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Isolation of Microvascular Endothelial Cells

Bio-Protocol, 2018
The vascular endothelium is essential to normal vascular homeostasis. Its dysfunction participates in various cardiovascular disorders. Murine endothelial cell culture is an important tool for cardiovascular disease research. This protocol demonstrates a
Kenneth Cheung, Federica Marelli-Berg
doaj   +4 more sources

Japanese encephalitis virus-associated human microglia induce cell death of human microvascular endothelial cells in receptor-independent infection [PDF]

Frontiers in Cellular and Infection Microbiology
IntroductionThe neurotropic virus Japanese encephalitis virus invades the human central nervous system, inducing neuroinflammation and further disruption of the blood-brain barrier.
Isabelle Fellay, Pauline Blanc, Alexey Larionov, Léa Schlunke, Léa Schlunke, Luis Filgueira, Nils Lannes   +6 more
doaj   +2 more sources

Microvascular endothelial cells of the corpus luteum [PDF]

Reproductive Biology and Endocrinology, 2003
The cyclic nature of the capillary bed in the corpus luteum offers a unique experimental model to examine the life cycle of endothelial cells, involving discrete physiologically regulated steps of angiogenesis, blood vessel maturation and blood vessel ...
Spanel-Borowski Katherina, Rueda Bo R, Davis John S   +2 more
doaj   +3 more sources

Pulmonary endothelial cells from different vascular segments exhibit unique recovery from acidification and Na+/H+ exchanger isoform expression

PLoS ONE, 2022
Sodium-hydrogen exchangers (NHEs) tightly regulate intracellular pH (pHi), proliferation, migration and cell volume. Heterogeneity exists between pulmonary endothelial cells derived from different vascular segments, yet the activity and isoform ...
Dylan Adams, Chung-Sik Choi, Sarah L. Sayner   +2 more
doaj   +2 more sources

Influenza A virus infects pulmonary microvascular endothelial cells leading to microvascular leakage and release of pro-inflammatory cytokines [PDF]

PeerJ, 2021
Objective To investigate the replication of influenza A virus A/Puerto Rico/8/34 (H1N1) in pulmonary microvascular endothelial cells and its effect on endothelial barrier function.
Tiantian Han, Yanni Lai, Yong Jiang, Xiaohong Liu, Danhua Li   +4 more
doaj   +2 more sources

Integrin binding peptides facilitate growth and interconnected vascular-like network formation of rat primary cortical vascular endothelial cells in vitro

Neural Regeneration Research, 2023
Neovascularization and angiogenesis in the brain are important physiological processes for normal brain development and repair/regeneration following insults.
Ram Kuwar, Xuejun Wen, Ning Zhang, Dong Sun   +3 more
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Lactate dehydrogenase a expression is necessary to sustain rapid angiogenesis of pulmonary microvascular endothelium. [PDF]

PLoS ONE, 2013
Angiogenesis is a fundamental property of endothelium, yet not all endothelial cells display equivalent angiogenic responses; pulmonary microvascular endothelial cells undergo rapid angiogenesis when compared to endothelial cells isolated from conduit ...
Glenda Parra-Bonilla, Diego F Alvarez, Mikhail Alexeyev, Audrey Vasauskas, Troy Stevens   +4 more
doaj   +1 more source

Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells

Advanced Healthcare Materials, 2023
ABSTRACTChirality is an intrinsic cellular property that describes cell polarization biases along the left-right axis, apicobasal axis, or front-rear axes. Cell chirality plays a significant role in the arrangement of organs in the body as well as the orientation of organelles, cytoskeletons, and cells.
Samantha G. Zambuto, Ishita Jain, Hannah S. Theriault, Gregory H. Underhill, Brendan A.C. Harley   +4 more
openaire   +3 more sources

Retroviral transformation of cerebral microvascular endothelial cells: macrophage-like and microvascular endothelial cell properties [PDF]

Blood, 1991
We report that L-cell-conditioned medium (LCM) transforms porcine cerebral microvascular (PCMV) endothelial cells into cells with macrophage-like properties. LCM is known to contain both cytokine(s) and the L-cell virus, a murine retrovirus found in the L929 cell and LCM. Our evidence suggests that both LCM cytokine(s) and the L-cell virus are involved
D H, Robinson, M K, Warren, L T, Liang, J J, Oprandy, T B, Nielsen, Y H, Kang   +5 more
openaire   +3 more sources

Soluble pathogenic tau enters brain vascular endothelial cells and drives cellular senescence and brain microvascular dysfunction in a mouse model of tauopathy

Nature Communications, 2023
Vascular mechanisms of Alzheimer’s disease (AD) may constitute a therapeutically addressable biological pathway underlying dementia. We previously demonstrated that soluble pathogenic forms of tau (tau oligomers) accumulate in brain microvasculature of ...
Stacy A. Hussong, Andy Q. Banh, Candice E. Van Skike, Angela O. Dorigatti, Stephen F. Hernandez, Matthew J. Hart, Beatriz Ferran, Haneen Makhlouf, Maria Gaczynska, Pawel A. Osmulski, Salome A. McAllen, Kelly T. Dineley, Zoltan Ungvari, Viviana I. Perez, Rakez Kayed, Veronica Galvan   +15 more
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