Results 121 to 130 of about 169 (167)
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2011
DNA methyltransferases are important enzymes and their inhibition has many potential applications. The investigation of DNA methyltransferases as well as screening for potential inhibitors requires specialized enzyme assays. In this chapter, we describe three DNA methyltransferase assays, each of them based on a different method: (1) An assay using ...
Renata Z, Jurkowska +4 more
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DNA methyltransferases are important enzymes and their inhibition has many potential applications. The investigation of DNA methyltransferases as well as screening for potential inhibitors requires specialized enzyme assays. In this chapter, we describe three DNA methyltransferase assays, each of them based on a different method: (1) An assay using ...
Renata Z, Jurkowska +4 more
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Microassay for DNA methyltransferase
Journal of Biochemical and Biophysical Methods, 1991A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters.
R L, Adams, A, Rinaldi, C, Seivwright
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Pharmacogenomics: Catechol O-Methyltransferase to Thiopurine S-Methyltransferase
Cellular and Molecular Neurobiology, 20061. Pharmacogenomics is the study of the role of inheritance in variation in the drug response phenotype-a phenotype that can vary from adverse drug reactions at one end of the spectrum to lack of therapeutic efficacy at the other. 2. The thiopurine S-methyltransferase (TPMT) genetic polymorphism represents one of the best characterized and most ...
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Histone methyltransferases in cancer
Seminars in Cell & Developmental Biology, 2010Cancer is perceived as a heterogeneous group of diseases that is characterized by aberrant patterns of gene expression. In the last decade, an increasing amount of data has pointed to a key role for epigenetic alterations in human cancer. In this review, we focus on a subclass of epigenetic regulators, namely histone methyltransferases (HMTs).
Albert, Mareike, Helin, Kristian
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Immobilization of S-Methyltransferase
Enzyme, 2017S-methyltransferase was solubilized from pig liver microsomes by treatment with N-dodecyl-N,N-dimethyl-3-ammonio-l-sulfonate (Zwittergent®). The soluble enzyme was immobilized by covalent binding to agarose and by copolymerization with acrylamide. The specific activity for the agarose-bound enzyme towards the substrate ethane thiol was 0.87 nmol/min/mg
G, Brunner, F, Tegtmeier
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Plant Molecular Biology, 2000
DNA methylation is an important modification of DNA that plays a role in genome management and in regulating gene expression during development. Methylation is carried out by DNA methyltransferases which catalyse the transfer of a methyl group to bases within the DNA helix.
E J, Finnegan, K A, Kovac
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DNA methylation is an important modification of DNA that plays a role in genome management and in regulating gene expression during development. Methylation is carried out by DNA methyltransferases which catalyse the transfer of a methyl group to bases within the DNA helix.
E J, Finnegan, K A, Kovac
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Elongation factor methyltransferase 3 – A novel eukaryotic lysine methyltransferase
Biochemical and Biophysical Research Communications, 2014Here we describe the discovery of Saccharomycescerevisiae protein YJR129Cp as a new eukaryotic seven-beta-strand lysine methyltransferase. An immunoblotting screen of 21 putative methyltransferases showed a loss in the methylation of elongation factor 2 (EF2) on knockout of YJR129C.
Lelin, Zhang +4 more
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Cobalamin-Dependent Methyltransferases
Accounts of Chemical Research, 2001Cobalamin cofactors play critical roles in radical-catalyzed rearrangements and in methyl transfers. This Account focuses on the role of methylcobalamin and its structural homologues, the methylcorrinoids, as intermediaries in methyl transfer reactions, and particularly on the reaction catalyzed by cobalamin-dependent methionine synthase.
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Lysine Methyltransferases and Their Inhibitors
2019Since 2000, the histone methyltransferases that catalyze the methylation of a number of histone and nonhistone substrates have been discovered.
Stazi, Giulia +2 more
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