Results 31 to 40 of about 39,736 (248)

Structural basis for cytoplasmic dynein-1 regulation by Lis1

eLife, 2022
The lissencephaly 1 gene, LIS1, is mutated in patients with the neurodevelopmental disease lissencephaly. The Lis1 protein is conserved from fungi to mammals and is a key regulator of cytoplasmic dynein-1, the major minus-end-directed microtubule motor ...
John P Gillies, Janice M Reimer, Eva P Karasmanis, Indrajit Lahiri, Zaw Min Htet, Andres E Leschziner, Samara L Reck-Peterson   +6 more
doaj   +1 more source

Dynein light intermediate chains as pivotal determinants of dynein multifunctionality [PDF]

Journal of Cell Science, 2021
ABSTRACT In animal cells, a single cytoplasmic dynein motor mediates microtubule minus-end-directed transport, counterbalancing dozens of plus-end-directed kinesins. The remarkable ability of dynein to interact with a diverse cargo spectrum stems from its tightly regulated recruitment of cargo-specific adaptor proteins, which engage the ...
Amrita Kumari, Chandan Kumar, Neeraj Wasnik, Sivaram V. S. Mylavarapu   +3 more
openaire   +4 more sources

Dynein and intraflagellar transport [PDF]

Experimental Cell Research, 2015
Publisher Summary This chapter provides a brief background on intraflagellar transport (IFT) and reviews the studies culminating in the identification of the dynein motor that powers retrograde IFT. IFT is the active movement of multi-subunit particles along axonemal doublet microtubules in the space between the outer-doublet microtubules and the ...
Hou, Yuqing, Witman, George B.
openaire   +3 more sources

Nde1 and Ndel1: Outstanding Mysteries in Dynein-Mediated Transport

Frontiers in Cell and Developmental Biology, 2022
Cytoplasmic dynein-1 (dynein) is the primary microtubule minus-end directed molecular motor in most eukaryotes. As such, dynein has a broad array of functions that range from driving retrograde-directed cargo trafficking to forming and focusing the ...
Sharon R. Garrott, Sharon R. Garrott, John P. Gillies, Morgan E. DeSantis, Morgan E. DeSantis   +4 more
doaj   +1 more source

Force measurements with optical tweezers inside living cells [PDF]

, 2014
The force exerted by optical tweezers can be measured by tracking the momentum changes of the trapping beam, a method which is more general and powerful than traditional calibration techniques as it is based on first principles, but which has not been ...
Farre, A., Martin-Badosa, E., Josep Mas, Jordi Sancho-Parramon, Arnau Farré, Sancho-Parramon, J., Montes-Usategui, M., Mario Montes-Usategui, Mas, J., Estela Martín-Badosa   +9 more
core   +1 more source

Cytoplasmic dynein nomenclature [PDF]

The Journal of Cell Biology, 2005
A variety of names has been used in the literature for the subunits of cytoplasmic dynein complexes. Thus, there is a strong need for a more definitive consensus statement on nomenclature. This is especially important for mammalian cytoplasmic dyneins, many subunits of which are encoded by multiple genes.
Pfister, K. Kevin, Fisher, Elizabeth M. C., Gibbons, Ian R., Hays, Thomas S., Holzbaur, Erika L. F., McIntosh, J. Richard, Porter, Mary E., Schroer, Trina A., Vaughan, Kevin T., Witman, George B., King, Stephen M., Vallee, Richard B.   +11 more
openaire   +3 more sources

Lissencephaly-1 is a context-dependent regulator of the human dynein complex

eLife, 2017
The cytoplasmic dynein-1 (dynein) motor plays a central role in microtubule organisation and cargo transport. These functions are spatially regulated by association of dynein and its accessory complex dynactin with dynamic microtubule plus ends. Here, we
Janina Baumbach, Andal Murthy, Mark A McClintock, Carly I Dix, Ruta Zalyte, Ha Thi Hoang, Simon L Bullock   +6 more
doaj   +1 more source

Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1

eLife, 2018
Cortical dynein generates pulling forces via microtubule (MT) end capture-shrinkage and lateral MT sliding mechanisms. In Saccharomyces cerevisiae, the dynein attachment molecule Num1 interacts with endoplasmic reticulum (ER) and mitochondria to ...
Safia Omer, Samuel R Greenberg, Wei-Lih Lee   +2 more
doaj   +1 more source

Regulation of in vivo dynein force production by CDK5 and 14-3-3ε and KIAA0528

Nature Communications, 2019
Dynein plays roles in vesicular, organelle, chromosomal and nuclear transport but so far it is unclear how dynein activity in cells is regulated. Here authors study several dynein cofactors and their role in force adaptation of dynein during lipid ...
Dail E. Chapman, Babu J. N. Reddy, Bunchhin Huy, Matthew J. Bovyn, Stephen John S. Cruz, Zahraa M. Al-Shammari, Han Han, Wenqi Wang, Deanna S. Smith, Steven P. Gross   +9 more
doaj   +1 more source

Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function

eLife, 2018
The dynein-2 microtubule motor is the retrograde motor for intraflagellar transport. Mutations in dynein-2 components cause skeletal ciliopathies, notably Jeune syndrome. Dynein-2 contains a heterodimer of two non-identical intermediate chains, WDR34 and
Laura Vuolo, Nicola L Stevenson, Kate J Heesom, David J Stephens   +3 more
doaj   +1 more source