Results 71 to 80 of about 97,218 (302)

Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems

open access: yesFrontiers in Pharmacology, 2019
Cell-free protein synthesis (CFPS) based on eukaryotic Sf21 lysate is gaining interest among researchers due to its ability to handle the synthesis of complex human membrane proteins (MPs).
Srujan Kumar Dondapati   +6 more
doaj   +1 more source

Protein retention in yeast rough endoplasmic reticulum: expression and assembly of human ribophorin I. [PDF]

open access: yes, 1990
The RER retains a specific subset of ER proteins, many of which have been shown to participate in the translocation of nascent secretory and membrane proteins. The mechanism of retention of RER specific membrane proteins is unknown.
Crowe, JS, Meyer, DI, Sanderson, CM
core  

Kinetic Studies on the Transport of Cytoplasmically Synthesized Proteins into the Mitochondria in Intact Cells of Neurospora crassa [PDF]

open access: yes, 1977
The transport of cytoplasmically synthesized mitochondrial proteins was investigated in whole cells of Neurospora crassa, using dual labelling and immunological techniques.
Hallermayer, Gerhard   +2 more
core   +1 more source

Biosynthesis and Identification of Clenbuterol Metabolites in Urine and In Vitro Microsome Incubation Samples Using UHPLC‐Q‐Exactive Orbitrap Mass Spectrometry: A Comparison Between Human and Bovine Metabolism

open access: yesDrug Testing and Analysis, EarlyView.
Clenbuterol metabolism was studied in bovine and human urine using LC‐Q‐Exactive‐Orbitrap MS; eight metabolites were identified in human and bovine samples as well as a novel N‐methylated form. Four urinary metabolites specific to cattle were detected and identified.
Anuar Gómez‐Tagle   +5 more
wiley   +1 more source

Revisiting the Latency of Uridine Diphosphate-Glucuronosyltransferases (UGTs)—How Does the Endoplasmic Reticulum Membrane Influence Their Function?

open access: yesPharmaceutics, 2017
Uridine diphosphate-glucuronosyltransferases (UGTs) are phase 2 conjugation enzymes mainly located in the endoplasmic reticulum (ER) of the liver and many other tissues, and can be recovered in artificial ER membrane preparations (microsomes).
Yuejian Liu, Michael W. H. Coughtrie
doaj   +1 more source

Molecular biomarkers and toxic consequences of impact by organic pollution in aquatic organisms [PDF]

open access: yes, 1994
Organic contaminants are readily bioaccumulated by aquatic organisms. Exposure to and toxic effects of contaminants can be measured in terms of the biochemical responses of the organisms (i.e. molecular biomarkers).
Förlin, L.   +2 more
core  

An ancestral secretory apparatus in the protozoan parasite Giardia intestinalis [PDF]

open access: yes, 2003
The protozoan parasite Giardia intestinalis belongs to one of the earliest diverged eukaryotic lineages. This is also reflected in a simple intracellular organization, as Giardia lacks common subcellular compartments such as mitochondria, peroxisomes ...
Hehl, Adrian B.   +7 more
core   +1 more source

Interpretation of in vitro concentration‐response data for risk assessment and regulatory decision‐making: Report from the 2022 IWGT quantitative analysis expert working group meeting

open access: yesEnvironmental and Molecular Mutagenesis, EarlyView.
Abstract Quantitative risk assessments of chemicals are routinely performed using in vivo data from rodents; however, there is growing recognition that non‐animal approaches can be human‐relevant alternatives. There is an urgent need to build confidence in non‐animal alternatives given the international support to reduce the use of animals in toxicity ...
Marc A. Beal   +14 more
wiley   +1 more source

Structural analysis of phosphatidylcholine of plant tissue

open access: yesJournal of Lipid Research, 1971
Pure preparations of phosphatidylcholine were isolated from spinach leaf chloroplasts, spinach leaf microsomes, and cauliflower inflorescence. The isolated phosphatidylcholine was treated with snake venom phospholipase A, and the fatty acid distribution ...
K.A. DEVOR, J.B. MUDD
doaj   +1 more source

Development of indole sulfonamides as cannabinoid receptor negative allosteric modulators [PDF]

open access: yes, 2016
This Letter was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) and the Scottish Universities Life Sciences Alliance (SULSA) in 2011Peer ...
Abdelrahman, Mostafa Hamed   +4 more
core   +1 more source

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