The Crystal Structure of the Hinge Domain of the Escherichia coli Structural Maintenance of Chromosomes Protein MukB [PDF]
MukB, a divergent structural maintenance of chromosomes (SMC) protein, is important for chromosomal segregation and condensation in gamma-proteobacteria. MukB and canonical SMC proteins share a characteristic five-domain structure. Globular N- and C-terminal domains interact to form an ATP-binding cassette-like ATPase or "head" domain, which is ...
Yinyin, Li +3 more
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Cell boundary confinement sets the size and position of the E. coli chromosome [PDF]
Although the spatiotemporal structure of the genome is crucial to its biological function, many basic questions remain unanswered on the morphology and segregation of chromosomes.
Chaudhuri, Debasish +11 more
core +2 more sources
Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli [PDF]
Escherichia coli endoribonuclease E has a major influence on gene expression. It is essential for the maturation of ribosomal and transfer RNA as well as the rapid degradation of messenger RNA.
Clarke, JE +3 more
core +1 more source
Different localization of SeqA‐bound nascent DNA clusters and MukF–MukE–MukB complex in Escherichia coli cells [PDF]
MukF, MukE and MukB proteins form a complex that may participate in the organization of folded sister chromosomes in Escherichia coli. We have found that a MukB–GFPuv4 fusion protein is observed as discrete fluorescent foci, which are localized within cellular spaces occupied by nucleoids, but not at the constriction site of cell division in living ...
K, Ohsumi, M, Yamazoe, S, Hiraga
openaire +2 more sources
Prospect for conservation agriculture in Northern and Eastern European countries, lessons of Kassa [PDF]
SYNOPSIS. In Europe, Conservation agriculture (CA) is less adopted than in the other world adopting regions and, reduced tillage is more used than no-tillage. Use of cover crop is not common.
Barz, P. +9 more
core
In vitro topological loading of bacterial condensin MukB on DNA, preferentially single-stranded DNA rather than double-stranded DNA [PDF]
AbstractCondensin is the major driving force in the segregation of daughter chromosomes in prokaryotes. Core subunits of condensin belong to the SMC protein family, whose members are characterized by a unique ATPase activity and dimers with a V-shaped structure.
Hironori Niki, Koichi Yano
openaire +2 more sources
Adjacent single-stranded regions mediate processing of tRNA precursors by RNase E direct entry [PDF]
The RNase E family is renowned for being central to the processing and decay of all types of RNA in many species of bacteria, as well as providing the first examples of endonucleases that can recognize 50 -monophosphorylated ends thereby increasing
Andrade +67 more
core +2 more sources
Disrupting the Acyl Carrier Protein/SpoT Interaction In Vivo: Identification of ACP Residues Involved in the Interaction and Consequence on Growth [PDF]
In bacteria, Acyl Carrier Protein (ACP) is the central cofactor for fatty acid biosynthesis. It carries the acyl chain in elongation and must therefore interact successively with all the enzymes of this pathway.
A Battesti +49 more
core +6 more sources
Multiple activities of the MatP protein are involved in post-replicative pairing of sister chromosomes in Escherichia coli [PDF]
The ter region of the bacterial chromosome, where replication terminates, is the last to be segregated before cell division in Escherichia coli. Delayed segregation is controlled by the MatP protein, which binds to specific sites (matS) within ter, and ...
Bertoni, Tom-Maso +9 more
core +3 more sources
Gene Transcription and Chromosome Replication in Escherichia coli [PDF]
Transcript levels of several Escherichia coli genes involved in chromosome replication and cell division were measured in dnaC2(Ts) mutants synchronized for chromosome replication by temperature shifts.
Bogan, Joseph A. +6 more
core +2 more sources

