Results 91 to 100 of about 1,517 (162)

Alternative approach to deplete HIF1α impairs MHV68 replication.

, 2019
(A) 3T12 cells were treated with a pool of siHIF1α or siNT (non-targeting) for 24 hours. Top left panel: HIF1α protein expression at 24hpi of uninfected and MHV68 (MOI:3) infected cells at 3%O2. Beta-actin was probed for loading control. Top middle panel:
Darlah M. López-Rodríguez (8098544), Varvara Kirillov (8098547), Enrique A. Mesri (515944), Laurie T. Krug (8098550), Samita Andreansky (3401330)   +4 more
core   +1 more source

Number of host mRNA hybrid sequences containing MHV68 miRNAs.

, 2019
For each hybrid containing a host mRNA, the total number of sequences containing each of the 28 potential mature MHV68 miRNAs was enumerated. For each sample type (latency, reactivation, lytic) and corresponding miRNA, numbers represent the total unique ...
Yiping Wang (1484218), Rolf Renne (30420), Whitney L. Bullard (7163573), Scott A. Tibbetts (7163588), Mehmet Kara (4814757), April Feswick (241846), Brett A. Hoffman (7163585), Lauren A. Gay (7163576), Shreya Nirmalan (7163579), Alim Esemenli (7163582), Sunantha Sethuraman (4253008)   +10 more
core   +1 more source

Enhanced response of T cells from murine gammaherpesvirus 68-infected mice lacking the suppressor of T cell receptor signaling molecules Sts-1 and Sts-2.

PLoS ONE, 2014
The human gammaherpesviruses establish life-long infections that are associated with the development of lymphomas and neoplasms, especially in immunocompromised individuals. T cells play a crucial role in the control of gammaherpesvirus infection through
Brandon Cieniewicz, Nicholas Carpino, Laurie T Krug   +2 more
doaj   +1 more source

Thirty novel miRNAs in the MHV68 or mouse genomes.

, 2012
aThe number of reads perfectly mapping to pre-miRNA hairpin sequences in all samples.bThe rank of miRNAs relative to all known and novel murine miRNAs.*The rank of miRNAs relative to all the MHV68 miRNAs.
Weixiong Zhang (10203), Jing Xia (124148)   +1 more
core   +1 more source

Murine gammaherpesvirus infection is skewed toward Igλ+ B cells expressing a specific heavy chain V-segment.

PLoS Pathogens, 2020
One of the defining characteristics of the B cell receptor (BCR) is the extensive diversity in the repertoire of immunoglobulin genes that make up the BCR, resulting in broad range of specificity.
Christopher M Collins, Christopher D Scharer, Thomas J Murphy, Jeremy M Boss, Samuel H Speck   +4 more
doaj   +1 more source

mLANA mutants that have lost the capacity to repress transcription from the MHV68 TR-driven luciferase reporter construct (MHV68-73TRN mutants) display similar growth kinetics and virus output as the mLANA null mutant virus (73.Stop virus).

, 2013
Six mLANA mutants were introduced into the MHV68 genome using BAC recombineering. (A) After infection of NIH3T3 fibroblasts with each MHV68-73TRN virus at an MOI of 3 PFU/mL, cell lysates were harvested at one and seven hours post-infection.
Samuel H. Speck (70359), Clinton R. Paden (206576), Scott A. Tibbetts (244898), J. Craig Forrest (308744)   +3 more
core   +1 more source

RTA Occupancy of the Origin of Lytic Replication during Murine Gammaherpesvirus 68 Reactivation from B Cell Latency

Pathogens, 2017
RTA, the viral Replication and Transcription Activator, is essential for rhadinovirus lytic gene expression upon de novo infection and reactivation from latency.
Alexis L. Santana, Darby G. Oldenburg, Varvara Kirillov, Laraib Malik, Qiwen Dong, Roman Sinayev, Kenneth B. Marcu, Douglas W. White, Laurie T. Krug   +8 more
doaj   +1 more source

KSHV K-cyclin does not rescue MHV68 v-cyclin null virus reactivation from splenocytes following intranasal inoculation.

, 2014
C57Bl/6 mice were infected via intranasal inoculation with 1,000 pfu of either WT MHV68, vCyclin.stop or a recombinant MHV68 expressing the KSHV v-cyclin from the MHV68 v-cyclin locus (K-cyclin). (A) Reactivation from splenocytes on day 18 post infection
Francine M. Scott (546179), Samuel H. Speck (70359)   +1 more
core   +1 more source

Long term marking of splenocytes infected with MHV68-H2bYFP.

, 2013
YFP+ and YFP− splenocytes from mice were sorted at 42 days post-infection from mice infected with 1,000 pfu of MHV68-H2bYFP. Limiting dilution PCR analysis was performed to determine the frequency of viral genome positive cells in each fraction.
Samuel H. Speck (70359), Christopher M. Collins (309503)   +1 more
core   +1 more source

Pol II subunits are depleted in the nucleus of MHV68 infected cells in an RNA decay dependent manner.

, 2020
(A) Diagram showing the experimental setup for the TMT-MS. (B) Graphs showing the nuclear distribution of poly(A) binding proteins from the TMT-MS data. Graphs display the mean with SEM of 3 biological replicates.
Ileana M. Cristea (246789), Britt A. Glaunsinger (8423214), Joel D. Federspiel (3368378), Sarah Gilbertson (8423211), Ella Hartenian (8423208)   +4 more
core   +1 more source