Molecular imaging of membrane proteins and microfilaments using atomic force microscopy [PDF]
Atomic force microscopy (AFM) is an emerging technique for a variety of uses involving the analysis of cells. AFM is widely applied to obtain information about both cellular structural and subcellular events. In particular, a variety of investigations into membrane proteins and microfilaments were performed with AFM.
Jae Hyo Park+4 more
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The human mutant cardiac α-actins p.A295S or p.R312H and p.E361G, correlated with hypertrophic or dilated cardiomyopathy, respectively, were expressed by the baculovirus/Sf21 insect cell system and purified to homogeneity.
Constanze Erdmann+10 more
doaj +1 more source
Chemical regulators of epithelial plasticity reveal a nuclear receptor pathway controlling myofibroblast differentiation [PDF]
Plasticity in epithelial tissues relates to processes of embryonic development, tissue fibrosis and cancer progression. Pharmacological modulation of epithelial transitions during disease progression may thus be clinically useful.
Bellomo, C+12 more
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Vitronectin at sites of cell-substrate contact in cultures of rat myotubes [PDF]
Affinity-purified antibodies to the serum glycoprotein, vitronectin, were used to study sites of cell-substrate contact in cultures of rat myotubes and fibroblasts.
Baetscher, Manfred+2 more
core +1 more source
Intermediate filament–membrane attachments function synergistically with actin-dependent contacts to regulate intercellular adhesive strength [PDF]
By tethering intermediate filaments (IFs) to sites of intercellular adhesion, desmosomes facilitate formation of a supercellular scaffold that imparts mechanical strength to a tissue.
Amargo, Evangeline V.+11 more
core +4 more sources
The exocyst at the interface between cytoskeleton and membranes in eukaryotic cells
Delivery and final fusion of the secretory vesicles with the relevant target membrane are hierarchically organized and reciprocally interconnected multi-step processes involving not only specific protein-protein interactions, but also specific protein ...
Lukas eSynek+4 more
doaj +1 more source
Actin Cytoskeleton and Golgi Involvement in Barley stripe mosaic virus Movement and Cell Wall Localization of Triple Gene Block Proteins. [PDF]
Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene ...
Bae, Hanhong+10 more
core +2 more sources
Synthesis of proto-oncogene proteins and cyclins depends on intact microfilaments
It is well established that microfilament disintegration by cytochalasin D (CD) as well as latrunculin (LAT)-A and LAT-B causes an inhibition of S phase entry of various nontransformed cell lines. Our experiments extended these observations to human embryonal diploid fibroblasts (Wi-38). To investigate the question whether this stop of DNA synthesis is
Martin Iwig+2 more
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The Arabidopsis thaliana Class II Formin FH13 Modulates Pollen Tube Growth
Formins are a large, evolutionarily conserved family of actin-nucleating proteins with additional roles in regulating microfilament, microtubule, and membrane dynamics.
Eva Kollárová+2 more
doaj +1 more source
HIV-1 Gag Protein Associates with F-actin Present in Microfilaments
Several studies have provided evidence that the cellular cytoskeleton may be involved in the assembly and budding of retroviruses. In fractionation studies of HIV-1-infected CEM cells, the majority of the unprocessed Gag polyprotein cofractionated with the cellular cytoskeleton.
Jude Canon, Osvaldo Rey, Paul Krogstad
openaire +3 more sources