Determining cellular CTCF and cohesin abundances to constrain 3D genome models. [PDF]
, 2019 Achieving a quantitative and predictive understanding of 3D genome architecture remains a major challenge, as it requires quantitative measurements of the key proteins involved.
Cattoglio, Claudia +11 more
core +2 more sources
Distribution of the Escherichia coli structural maintenance of chromosomes (SMC)‐like protein MukB in the cell [PDF]
Molecular Microbiology, 2001 Fluorescent polyclonal antibodies specific for MukB have been used to study its localization in Escherichia coli. In wild‐type cells, the MukB protein appeared as a limited number of oblong shapes embracing the nucleoid. MukB remained associated with the nucleoid in the absence of DNA replication.
den Blaauwen, T. +3 more
openaire +4 more sources
Prospect for conservation agriculture in Northern and Eastern European countries, lessons of Kassa [PDF]
, 2006 SYNOPSIS. In Europe, Conservation agriculture (CA) is less adopted than in the other world adopting regions and, reduced tillage is more used than no-tillage. Use of cover crop is not common.
Barz, P. +9 more
core
Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli [PDF]
Proceedings of the National Academy of Sciences, 2010 Proper geometric and topological organization of DNA is essential for all chromosomal processes. Two classes of proteins play major roles in organizing chromosomes: condensin complexes and type II topoisomerases. In Escherichia coli , MukB, a structural maintenance of chromosome-like component of the bacterial ...
Ryo, Hayama, Kenneth J, Marians
openaire +2 more sources
Cohesin biology meets the loop extrusion model [PDF]
, 2017 Extensive research has revealed that cohesin acts as a topological device, trapping chromosomal DNA within a large tripartite ring. In so doing, cohesin contributes to the formation of compact and organized genomes.
Barrington, C, Finn, R, Hadjur, S
core +1 more source
The Crystal Structure of the Hinge Domain of the Escherichia coli Structural Maintenance of Chromosomes Protein MukB [PDF]
Journal of Molecular Biology, 2010 MukB, a divergent structural maintenance of chromosomes (SMC) protein, is important for chromosomal segregation and condensation in gamma-proteobacteria. MukB and canonical SMC proteins share a characteristic five-domain structure. Globular N- and C-terminal domains interact to form an ATP-binding cassette-like ATPase or "head" domain, which is ...
Yinyin, Li +3 more
openaire +2 more sources
Global transcriptional response of O157:H7 to growth transitions in glucose minimal medium [PDF]
, 2007 Background: Global patterns of gene expression of Escherichia coli K-12 during growth transitions have been deeply investigated, however, comparable studies of E.
Teresa M Bergholz +5 more
core +2 more sources
Different localization of SeqA‐bound nascent DNA clusters and MukF–MukE–MukB complex in Escherichia coli cells [PDF]
Molecular Microbiology, 2001 MukF, MukE and MukB proteins form a complex that may participate in the organization of folded sister chromosomes in Escherichia coli. We have found that a MukB–GFPuv4 fusion protein is observed as discrete fluorescent foci, which are localized within cellular spaces occupied by nucleoids, but not at the constriction site of cell division in living ...
K, Ohsumi, M, Yamazoe, S, Hiraga
openaire +2 more sources
In vitro topological loading of bacterial condensin MukB on DNA, preferentially single-stranded DNA rather than double-stranded DNA [PDF]
Scientific Reports, 2016 AbstractCondensin is the major driving force in the segregation of daughter chromosomes in prokaryotes. Core subunits of condensin belong to the SMC protein family, whose members are characterized by a unique ATPase activity and dimers with a V-shaped structure.
Hironori Niki, Koichi Yano
openaire +2 more sources
Comparative analyses of proteins from Haemophilus influenzae biofilm and planktonic populations using metabolic labeling and mass spectrometry [PDF]
, 2014 BACKGROUND: Non-typeable H. influenzae (NTHi) is a nasopharyngeal commensal that can become an opportunistic pathogen causing infections such as otitis media, pneumonia, and bronchitis. NTHi is known to form biofilms.
Apicella, Michael A +6 more
core +2 more sources