Results 11 to 20 of about 1,534 (157)

The MukB-topoisomerase IV interaction mutually suppresses their catalytic activities [PDF]

Nucleic Acids Research, 2022
Abstract The bacterial condensin MukB and the cellular chromosomal decatenase, topoisomerase IV interact and this interaction is required for proper condensation and topological ordering of the chromosome. Here, we show that Topo IV stimulates MukB DNA condensation by stabilizing loops in DNA: MukB alone can condense nicked plasmid DNA ...
Rupesh Kumar, Kenneth J Marians
exaly   +5 more sources

MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin

eLife, 2018
The Escherichia coli SMC complex, MukBEF, acts in chromosome segregation. MukBEF shares the distinctive architecture of other SMC complexes, with one prominent difference; unlike other kleisins, MukF forms dimers through its N-terminal domain.
Katarzyna Zawadzka, Pawel Zawadzki, Rachel Baker, Karthik V Rajasekar, Florence Wagner, David J Sherratt, Lidia K Arciszewska   +6 more
doaj   +5 more sources

Corrigendum: Dynamic nature of SecA and Its associated proteins in Escherichia coli [PDF]

Frontiers in Microbiology, 2020
Shun Adachi, Yasuhiro Murakawa, Sota Hiraga   +2 more
doaj   +2 more sources

Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens SMC5/6 complex

The Plant Journal, Volume 115, Issue 4, Page 1084-1099, August 2023., 2023
Significance Statement The understanding of the role of the structural maintenance of chromosomes (SMC) 5/6 complexes in plants, particularly of the NSE5 and NSE6 subunits, is poor. We identified a previously unrecognized NSE6 motif mediating interaction with NSE5 and mapped the Physcomitrium patens PpNSE6‐binding site at the PpSMC5 arm. These findings
Edit Lelkes, Jitka Jemelková, Marcela Holá, Barbora Štefanovie, Peter Kolesár, Radka Vágnerová, Eva Dvořák Tomaštíková, Ales Pecinka, Karel J. Angelis, Jan J. Paleček   +9 more
wiley   +1 more source

Cancer‐associated mutations in the condensin II subunit CAPH2 cause genomic instability through telomere dysfunction and anaphase chromosome bridges

Journal of Cellular Physiology, Volume 236, Issue 5, Page 3579-3598, May 2021., 2021
The condensin II complex mutations R551P, R551S, and S556F cause genomic instability by causing DNA damage, anaphase defects, micronuclei, and chromosomal instability. DNA damage and anaphase defects are caused primarily by ataxia telangiectasia and Rad3‐related‐dependent telomere dysfunction.
Emily Weyburne, Giovanni Bosco
wiley   +1 more source

Transient growth arrest in Escherichia coli induced by chromosome condensation. [PDF]

PLoS ONE, 2013
MukB is a bacterial SMC (structural maintenance of chromosome) protein that regulates the global folding of the Escherichia coli chromosome by bringing distant DNA segments together.
Andrea L Edwards, Dipen P Sangurdekar, Kyeong S Jeong, Arkady B Khodursky, Valentin V Rybenkov   +4 more
doaj   +1 more source

MukB-mediated Catenation of DNA Is ATP and MukEF Independent [PDF]

Journal of Biological Chemistry, 2016
Properly condensed chromosomes are necessary for accurate segregation of the sisters after DNA replication. The Escherichia coli condesin is MukB, a structural maintenance of chromosomes (SMC)-like protein, which forms a complex with MukE and the kleisin MukF. MukB is known to be able to mediate knotting of a DNA ring, an intramolecular reaction.
Soon, Bahng, Ryo, Hayama, Kenneth J, Marians   +2 more
openaire   +2 more sources

Mutants Suppressing Novobiocin Hypersensitivity of amukBNull Mutation [PDF]

Journal of Bacteriology, 2003
ABSTRACTThemukBgene is essential for the partitioning of sister chromosomes inEscherichia coli. AmukBnull mutant is hypersensitive to the DNA gyrase inhibitor novobiocin. In this work, we isolated mutants suppressing the novobiocin hypersensitivity of themukBnull mutation.
Shun, Adachi, Sota, Hiraga
openaire   +2 more sources

The MukB–topoisomerase IV interaction is required for proper chromosome compaction [PDF]

Journal of Biological Chemistry, 2017
The bacterial condensin MukB and the cellular decatenating enzyme topoisomerase IV interact. This interaction stimulates intramolecular reactions catalyzed by topoisomerase IV, supercoiled DNA relaxation, and DNA knotting but not intermolecular reactions such as decatenation of linked DNAs.
Rupesh, Kumar, Pearl, Nurse, Soon, Bahng, Chong M, Lee, Kenneth J, Marians   +4 more
openaire   +2 more sources

Partition of P1 plasmids in Escherichia coli mukB chromosomal partition mutants [PDF]

Journal of Bacteriology, 1995
The partition system of the low-copy-number plasmid/prophage of bacteriophage P1 encodes two proteins, ParA and ParB, and contains a DNA site called parS. ParB and the Escherichia coli protein IHF bind to parS to form the partition complex, in which parS is wrapped around ParB and IHF in a precise three-dimensional conformation.
B E, Funnell, L, Gagnier
openaire   +2 more sources