Results 211 to 220 of about 217,730 (262)
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Methyltransferase DNMT3B in leukemia
Leukemia & Lymphoma, 2019DNA methyltransferases (DNMTs) are highly conserved DNA-modifying enzymes that play important roles in epigenetic regulation and they are involved in cell proliferation, differentiation, and apoptosis. In mammalian cells, three active DNMTs have been identified: DNMT1 acts as a maintenance methyltransferase to replicate preexisting methylation patterns,
Haibin, Zhang +2 more
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EUKARYOTIC CYTOSINE METHYLTRANSFERASES
Annual Review of Biochemistry, 2005▪ Abstract Large-genome eukaryotes use heritable cytosine methylation to silence promoters, especially those associated with transposons and imprinted genes. Cytosine methylation does not reinforce or replace ancestral gene regulation pathways but instead endows methylated genomes with the ability to repress specific promoters in a manner that is ...
Mary Grace, Goll, Timothy H, Bestor
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Biochimica et Biophysica Acta (BBA) - Enzymology, 1968
An enzyme that transfers the methyl group of S-adenosylmethionine to phenol to form anisole is described. The enzyme is highly localized in the microsomes of the liver and lung of mammals and is also present in other tissues. A variety of simple alkyl-, methoxy- and halophenols can serve as substrates for the phenol-O-methylating enzyme.
J, Axelrod, J, Daly
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An enzyme that transfers the methyl group of S-adenosylmethionine to phenol to form anisole is described. The enzyme is highly localized in the microsomes of the liver and lung of mammals and is also present in other tissues. A variety of simple alkyl-, methoxy- and halophenols can serve as substrates for the phenol-O-methylating enzyme.
J, Axelrod, J, Daly
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The phospholipid methyltransferases in yeast
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1997In fungal microorganisms including fission yeast, Schizosaccharomyces pombe and baker's yeast, Saccharomyces cerevisiae, two enzymes are required to catalyze the synthesis of phosphatidylcholine (PC) from phosphatidylethanolamine (PE). The genes encoding the class I and class II phospholipid N-methyltransferases (PLMTs) have been cloned from both ...
M I, Kanipes, S A, Henry
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2011
DNA methyltransferases are important enzymes and their inhibition has many potential applications. The investigation of DNA methyltransferases as well as screening for potential inhibitors requires specialized enzyme assays. In this chapter, we describe three DNA methyltransferase assays, each of them based on a different method: (1) An assay using ...
Renata Z, Jurkowska +4 more
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DNA methyltransferases are important enzymes and their inhibition has many potential applications. The investigation of DNA methyltransferases as well as screening for potential inhibitors requires specialized enzyme assays. In this chapter, we describe three DNA methyltransferase assays, each of them based on a different method: (1) An assay using ...
Renata Z, Jurkowska +4 more
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Pharmacogenomics: Catechol O-Methyltransferase to Thiopurine S-Methyltransferase
Cellular and Molecular Neurobiology, 20061. Pharmacogenomics is the study of the role of inheritance in variation in the drug response phenotype-a phenotype that can vary from adverse drug reactions at one end of the spectrum to lack of therapeutic efficacy at the other. 2. The thiopurine S-methyltransferase (TPMT) genetic polymorphism represents one of the best characterized and most ...
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Microassay for DNA methyltransferase
Journal of Biochemical and Biophysical Methods, 1991A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters.
R L, Adams, A, Rinaldi, C, Seivwright
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Histone methyltransferases in cancer
Seminars in Cell & Developmental Biology, 2010Cancer is perceived as a heterogeneous group of diseases that is characterized by aberrant patterns of gene expression. In the last decade, an increasing amount of data has pointed to a key role for epigenetic alterations in human cancer. In this review, we focus on a subclass of epigenetic regulators, namely histone methyltransferases (HMTs).
Albert, Mareike, Helin, Kristian
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Immobilization of S-Methyltransferase
Enzyme, 2017S-methyltransferase was solubilized from pig liver microsomes by treatment with N-dodecyl-N,N-dimethyl-3-ammonio-l-sulfonate (Zwittergent®). The soluble enzyme was immobilized by covalent binding to agarose and by copolymerization with acrylamide. The specific activity for the agarose-bound enzyme towards the substrate ethane thiol was 0.87 nmol/min/mg
G, Brunner, F, Tegtmeier
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