Results 31 to 40 of about 829 (144)

Identification and characterization of the smbA gene, a suppressor of the mukB null mutant of Escherichia coli [PDF]

Journal of Bacteriology, 1992
The mukB gene encodes a protein involved in chromosome partitioning in Escherichia coli. To study the function of this protein, we isolated from the temperature-sensitive mukB null mutant and characterized 56 suppressor mutants which could grow at 42 degrees C. Ten of the mutants also showed cold-sensitive growth at 22 degrees C.
K, Yamanaka, T, Ogura, H, Niki, S, Hiraga   +3 more
openaire   +2 more sources

Escherichia coli CrfC Protein, a Nucleoid Partition Factor, Localizes to Nucleoid Poles via the Activities of Specific Nucleoid-Associated Proteins

Frontiers in Microbiology, 2019
The Escherichia coli CrfC protein is an important regulator of nucleoid positioning and equipartition. Previously we revealed that CrfC homo-oligomers bind the clamp, a DNA-binding subunit of the DNA polymerase III holoenzyme, promoting colocalization of
Saki Taniguchi, Kazutoshi Kasho, Shogo Ozaki, Tsutomu Katayama   +3 more
doaj   +1 more source

Role of the mukB gene in chromosome and plasmid partition in Escherichia coli [PDF]

Molecular Microbiology, 2000
The intracellular locations of oriC and oriR1, the replication origins of the chromosome and plasmid R1, respectively, were visualized by fluorescence in situ hybridization (FISH) in exponentially growing populations of Escherichia coli. The locations of oriC and oriR1 (from a Par+ R1 plasmid) were unique and different in the wild‐type host.
T, Weitao, S, Dasgupta, K, Nordström
openaire   +2 more sources

The Localization and Action of Topoisomerase IV in Escherichia coli Chromosome Segregation Is Coordinated by the SMC Complex, MukBEF

Cell Reports, 2015
The type II topoisomerase TopoIV, which has an essential role in Escherichia coli chromosome decatenation, interacts with MukBEF, an SMC (structural maintenance of chromosomes) complex that acts in chromosome segregation.
Pawel Zawadzki, Mathew Stracy, Katarzyna Ginda, Katarzyna Zawadzka, Christian Lesterlin, Achillefs N. Kapanidis, David J. Sherratt   +6 more
doaj   +1 more source

The SMC Complex MukBEF Recruits Topoisomerase IV to the Origin of Replication Region in Live Escherichia coli

mBio, 2014
The Escherichia coli structural maintenance of chromosome (SMC) complex, MukBEF, and topoisomerase IV (TopoIV) interact in vitro through a direct contact between the MukB dimerization hinge and the C-terminal domain of ParC, the catalytic subunit of ...
Emilien Nicolas, Amy L. Upton, Stephan Uphoff, Olivia Henry, Anjana Badrinarayanan, David Sherratt   +5 more
doaj   +1 more source

Two mutant alleles ofmukB, a gene essential for chromosome partition inEscherichia coli [PDF]

FEMS Microbiology Letters, 1994
The MukB protein is essential for chromosome partitioning in Escherichia coli and consists of 1484 amino acid residues (170 kDa). We have determined the base changes at the mutated sites of the mukB106 mutant and a newly isolated mutant, mukB33. These mutant mukB genes were each found to carry a single base-pair transition which leads to an amino acid ...
K, Yamanaka, T, Mitani, J, Feng, T, Ogura, H, Niki, S, Hiraga   +5 more
openaire   +3 more sources

ATP-Induced Shrinkage of DNA with MukB Protein and the MukBEF Complex of Escherichia coli [PDF]

Journal of Bacteriology, 2008
ABSTRACT Fluorescence microscopic observation of individual T4 DNA molecules revealed that the MukBEF complex (bacterial condensin) and its subunit, the MukB (a member of the SMC [structural maintenance of chromosomes] superfamily) homodimer, of Escherichia coli markedly shrunk large DNA molecules in the ...
Ning, Chen, Anatoly A, Zinchenko, Yuko, Yoshikawa, Sumiko, Araki, Shun, Adachi, Mitsuyoshi, Yamazoe, Sota, Hiraga, Kenichi, Yoshikawa   +7 more
openaire   +2 more sources

Molecular basis for SMC rod formation and its dissolution upon DNA binding. [PDF]

, 2015
SMC condensin complexes are central modulators of chromosome superstructure in all branches of life. Their SMC subunits form a long intramolecular coiled coil, which connects a constitutive "hinge" dimerization domain with an ATP-regulated "head ...
Soh, Young-Min, Stephan Gruber, Kim, Cheolhee, Kim, Y.-G., Kim, YG, Oh, B., Bürmann, F., Soh, Y., Shin, H.-C., Oh, Byung-Ha, Shin, HC, Lee, H., Durand-Diebold, M., Jin, KS, S. Gruber, Lee, Hansol, Kim, Ho Min, Shin, Ho-Chul, Kim, HM, Buermann, Frank, Toseland, C., Kim, H.M., Oda, T, Soh, YM, Durand-Diebold, Marie-Laure, -Diebold, ML, Kim, Y., Gruber, S., Jin, Kyeong Sik, Bürmann, Frank, Oh, BH, Durand-Diebold, M.-L., Kim, Yeon-Gil, Kong, Min-Seok, Kim, C, Oda, Takashi, C. Kim, Lee, Nam Ki, M-L Durand-Diebold, Lee, N.K., Kim, H., Kim, Soo Jin, Sato, M., Gruber, S, Toseland, Christopher P., Oda, T., Lee, NK, M-S Kong, Diebold-Durand, Marie-Laure, Lee, N., Y.-G. Kim, Kim, C., Toseland, C.P., Soh, Young Min, Kim, Ho-Min, Gruber, Stephan, Soh, Y.-M., Oh, B.-H., Sato, Mamoru, Buermann, F., Kim, S., Jin, K.S., Shin, H., Kim, S.J., Kong, M.-S., Kong, M., S. J. Kim, Burmann, F, Jin, K., C. P. Toseland   +69 more
core   +1 more source

Dynamic architecture of the Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBEF. [PDF]

Nucleic Acids Res, 2019
Ubiquitous Structural Maintenance of Chromosomes (SMC) complexes use a proteinaceous ring-shaped architecture to organize and individualize chromosomes, thereby facilitating chromosome segregation.
Rajasekar KV, Baker R, Fisher GLM, Bolla JR, Mäkelä J, Tang M, Zawadzka K, Koczy O, Wagner F, Robinson CV, Arciszewska LK, Sherratt DJ.   +11 more
europepmc   +2 more sources

The Crystal Structure of the Hinge Domain of the Escherichia coli Structural Maintenance of Chromosomes Protein MukB [PDF]

Journal of Molecular Biology, 2010
MukB, a divergent structural maintenance of chromosomes (SMC) protein, is important for chromosomal segregation and condensation in gamma-proteobacteria. MukB and canonical SMC proteins share a characteristic five-domain structure. Globular N- and C-terminal domains interact to form an ATP-binding cassette-like ATPase or "head" domain, which is ...
Yinyin, Li, Allyn J, Schoeffler, James M, Berger, Martha G, Oakley   +3 more
openaire   +2 more sources