Results 121 to 130 of about 230,773 (293)

High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function

Molecular Neurodegeneration, 2010
Background Cultured spinal motor neurons are a valuable tool to study basic mechanisms of development, axon growth and pathfinding, and, importantly, to analyze the pathomechanisms underlying motor neuron diseases.
Bassell Gary J, Fallini Claudia, Rossoll Wilfried   +2 more
doaj   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

The Development of ELISA and SPR-Based Immunoassays for the Detection of Heat Shock Proteins. [PDF]

, 2009
Heat shock proteins (Hsps) are highly conserved molecules in all eukaryotes and prokaryotes. They are named on the basis of their molecular weight and their synthesis is up-regulated by stress conditions such as inflammation, oxidative stress and ...
Gong, Bei
core  

Molecular Mechanisms and Potential Biomarkers of Neuropsychiatric Disorders in Parkinson’s Disease

Journal of Integrative Neuroscience
Parkinson’s disease (PD) is the second most common degenerative disease of the central nervous system, characterized by both motor and non-motor disorders. Non-motor disorders include dementia, depression, and anxiety.
Wojciech Owecki, Anna Futyma, Olga Kokorniak, Aleksandra Szczech, Bartosz Słowikowski, Joanna Poszwa, Oliwia Szymanowicz, Ulyana Goutor, Paweł P. Jagodziński, Wojciech Kozubski, Jolanta Dorszewska   +10 more
doaj   +1 more source

Assembly of Protein Molecular Motors for Nano-Bio-Machines

Seibutsu Butsuri, 2005
Protein nanomachines have many attractive features. Whether we can take advantage of those depends on future technical advancements to reconstitute their functions in vitro in a useful manner. Breakthroughs towards this direction have been made in the field of protein molecular motors.
HIRATSUKA, Yuichi, UYEDA, Taro Q. P.
openaire   +2 more sources

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Dissecting the Molecular Mechanisms of Motor Neurone Disease in Yeast and Worms [PDF]

Superoxide dismutase 1 (SOD1) is an enzyme responsible for intracellular breakdown of toxic reactive oxygen species in prokaryotes and eukaryotes. Mutations in SOD1 are linked to the motor neurone disease Amyotrophic lateral sclerosis (ALS), with around ...
Peswani, Amber Rose
core  

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

A molecular analysis of the microtubule associated protein MAP65-1 [PDF]

, 2006
Microtubules (MTs) play important roles in various cellular processes including cell division, organelle movement and the determination of cellular morphology. The dynamics and organization of microtubules are regulated by microtubule associated proteins
Hsin Yu, Chang
core  

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source