Differential Signaling Pathways Identified in Aqueous Humor, Anterior Capsule, and Crystalline Lens of Age-Related, Diabetic, and Post-Vitrectomy Cataract [PDF]
ProteomesBackground: The purpose of this study was to detect proteomic alterations and corresponding signaling pathways involved in the formation of age-related cataract (ARC), diabetic cataract (DC), and post-vitrectomy cataract (PVC).
Christina Karakosta +6 more
doaj +2 more sources
Journal of Cell Science, 1991 ABSTRACT Microinjection of specific antibodies can be an alternative and a supplement to genetic engineering in dissecting the function of individual cytoskeletal components. In this report, we describe some of the requirements for using this technique, its potential application in conjunction with morphological and biochemical analyses,
B. Jockusch +4 more
semanticscholar +3 more sources
Anticytoskeletal autoantibody to microfilament anchorage sites recognizes novel focal contact proteins. [PDF]
Journal of Clinical Investigation, 1987 Actin microfilaments are anchored to the plasma membrane at focal contacts. Using an indirect immunofluorescence method, we detected an autoantibody reactive with focal contacts in PtK2, HEp-2, and BHK-21 cells in serum from two patients with early ...
J. Senécal +3 more
semanticscholar +4 more sources
A marginal band-associated protein has properties of both microtubule- and microfilament-associated proteins [PDF]
The Journal of cell biology, 1989 The marginal band of nucleated erythrocytes is a microtubule organelle under rigorous quantitative and spatial control, with properties quite different from those of the microtubule organelles of cultured cells.
Eric Birgbauer, Frank Solomon
semanticscholar +4 more sources
Profilin and actin-related proteins regulate microfilament dynamics during early mammalian embryogenesis. [PDF]
Human Reproduction, 2006 BACKGROUND Profilins are ubiquitous proteins widely distributed in animals, including humans. They regulate actin polymerization by sequestering actin monomers in association with other actin-related proteins (Arps).
V. Rawe, C. Payne, G. Schatten
semanticscholar +4 more sources
Temporal Profiling of Cellular and Molecular Processes in Osteodifferentiation of Dental Pulp Stem Cells [PDF]
BiologyBased on the potential of DPSCs as the most promising candidates for bone tissue engineering, we comprehensively investigated the time-dependent cellular and molecular changes that occur during their osteodifferentiation.
Bibiána Baďurová +8 more
doaj +2 more sources
Journal of Cell Science, 1986 ABSTRACT Fluorescently labelled contractile proteins (alpha-actinin and filamin) were used to study the dynamic nature of three types of microfilament bundles: myofibrils, stress fibres and polygonal networks. Cultured muscle and non-muscle cells that were microinjected with fluorescent alphaactinin rapidly incorporated the labelled ...
J. Sanger +3 more
semanticscholar +4 more sources
Biomolecules, 2021 Human skin fibroblasts (HSFs) approximate the multidirectional differentiation potential of mesenchymal stem cells, so they are often used in differentiation, cell cultures, and injury repair. They are an important seed source in the field of bone tissue
Xiaolan Huang +12 more
doaj +1 more source
Flow-Induced Buckling of Elastic Microfilaments with Non-Uniform Bending Stiffness [PDF]
Front. Soft Matter (2023) 2:977729, 2022 Buckling plays a critical role in the transport and dynamics of elastic microfilaments in Stokesian fluids. However, previous work has only considered filaments with homogeneous structural properties. Filament backbone stiffness can be non-uniform in many biological systems like microtubules, where the association and disassociation of proteins can ...
arxiv +1 more source
Signaling via ITGB1/FAK and microfilament rearrangement mediates the internalization of Leptospira interrogans in mouse J774A.1 macrophages [PDF]
Archives of Biological Sciences, 2015 Leptospirosis caused by pathogenic Leptospira species is a worldwide zoonotic 2 infectious disease, but the mechanisms of leptospiral internalization remain poorly understood.
Zhao Xin +4 more
doaj +1 more source