Results 61 to 70 of about 1,401,422 (284)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Dwelling : Heidegger, Archaeology, Mortality
, 2017 Dwelling: Heidegger, Archaeology, Mortality negotiates the discourses of phenomenology, archaeology and palaeoanthropology in order to extend the ‘dwelling perspective’, an approach in the social sciences particularly associated with Tim Ingold and a ...
Tonner, Philip
core +2 more sources
Peak particle velocity data acquisition for monitoring blast induced earthquakes in quarry sites
Data in Brief, 2018 The peak particle velocity datasets recorded during quarry blasts in the neighborhood villages and towns in Ibadan and Abeokuta were processed and analyzed in order to recommend a safe blast design for each of the quarries.
O.S. Hammed +6 more
doaj +1 more source
Identification of switched linear state space models without minimum dwell time [PDF]
IFAC Proceedings Volumes, 2009 We consider the problem of identifying switched linear state space models from a finite set of input-output data. This is a challenging problem, which requires inferring both the discrete state and the parameter matrices associated with each discrete state.
L. Bako +3 more
openaire +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Response of crossbred cattle in terms of respiration rate and rectal temperature to maximum and minimum THI period in Kerala [PDF]
Journal of Veterinary and Animal Sciences, 2020 The present study assesses the relationship of meteorological variables with physiological stress parameters. Respiration rate was continuously measured (RR) using Physiological monitor with improvised facemask.
N.V. Jisha +6 more
doaj
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
AgricultureSoil-dwelling predatory mites (Acari: Mesostigmata) are key components of decomposer-based soil food webs and contribute to the regulation of soil microarthropods, including agricultural pests.
Sergeja Adamič Zamljen +4 more
doaj +1 more source
PLoS ONE, 2013 Aedes aegypti and Ae. albopictus are the vectors of dengue, the most important arboviral disease of humans. To date, Aedes ecology studies have assumed that the vectors are truly absent from sites where they are not detected; since no perfect detection ...
Samael D Padilla-Torres +4 more
doaj +1 more source