Results 151 to 160 of about 335,784 (179)

Augmin complex activity finetunes dendrite morphology through non-centrosomal microtubule nucleation in vivo.

J Cell Sci
Zhang Y, Sung HH, Ziegler AB, Wu YC, Viais R, Sánchez-Huertas C, Kilo L, Agircan FG, Cheng YJ, Mouri K, Uemura T, Lüders J, Chien CT, Tavosanis G.   +13 more
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Microtubule nucleation: beyond the template

Nature Reviews Molecular Cell Biology, 2017
Microtubules are cytoskeletal filaments central to a wide range of essential cellular functions in eukaryotic cells. Consequently, cells need to exert tight control over when, where and how many microtubules are being made. Whereas the regulation of microtubule dynamics is well studied, the molecular mechanisms of microtubule nucleation are still ...
Thomas Surrey, Johanna Roostalu, Surrey Thomas   +2 more
exaly   +4 more sources

Microtubule-dependent microtubule nucleation in plant cells

Journal of Plant Research, 2006
Regulation of microtubule nucleation sites is an essential step in microtubule organization. Cortical microtubule arrays in green plant cells at inter-phase are organized in a distinct manner--the array is formed in the absence of previously recognized organelles for microtubule nucleation, for example the centrosome and spindle pole body. Microtubules
Mitsuyasu Hasebe, Takashi Murata, Hasebe Mitsuyasu   +2 more
exaly   +3 more sources

Assaying Microtubule Nucleation.

Methods in molecular biology, 2020
Assaying microtubule nucleation is essential to understand the organization of microtubule networks in any cell type. In this chapter we describe methods for measuring nucleation activity at centrosomes and at mitotic chromatin in cell lines, to study interphase and mitotic microtubule organization, and for measuring non-centrosomal nucleation in ...
Artur Ezquerra, Ricardo Viais, Jens Lüders   +2 more
semanticscholar   +3 more sources

Microtubule nucleation

Current Opinion in Cell Biology, 2003
Microtubule nucleation is the process in which several tubulin molecules interact to form a microtubule seed. Microtubule nucleation occurs spontaneously in purified tubulin solutions, and molecular intermediates between tubulin dimers and microtubules have been identified.
Didier, Job, Odile, Valiron, Berl, Oakley   +2 more
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Spontaneous nucleation of microtubules

Physical Review E, 1995
The spontaneous nucleation of individual microtubules from tubulin dimers is observed directly under conditions of marginal nucleation. The results are compared with measurements of bulk turbidity following a deep quench [W. A. Voter and H. P. Erickson, J. Biol. Chem. 259, 10 430 (1984)].
, Kuchnir Fygenson D, , Flyvbjerg, , Sneppen, , Libchaber, , Leibler   +4 more
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Centrosome-microtubule nucleation

Journal of Cell Science, 1997
ABSTRACT In many cell types the formation of microtubules from tubulin subunits is initiated at defined nucleation sites at the centrosome. Thesse sites contain the conserved γ-tubulin which is in association with additional not very well characterised proteins, identified as components of a γ-tubulin ring complex from Xenopus egg ...
Pereira, G, Schiebel, E
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Regulation of microtubule nucleation mediated by γ-tubulin complexes

Protoplasma, 2017
Pavel Draber, Anastasiya Klebanovych, Vadym Sulimenko   +2 more
exaly   +2 more sources

Microtubule nucleation: The waltz between γ-tubulin ring complex and associated proteins.

Current Opinion in Cell Biology, 2020
Microtubules are essential cytoskeletal elements assembled from αβ-tubulin dimers. In high eukaryotes, microtubule nucleation, the de novo assembly of a microtubule from its minus end, is initiated by the γ-tubulin ring complex (γ-TuRC).
Peng Liu, Martin Würtz, E. Zupa, Stefan Pfeffer, E. Schiebel   +4 more
semanticscholar   +1 more source

Microtubule-associated proteins control the kinetics of microtubule nucleation

Nature Cell Biology, 2015
Microtubules are born and reborn continuously, even during quiescence. These polymers are nucleated from templates, namely γ-tubulin ring complexes (γ-TuRCs) and severed microtubule ends. Using single-molecule biophysics, we show that nucleation from γ-TuRCs, axonemes and seed microtubules requires tubulin concentrations that lie well above the ...
Michal, Wieczorek, Susanne, Bechstedt, Sami, Chaaban, Gary J, Brouhard   +3 more
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