol as an electron donor.
Min Sub Sim +2 more
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Hayati Journal of Biosciences, 2011 The human feet and mouth are known as sources of methylated sulfides, which are produced by other microflora. Methylated sulfides could be oxidized by methylotrophic bacteria, which may result in odor reduction in human feet and mouth.
DIANA ELIZABETH WATURANGI +3 more
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A Periplasmic Lanthanide Mediator, Lanmodulin, in Methylobacterium aquaticum Strain 22A
Frontiers in Microbiology, 2022 Methylobacterium and Methylorubrum species oxidize methanol via pyrroloquinoline quinone-methanol dehydrogenases (MDHs). MDHs can be classified into two major groups, Ca2+-dependent MDH (MxaF) and lanthanide (Ln3+)-dependent MDH (XoxF), whose expression ...
Yoshiko Fujitani +2 more
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