Results 21 to 30 of about 472,091 (296)
A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
BMC Biotechnology, 2010 Background The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences.
Nørholm Morten HH
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Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice
Czech Journal of Genetics and Plant Breeding, 2022 Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism,
Yu Li +6 more
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Metabolic Engineering Communications, 2016 Molecular Cloning Designer Simulator (MCDS) is a powerful new all-in-one cloning and genetic engineering design, simulation and management software platform developed for complex synthetic biology and metabolic engineering projects.
Zhenyu Shi, Claudia E. Vickers
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Stem Cell Research & Therapy, 2019 Background Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models.
Junjun Xu +13 more
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Plants, 2022 Coding sequences of the CLC family genes SaCLCd, SaCLCf, and SaCLCg, the putative orthologs of Arabidopsis thaliana AtCLCd, AtCLCf, and AtCLCg genes, were cloned from the euhalophyte Suaeda altissima (L.) Pall.
Olga I. Nedelyaeva +3 more
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Arguments for Human Therapeutic Cloning
Biomolecules & Biomedicine, 2008 For over 30 years, many Western governments have regulated scientific research involving human subjects. According to Knoppers implementation of regulation followed a long and checkered history of research abuse.
Krešimir Pavelić
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Molecular cloning of cat interleukin-12 [PDF]
Immunogenetics, 1997 The recently discovered interleukin-12 (IL-12) is a heterodimeric 70000 Mr cytokine composed of a disulphide-bonded 35000 Mr (p35) subunit and a 40000 Mr (p40) subunit. Produced mainly by activated monocytes/macrophages and B cells, it enhances NK/LAK cell cytolytic activity and stimulates cytotoxic T-cell responses and IFN-γ production.
Horzinek, M.C. +3 more
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Recombination-assisted megaprimer (RAM) cloning
MethodsX, 2014 No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive. Restriction-free cloning is among the simplest, most rapid, and cost-effective methods, but does not always provide ...
Jacques Mathieu +2 more
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Molecular cloning of duck CD40 and its immune function research
Poultry Science, 2021 : Cosignal molecules are cell surface molecules that transduce signals to other cells to modulate immune response positively (costimulate) or negatively (cosuppress).
Rujuan Zhang +19 more
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An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
FEBS Letters, EarlyView.This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
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