Results 1 to 10 of about 281,013 (338)

Multiplex Detection of Aspergillus fumigatus Mycoviruses [PDF]

Viruses, 2018
Mycoviruses are viruses that naturally infect and replicate in fungi. They are widespread in all major fungal groups including plant and animal pathogenic fungi. Several dsRNA mycoviruses have been reported in Aspergillus fumigatus.
Selin Özkan-Kotiloğlu, Robert H. A. Coutts   +1 more
doaj   +5 more sources

Diferenciação de micobactérias por PCR multiplex Differentiation of micobacteria by multiplex PCR

Revista da Sociedade Brasileira de Medicina Tropical, 2009
O trabalho visou à otimização de um método baseado na reação em cadeia da polimerase multiplex - para diferenciação de micobactérias de interesse para a saúde pública.
Diogo da Rocha Poroca, Andrea Santos Lima, Juliana Falcão de Araújo Lima, Heidi Lacerda Alves da Cruz, Rosana de Albuquerque Montenegro, Fábio Lopes de Melo, Haiana Charifker Schindler, Lílian Maria Lapa Montenegro   +7 more
doaj   +2 more sources

A novel XbaI multiplex PCR method for rapid typing of Klebsiella pneumoniae strains [PDF]

Scientific Reports
This study focused on exploiting single-nucleotide polymorphism (SNP) variations in and around XbaI-restriction sites (5′….T↓CTAGA….3′) within the genomes of Klebsiella pneumoniae to develop a multiplex PCR genotyping technique that integrates the high ...
Ibrahim Ali Al-Zahrani
doaj   +2 more sources

Identification of novel clostridium perfringens type E strains that carry an iota toxin plasmid with a functional enterotoxin gene [PDF]

, 2011
Clostridium perfringens enterotoxin (CPE) is a major virulence factor for human gastrointestinal diseases, such as food poisoning and antibiotic associated diarrhea. The CPE-encoding gene (cpe) can be chromosomal or plasmid-borne.
A Deguchi, A Gurjar, A Heikinheimo, BA McClane, Bruce A. McClane, D Lepp, Herman Tse, HS Garmony, J Li, J Li, J Li, J Li, J Li, JA Parsons, Jihong Li, K Miyamoto, K Miyamoto, K Miyamoto, K Miyamoto, Kanako Mimura, Kazuaki Miyamoto, KH Harry, Masahiro Nagahama, MR Sarker, Natsuko Yumine, S Sayeed, S Sayeed, Shigeru Akimoto, SJ Billington, SR Mueller-Spits, T Hirokawa, Y Miki, Y Zhao   +32 more
core   +18 more sources

Outcomes and clinical relevance of stool multiplex bacterial polymerase chain reaction in patients with acute diarrhea: single center experience [PDF]

The Korean Journal of Internal Medicine, 2020
Background/Aims Diagnostic stool multiplex polymerase chain reaction (PCR) testing has attracted considerable interest, because of its high sensitivity, short turnaround time, and ability to detect multiple organisms simultaneously.
Won Gun Kwack, Yun Jeong Lim, Ki Hwan Kwon, Jae Woo Chung, Jin Young Oh   +4 more
doaj   +1 more source

Usefulness of Stool Multiplex Polymerase Chain Reaction Assays in Patients with Acute Diarrhea

The Korean Journal of Gastroenterology, 2022
Background/Aims: There is a recent increase in the use of stool multiplex PCR assay-based diagnostic tests in patients with acute diarrhea. We used multiplex PCR assays to analyze the distribution of diarrhea-causing bacteria and viruses, as well as the ...
Seo Hyun Kim, You Sun Kim, Seung Hyuk Kim, Won Eui Yoon, Hee Jun Myung, Jeong Seop Moon, Dong Hee Whang   +6 more
doaj   +1 more source

Diagnostic accuracy of multiplex polymerase chain reaction on tissue biopsies in periprosthetic joint infections

Scientific Reports, 2021
The diagnosis and treatment of periprosthetic joint infection (PJI) currently relies on cultures, which are time-consuming and often fail. Multiplex PCR assays promise reliable and prompt results, but have been heterogeneously evaluated.
Igor Lazic, Susanne Feihl, Peter M. Prodinger, Ingo J. Banke, Andrej Trampuz, Rüdiger von Eisenhart-Rothe, Christian Suren   +6 more
doaj   +1 more source

Establishment of a Multiplex RT-PCR Method for the Detection of Five Known Genotypes of Porcine Astroviruses

Frontiers in Veterinary Science, 2021
Porcine astroviruses (PAstVs) are prevalent in pigs worldwide, and five genotypes have been reported to circulate in China. However, little is known about the coinfection status of PAstVs.
Xin Liu, Wenchao Zhang, Dongjing Wang, Xinyue Zhu, Ying Chen, Kang Ouyang, Zuzhang Wei, Huan Liu, Weijian Huang   +8 more
doaj   +1 more source

Selecting a multiplex PCR panel for accurate molecular diagnosis of intestinal protists: a comparative study of Allplex® (Seegene®), G-DiaParaTrio (Diagenode®), and RIDA®GENE (R-Biopharm®) assays and microscopic examination

Parasite, 2022
Commercial multiplex PCR assay panels were developed to overcome the limitations of microscopic examination for parasitological diagnosis on stool samples. However, given the increased supply of this diagnostic approach, these assays must be evaluated to
Argy Nicolas, Nourrisson Céline, Aboubacar Ahmed, Poirier Philippe, Valot Stéphane, Laude Adrien, Desoubeaux Guillaume, Pomares Christelle, Machouart Marie, Le Govic Yohann, Dalle Frédéric, Botterel Françoise, Bourgeois Nathalie, Cateau Estelle, Leterrier Marion, Le Pape Patrice, Morio Florent, Houze Sandrine   +17 more
doaj   +1 more source

Detection and identification of mycobacteria in sputum from suspected tuberculosis patients

BMC Research Notes, 2010
Background Detection of Tuberculosis agent like nontuberculous mycobacteria (NTM) species by culture and microscopic methods remains difficult and time consuming.
Yadi, Masjudi, Tandirogang Nataniel, Sultan Andi, Hatta Mochammad   +4 more
doaj   +1 more source