Results 21 to 30 of about 829 (144)
MukB-mediated Catenation of DNA Is ATP and MukEF Independent [PDF]
Journal of Biological Chemistry, 2016 Properly condensed chromosomes are necessary for accurate segregation of the sisters after DNA replication. The Escherichia coli condesin is MukB, a structural maintenance of chromosomes (SMC)-like protein, which forms a complex with MukE and the kleisin MukF. MukB is known to be able to mediate knotting of a DNA ring, an intramolecular reaction.
Soon, Bahng +2 more
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Competitive binding of MatP and topoisomerase IV to the MukB dimerization hinge [PDF]
, 2021 ABSTRACT SMC complexes have ubiquitous roles in chromosome organisation. In Escherichia coli, the interplay between the SMC complex, MukBEF, and matS-bound MatP in the replication termination region, ter, results in depletion of MukBEF from ter, thus promoting chromosome ...
Gemma L. M. Fisher +10 more
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A topological analysis of difference topology experiments of condensin with topoisomerase II
Biology Open, 2020 An experimental technique called difference topology combined with the mathematics of tangle analysis has been used to unveil the structure of DNA bound by the Mu transpososome. However, difference topology experiments can be difficult and time consuming.
Soojeong Kim, Isabel K. Darcy
doaj +1 more source
Molecular Microbiology, Volume 114, Issue 5, Page 839-856, November 2020., 2020 The rod‐shaped Myxococcus xanthus cells have a unique chromosome organization whereby the chromosome occupies the central part of cells leaving large subpolar regions devoid of DNA. We show that three systems contribute to chromosome segregation and organization in M.
Deepak Anand +2 more
wiley +1 more source
Mutants Suppressing Novobiocin Hypersensitivity of amukBNull Mutation [PDF]
Journal of Bacteriology, 2003 ABSTRACTThemukBgene is essential for the partitioning of sister chromosomes inEscherichia coli. AmukBnull mutant is hypersensitive to the DNA gyrase inhibitor novobiocin. In this work, we isolated mutants suppressing the novobiocin hypersensitivity of themukBnull mutation.
Shun, Adachi, Sota, Hiraga
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Spo0J and SMC are required for normal chromosome segregation in Staphylococcus aureus
MicrobiologyOpen, Volume 9, Issue 4, April 2020., 2020 Chromosome segregation is an essential but poorly understood process in spherical bacteria such as Staphylococcus aureus. Here, we show that the staphylococcal ParB homologue, Spo0J, and the structural maintenance of chromosomes (SMC) protein act in concert to achieve efficient chromosome segregation in S. aureus. We demonstrate that Spo0J and SMC form
Helena Chan +2 more
wiley +1 more source
The MukB-ParC Interaction Affects the Intramolecular, Not Intermolecular, Activities of Topoisomerase IV [PDF]
Journal of Biological Chemistry, 2013 Proper chromosome organization is accomplished through binding of proteins such as condensins that shape the DNA and by modulation of chromosome topology by the action of topoisomerases. We found that the interaction between MukB, the bacterial condensin, and ParC, a subunit of topoisomerase IV, enhanced relaxation of negatively supercoiled DNA and ...
Ryo, Hayama +3 more
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MukBs cooperate to loop DNA [PDF]
The Journal of Cell Biology, 2008 When it comes to corralling DNA, MukB is the little condensin that could, say Yuanbo Cui, Aoya Petrushenko, and Valentin Rybenkov (University of Oklahoma, Norman, OK). Small in size but with a big effect, condensins are believed to stabilize large DNA loops, thereby giving order to an ...
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Partitioning of a mini-F plasmid into anucleate cells of the mukB null mutant [PDF]
Journal of Bacteriology, 1991 The partition-proficient mini-F plasmid pXX325 was stably maintained in the mukB null mutant, which is defective in chromosome partitioning into the two daughter cells. In the null mutant, the plasmid was partitioned into both nucleate and anucleate daughter cells, independently of host chromosomes.
B, Ezaki, T, Ogura, H, Niki, S, Hiraga
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Partition of P1 plasmids in Escherichia coli mukB chromosomal partition mutants [PDF]
Journal of Bacteriology, 1995 The partition system of the low-copy-number plasmid/prophage of bacteriophage P1 encodes two proteins, ParA and ParB, and contains a DNA site called parS. ParB and the Escherichia coli protein IHF bind to parS to form the partition complex, in which parS is wrapped around ParB and IHF in a precise three-dimensional conformation.
B E, Funnell, L, Gagnier
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