Human dynein-dynactin is a fast processive motor in living cells. [PDF]
ElifeMinus-end directed transport along microtubules in eukaryotes is primarily mediated by cytoplasmic dynein and its cofactor dynactin. Significant advances have been made in recent years characterizing human dynein–dynactin structure and function using in ...
Verma V, Wadsworth P, Maresca TJ.
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Cryo-ET and MD simulations reveal that dynein-2 is tuned for binding to the A-tubule of the ciliary doublet. [PDF]
EMBO JEukaryotic cilia and flagella are thin structures present on the surface of cells, playing vital roles in signaling and cellular motion. Cilia assembly depends on intraflagellar transport (IFT) along doublet microtubules (doublets).
He HK +5 more
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Cryo-EM captures early intermediate steps in dynein activation by LIS1. [PDF]
Nat CommunCytoplasmic dynein-1 (dynein) is an essential molecular motor in eukaryotic cells. Dynein primarily exists in an autoinhibited Phi state and requires conformational changes to assemble with its cofactors and form active transport complexes.
Nguyen KHV +4 more
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eLife, 2022 Mutations in the microtubule (MT)-binding protein doublecortin (DCX) or in the MT-based molecular motor dynein result in lissencephaly. However, a functional link between DCX and dynein has not been defined.
Xiaoqin Fu +7 more
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The KASH5 protein involved in meiotic chromosomal movements is a novel dynein activating adaptor
eLife, 2022 Dynein harnesses ATP hydrolysis to move cargo on microtubules in multiple biological contexts. Dynein meets a unique challenge in meiosis by moving chromosomes tethered to the nuclear envelope to facilitate homolog pairing essential for gametogenesis ...
Ritvija Agrawal +7 more
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Structures of human dynein in complex with the lissencephaly 1 protein, LIS1
eLife, 2023 The lissencephaly 1 protein, LIS1, is mutated in type-1 lissencephaly and is a key regulator of cytoplasmic dynein-1. At a molecular level, current models propose that LIS1 activates dynein by relieving its autoinhibited form.
Janice M Reimer +3 more
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FBB18 participates in preassembly of almost all axonemal dyneins independent of R2TP complex.
PLoS Genetics, 2022 Assembly of dynein arms requires cytoplasmic processes which are mediated by dynein preassembly factors (DNAAFs). CFAP298, which is conserved in organisms with motile cilia, is required for assembly of dynein arms but with obscure mechanisms.
Limei Wang +3 more
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Cargo-Mediated Activation of Cytoplasmic Dynein in vivo
Frontiers in Cell and Developmental Biology, 2020 Cytoplasmic dynein-1 is a minus-end-directed microtubule motor that transports a variety of cargoes including early endosomes, late endosomes and other organelles.
Xin Xiang, Rongde Qiu
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Dynamic regulation of dynein localization revealed by small molecule inhibitors of ubiquitination enzymes [PDF]
Open Biology, 2018 Cytoplasmic dynein is a minus-end-directed microtubule-based motor that acts at diverse subcellular sites. During mitosis, dynein localizes simultaneously to the mitotic spindle, spindle poles, kinetochores and the cell cortex.
Julie K. Monda, Iain M. Cheeseman
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Nde1 promotes Lis1-mediated activation of dynein
Nature Communications, 2023 Cytoplasmic dynein drives the motility and force generation functions towards the microtubule minus end. The assembly of dynein with dynactin and a cargo adaptor in an active transport complex is facilitated by Lis1 and Nde1/Ndel1.
Yuanchang Zhao, Sena Oten, Ahmet Yildiz
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