Patterns of gene set activity during MukB induction.
, 2013 Gene set scores computed using gene expression responses after 2.5(A2), 5 h (A3) and 7.5 h (A4) post MukB induction, were sorted based on similarity of profiles into four clusters.
Valentin V. Rybenkov (501513) +4 more
core +1 more source
Microorganisms, 2019 Prokaryotes have an essential gene—gyrase—that catalyzes negative supercoiling of plasmid and chromosomal DNA. Negative supercoils influence DNA replication, transcription, homologous recombination, site-specific recombination, genetic ...
Nikolay S. Rovinskiy +3 more
doaj +1 more source
Interactions of the ACP mutants with SpoT and MukB. A.
, 2012 Co-purification on calmodulin beads was performed as described in Experimental procedures on extracts prepared from cultures of W3110SpoT-SG transformed with the indicated pT18-ACP mutant plasmids. Purified samples were analyzed by SDS-PAGE (10% for MukB
Emmanuelle Bouveret (168415) +2 more
core +1 more source
Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex in Live Escherichia coli Bacteria [PDF]
, 2022 MukBEF, a structural maintenance of chromosome (SMC) complex, is an important molecular machine for chromosome organization and segregation in Escherichia coli. Fluorescently tagged MukBEF forms distinct spots (or "foci") composed of molecular assemblies
Badrinarayanan, Anjana, Leake, Mark C
core +1 more source
Distribution of the Escherichia coli structural maintenance of chromosomes (SMC)‐like protein MukB in the cell [PDF]
Molecular Microbiology, 2001 Fluorescent polyclonal antibodies specific for MukB have been used to study its localization in Escherichia coli. In wild‐type cells, the MukB protein appeared as a limited number of oblong shapes embracing the nucleoid. MukB remained associated with the nucleoid in the absence of DNA replication.
den Blaauwen, T. +3 more
openaire +4 more sources
Molecular Microbiology, Volume 122, Issue 1, Page 81-112, July 2024.In this review article, the diverse mechanisms by which bacterial chromatin proteins influence and are influenced by transcription and DNA supercoiling are discussed. Abstract DNA in bacterial chromosomes is organized into higher‐order structures by DNA‐binding proteins called nucleoid‐associated proteins (NAPs) or bacterial chromatin proteins (BCPs ...
Christine M. Hustmyer, Robert Landick
wiley +1 more source
RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli [PDF]
Journal of Bacteriology, 1996 We have isolated suppressor mutants that suppress temperature-sensitive colony formation and anucleate cell production of a mukB mutation. A linkage group (smbB) of the suppressor mutations is located in the rne/ams/hmp gene encoding the processing endoribonuclease RNase E. All of the rne (smbB) mutants code for truncated RNase E polypeptides lacking a
M, Kido +5 more
openaire +2 more sources
MukB colocalizes with the oriC region and is required for organization of the two Escherichia coli chromosome arms into separate cell halves. [PDF]
, 2007 The circular Escherichia coli chromosome is organized by bidirectional replication into two equal left and right arms (replichores). Each arm occupies a separate cell half, with the origin of replication (oriC) at mid-cell. E.
Pinskaya, M +14 more
core +1 more source
List of gene sets active in at least one of the 3 mukB++ conditions.
, 2013 List of gene sets active in at least one of the 3 mukB++ conditions.
Valentin V. Rybenkov (501513) +4 more
core +1 more source
The MatP/matS Site-Specific System Organizes the Terminus Region of the E. coli Chromosome into a Macrodomain [PDF]
, 2008 The organization of the Escherichia coli chromosome into insulated macrodomains influences the segregation of sister chromatids and the mobility of chromosomal DNA.
El Karoui, Meriem +7 more
core +1 more source