Microbial Biotechnology, 2019 Summary Pichia pastoris KM71H (MutS) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP‐powered efflux pump which is expressed properly, but at very low concentration, using the conventional ...
Wan‐cang Liu +3 more
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Trace metal requirements for microbial enzymes involved in the production and consumption of methane and nitrous oxide [PDF]
, 2012 Fluxes of greenhouse gases to the atmosphere are heavily influenced by microbiological activity. Microbial enzymes involved in the production and consumption of greenhouse gases often contain metal cofactors.
Glass, Jennifer B., Orphan, Victoria J.
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"A Methylotrophic Thermotolerant Actinomycete With a NAD+- Dependent Methanol Dehydrogenase" [PDF]
Iranian Journal of Public Health, 2002 The methylotrophic thermo tolerant actinomycete strain 381, was isolated from a compost sample, assimilated methanol during growth. Methanol dehydrogenase could not be detected in cell extracts using dye-linked assays in the absence of NAD ...
S Eshraghi, Ian C Hancock, E Williams
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Novel methylotrophic bacteria isolated from the River Thames (London, UK) [PDF]
, 2008 Enrichment and elective culture for methylotrophs from sediment of the River Thames in central London yielded a diversity of pure cultures representing several genera of Gram-negative and Gram-positive bacteria, which were mainly of organisms not ...
Alves +75 more
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Evidence of the indirect hormonal activity of prohormones using liver S9 metabolic bioactivation and an androgen bioassay [PDF]
, 2008 Prohormones such as dehydroepiandrosterone (DHEA) are steroid precursors that do not show hormonal activity by themselves. Abuse of these prohormones in cattle fattening is hard to prove because of strong in vivo metabolism and the difficulty to detect ...
Bovee, T.F.H. +4 more
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Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties. [PDF]
PLoS ONE, 2013 Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD(+)-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of the B.
Anne Krog +7 more
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Assessing Lanthanide-Dependent Methanol Dehydrogenase Activity: The Assay Matters
ChemBioChem, 2023 AbstractArtificial dye-coupled assays have been widely adopted as a rapid and convenient method to assess the activity of methanol dehydrogenases (MDH). Lanthanide(Ln)-dependent XoxF-MDHs from methanotrophs and methylotrophs are able to incorporate different lanthanides (Lns) in their active site.
Manh Tri Phi +6 more
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Lanthanide-Dependent Regulation of Methylotrophy in
mSphere, 2018 Methylobacterium species are representative of methylotrophic bacteria. Their genomes usually encode two types of methanol dehydrogenases (MDHs): MxaF and XoxF.
Sachiko Masuda +6 more
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Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol
Bioengineering, 2023 The naturally occurring one-carbon assimilation pathways for the production of acetyl-CoA and its derivatives often have low product yields because of carbon loss as CO2.
Jiaying Wang +4 more
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Microbial Oxidation of Methane and Methanol: Crystallization of Methanol Dehydrogenase and Properties of Holo- and Apo-Methanol Dehydrogenase from Methylomonas methanica [PDF]
Journal of Bacteriology, 1978 Procedures are described for the purification and crystallization of methanol dehydrogenase from the soluble fraction of the type I obligate methylotroph Methylomonas methanica strain S1. The crystallized enzyme is homogeneous as judged by acrylamide gel electrophoresis and ultracentrifugation. The enzyme had a high
R N, Patel, C T, Hou, A, Felix
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