RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli [PDF]
Journal of Bacteriology, 1996 We have isolated suppressor mutants that suppress temperature-sensitive colony formation and anucleate cell production of a mukB mutation. A linkage group (smbB) of the suppressor mutations is located in the rne/ams/hmp gene encoding the processing endoribonuclease RNase E. All of the rne (smbB) mutants code for truncated RNase E polypeptides lacking a
M, Kido +5 more
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Two mutant alleles ofmukB, a gene essential for chromosome partition inEscherichia coli [PDF]
FEMS Microbiology Letters, 1994 The MukB protein is essential for chromosome partitioning in Escherichia coli and consists of 1484 amino acid residues (170 kDa). We have determined the base changes at the mutated sites of the mukB106 mutant and a newly isolated mutant, mukB33. These mutant mukB genes were each found to carry a single base-pair transition which leads to an amino acid ...
K, Yamanaka +5 more
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mBio, 2014 The Escherichia coli structural maintenance of chromosome (SMC) complex, MukBEF, and topoisomerase IV (TopoIV) interact in vitro through a direct contact between the MukB dimerization hinge and the C-terminal domain of ParC, the catalytic subunit of ...
Emilien Nicolas +5 more
doaj +1 more source
High-resolution definition of the Vibrio cholerae essential gene set with hidden Markov model–based analyses of transposon-insertion sequencing data [PDF]
, 2014 The coupling of high-density transposon mutagenesis to high-throughput DNA sequencing (transposon-insertion sequencing) enables simultaneous and genome-wide assessment of the contributions of individual loci to bacterial growth and survival.
Chao, Michael C. +6 more
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Biochemical characterization of the Structural Maintenance of Chromosomes (SMC) complex from Bacillus subtilis [PDF]
, 2004 Structural Maintenance of Chromosomes (SMC) proteins play a key role in the chromosome dynamics throughout the cell cycle in almost all species from bacteria to eukaryotes.
Graumann, Peter (PD Dr.), Volkov, Arsen
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Dissecting DNA Compaction by the Bacterial Condensin MukB
, 2019 Condensins in bacteria are one of the most important factors involved in the organization of long threads of DNA into compact chromosomes. The organization of DNA by condensins is vital to many DNA transactions including DNA repair and chromosome segregation.
Rupesh, Kumar +2 more
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Role of the mukB gene in chromosome and plasmid partition in Escherichia coli [PDF]
Molecular Microbiology, 2000 The intracellular locations of oriC and oriR1, the replication origins of the chromosome and plasmid R1, respectively, were visualized by fluorescence in situ hybridization (FISH) in exponentially growing populations of Escherichia coli. The locations of oriC and oriR1 (from a Par+ R1 plasmid) were unique and different in the wild‐type host.
T, Weitao, S, Dasgupta, K, Nordström
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HEAT repeats associated with condensins, cohesins, and other complexes involved in chromosome-related functions [PDF]
, 2000 HEAT repeats correspond to tandemly arranged curlicue-like structures that appear to serve as flexible scaffolding on which other components can assemble.
Neuwald, A. F., Hirano, T.
core +1 more source
Are SMC complexes loop extruding factors? Linking theory with fact [PDF]
, 2019 The extreme length of chromosomal DNA requires organizing mechanisms to both promote functional genetic interactions and ensure faithful chromosome segregation when cells divide.
Akai +102 more
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The constancy of gene conservation across divergent bacterial orders [PDF]
, 2009 Background. Orthologous genes are frequently presumed to perform similar functions. However, outside of model organisms, this is rarely tested. One means of inferring changes in function is if there are changes in the level of gene conservation and ...
Olin K Silander, Martin Ackermann
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