Results 11 to 20 of about 25,497 (250)
Gas2, a growth arrest-specific protein, is a component of the microfilament network system [PDF]
The Journal of cell biology, 1992 In this report we analyze the protein product of a growth arrest-specific gene, gas2, by means of an affinity-purified antibody raised against the protein produced in bacteria. The regulation of Gas2 biosynthesis reflects the pattern of mRNA expression (Schneider, C., R. King, and L. Philipson. 1988. Cell.
Claudio Brancolini +2 more
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Molecular imaging of membrane proteins and microfilaments using atomic force microscopy [PDF]
Experimental and Molecular Medicine, 2010 Atomic force microscopy (AFM) is an emerging technique for a variety of uses involving the analysis of cells. AFM is widely applied to obtain information about both cellular structural and subcellular events. In particular, a variety of investigations into membrane proteins and microfilaments were performed with AFM.
Se‐Hui Jung +4 more
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Anticytoskeletal autoantibody to microfilament anchorage sites recognizes novel focal contact proteins. [PDF]
Journal of Clinical Investigation, 1987 Actin microfilaments are anchored to the plasma membrane at focal contacts. Using an indirect immunofluorescence method, we detected an autoantibody reactive with focal contacts in PtK2, HEp-2, and BHK-21 cells in serum from two patients with early systemic sclerosis. With double immunofluorescence, using the actin-binding drug phalloidin, we localized
Jean‐Luc Senécal +3 more
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Basolateral Membrane-associated 27-kDa Heat Shock Protein and Microfilament Polymerization [PDF]
Journal of Biological Chemistry, 1997 The in vivo activity of the 27-kDa heat shock protein, a barbed-end microfilament capping protein, may be localized to the plasma membrane. To investigate this putative association, bovine endothelial cells expressing the human wild type or a mutant nonphosphorylatable 27-kDa heat shock protein were subjected to subcellular fractionation and immunoblot
Randolph S. Piotrowicz, Eugene G. Levin
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An Essential Cytoskeletal Linker Protein Connecting Actin Microfilaments to Intermediate Filaments [PDF]
Cell, 1996 Typified by rapid degeneration of sensory neurons, dystonia musculorum mice have a defective BPAG1 gene, known to be expressed in epidermis. We report a neuronal splice form, BPAG1n, which localizes to sensory axons. Both isoforms have a coiled-coil rod, followed by a carboxy domain that associates with intermediate filaments.
Yanmin Yang +5 more
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The Roles of Microfilament-Associated Proteins, Drebrins, in Brain Morphogenesis: A Review [PDF]
Journal of Biochemistry, 1995 The cytoskeleton has been suggested to be one of the important endogenous factors that control neuronal morphogenesis. Analysis of the developmental changes in the protein composition of the brain led to the discovery of novel developmentally regulated actin-binding proteins, drebrins.
Tomoaki Shirao
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Profilin and actin-related proteins regulate microfilament dynamics during early mammalian embryogenesis [PDF]
Human Reproduction, 2006 Profilins are ubiquitous proteins widely distributed in animals, including humans. They regulate actin polymerization by sequestering actin monomers in association with other actin-related proteins (Arps). Actin remodelling is essential for oocyte maturation, fertilization and embryo development; yet the role of profilins in these events is not well ...
Vanesa Y. Rawe +2 more
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Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins [PDF]
The Journal of Cell Biology, 2001 By screening a yeast two-hybrid library with COOH-terminal fragments of vinculin/metavinculin as the bait, we identified a new protein termed raver1. Raver1 is an 80-kD multidomain protein and widely expressed but to varying amounts in different cell lines. In situ and in vitro, raver1 forms complexes with the microfilament-associated proteins vinculin,
Stefan Hüttelmaier +5 more
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Modulation of microfilament protein composition by transfected cytoskeletal actin genes. [PDF]
Molecular and Cellular Biology, 1988 HuT-14T is a highly tumorigenic fibroblast cell line which exhibits a reduced steady-state level of beta-actin due to coding mutations in one of two beta-actin alleles. The normal rate of total actin synthesis could be restored in some clones of cells following transfection of the functional beta-actin gene but not following transfection of the ...
S Y Ng +4 more
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