Results 161 to 170 of about 335,784 (179)
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γ-Tubulin complexes and microtubule nucleation
Current Opinion in Structural Biology, 2001Microtubules are dynamic cytoskeletal polymers that assemble from alpha/beta-tubulin and are vital for the establishment of cell polarity, vesicle trafficking and formation of the mitotic/meiotic spindle. gamma-Tubulin, a protein related to alpha/beta-tubulin, is required for initiating the polymerization of microtubules in vivo. gamma-Tubulin has been
M, Moritz, D A, Agard
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Microtubule assembly nucleated by isolated centrosomes
Nature, 1984Microtubules are involved in the morphogenesis of most cells and are the structural basis of the mitotic spindle. We report here that purified centrosomes nucleate the assembly of microtubules with unusual dynamic properties. This may have important implications for the mechanism by which microtubule arrays are organized and stabilized in cells.
T, Mitchison, M, Kirschner
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Microtubule Assembly and Nucleation
1978Publisher Summary This chapter presents recent studies on in vitro assembly, nucleation, and growth of microtubules along with the properties that shed light on their biological role. The biochemical study of structural proteins such as tubulin and actin is a new area.
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The role of nucleation in patterning microtubule networks
Journal of Cell Science, 1998ABSTRACT Control of microtubule nucleation is important for many microtubule dependent processes in cells. Traditionally, research has focused on nucleation of microtubules from centrosomes. However, it is clear that microtubules can nucleate from non-centrosome dependent sites.
A, Hyman, E, Karsenti
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Nucleating microtubules in neurons: Challenges and solutions
Developmental Neurobiology, 2020AbstractThe highly polarized morphology of neurons is crucial for their function and involves formation of two distinct types of cellular extensions, the axonal and dendritic compartments. An important effector required for the morphogenesis and maintenance and thus the identity of axons and dendrites is the microtubule cytoskeleton.
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Reconstitution of centrosome microtubule nucleation in Drosophila
2001Publisher Summary This chapter describes the in vitro complementation assay to determine that the γ-tubulin ring complex (γ-TuRC) is required for Drosophila centrosomes to nucleate microtubules. The assay is based on earlier studies of complementation of inactivated mammalian centrosomes by Xenopus egg extract.
M, Moritz +3 more
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Reconstitution of centrosome microtubule nucleation in Spisula
2001Evidence strongly supports the hypothesis that gamma-TuRCs are sites for the nucleation of microtubules within the centrosome PCM (Zheng et al., 1995; Moritz et al., 1995a,b). Further, these structures appear to be recruited to the centrosome from cytoplasmic pools during centrosome assembly and centrosome maturation (Moritz et al., 1998; Schnackenberg
B J, Schnackenberg, R E, Palazzo
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XMAP215 joins microtubule nucleation team
Nature Cell Biology, 2018De novo assembly of microtubules, nucleation, has remained surprisingly enigmatic, considering that microtubules are polymers of only two proteins, α- and β-tubulin, and that γ-tubulin has a well-established role as nucleator. Now, the tubulin polymerase XMAP215 is shown to be required for efficient nucleation in cooperation with γ-tubulin.
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Acentrosomal microtubule nucleation in higher plants
2002Higher plants have developed a unique pathway to control their cytoskeleton assembly and dynamics. In most other eukaryotes, microtubules are nucleated in vivo at the nucleation and organizing centers and are involved in the establishment of polarity.
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Microtubule nucleation: The waltz between γ-tubulin ring complex and associated proteins
Current Opinion in Cell Biology, 2021Stefan Pfeffer +2 more
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