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Optimized efflux assay for the NorA multidrug efflux pump in Staphylococcus aureus
Journal of Microbiological Methods, 2017Real-time fluorescent efflux assays are commonly used for measuring the efflux of bacterial pumps. Here we describe an optimized protocol for the NorA efflux pump in S. aureus using DiOC3 instead of ethidium bromide. Glucose and sodium formate were tested as energy carriers. This novel method is fast and reproducible.
Saskia, Zimmermann +4 more
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2016
Plant pathogens belonging to the genus Erwinia cause diseases in several economically important plants. Plants respond to bacterial infection with a powerful chemical arsenal and signaling molecules to rid themselves of the microbes. Although our understanding of how Erwinia initiate infections in plants has become clear, a comprehensive understanding ...
J, Thekkiniath +2 more
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Plant pathogens belonging to the genus Erwinia cause diseases in several economically important plants. Plants respond to bacterial infection with a powerful chemical arsenal and signaling molecules to rid themselves of the microbes. Although our understanding of how Erwinia initiate infections in plants has become clear, a comprehensive understanding ...
J, Thekkiniath +2 more
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Small Multidrug Resistance Efflux Pumps
2016Small multidrug resistance (SMR) transporters confer resistance to a variety of quaternary cation compound antimicrobials. These secondary active transporters are the smallest known transporters and have been demonstrated to function within the membrane.
Denice C. Bay, Raymond J. Turner
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Assembly and operation of bacterial tripartite multidrug efflux pumps
Trends in Microbiology, 2015Microorganisms encode several classes of transmembrane pumps that can expel an enormous range of toxic substances, thereby improving their fitness in harsh environments and contributing to resistance against antimicrobial agents. In Gram-negative bacteria these pumps can take the form of tripartite assemblies that actively efflux drugs and other ...
Dijun, Du +2 more
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Multidrug Efflux Pumps Attenuate the Effect of MGMT Inhibitors
Molecular Pharmaceutics, 2015Various mechanisms of drug resistance attenuate the effectiveness of cancer therapeutics, including drug transport and DNA repair. The DNA repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) is a key factor determining the resistance against alkylating anticancer drugs inducing the genotoxic DNA lesions O(6)-methylguanine and O(6 ...
Karl-Heinz, Tomaszowski +2 more
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Antibiotics: Pharmacokinetics, toxicity, resistance and multidrug efflux pumps
Biochemical Pharmacology, 2017The discovery of penicillin followed by streptomycin, tetracycline, cephalosporins and other natural, semi-synthetic and synthetic antimicrobials completely revolutionized medicine by reducing human morbidity and mortality from most of the common infections. However, shortly after they were introduced to clinical practice, the development of resistance
Çiğdem, Yılmaz, Gülay, Özcengiz
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Chloramphenicol and expression of multidrug efflux pump in Enterobacter aerogenes
Biochemical and Biophysical Research Communications, 2005Chloramphenicol has been reported to act as an inducer of the multidrug resistance in Escherichia coli. A resistant variant able to grow on plates containing 64 microg/ml chloramphenicol was obtained from the Enterobacter aerogenes ATCC 13048-type strain.
Didier, Ghisalberti +3 more
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Imipenem and expression of multidrug efflux pump in Enterobacter aerogenes
Biochemical and Biophysical Research Communications, 2003Imipenem is often used to treat intensive care unit patients infected by Enterobacter aerogenes, but it is leading to an increasing number of antibiotic resistant strains. Clinical isolates and imipenem resistant variants presented a high level of resistance to beta-lactam antibiotic group and to chemically unrelated drugs. We report here that imipenem
Charléric, Bornet +6 more
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Role of bacterial multidrug efflux pumps during infection
World Journal of Microbiology and BiotechnologyMultidrug efflux pumps are protein complexes located in the cell envelope that enable bacteria to expel, not only antibiotics, but also a wide array of molecules relevant for infection. Hence, they are important players in microbial pathogenesis. On the one hand, efflux pumps can extrude exogenous compounds, including host-produced antimicrobial ...
Pablo Laborda +4 more
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Multidrug efflux pumps in Staphylococcus aureus and their clinical implications
Journal of Microbiology, 2016Antibiotic resistance is rapidly spreading among bacteria such as Staphylococcus aureus, an opportunistic bacterial pathogen that causes a variety of diseases in humans. For the last two decades, bacterial multidrug efflux pumps have drawn attention due to their potential association with clinical multidrug resistance.
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