Evaluation of a Multiplex PCR Assay for the Identification of Campylobacter jejuni and Campylobacter coli [PDF]
, 2017 Background: Campylobacter jejuni and Campylobacter coli are identified as the major causes of acute gastroenteritis in humans. Because of the fastidious nature of Campylobacters, many clinical laboratories fail to routinely culture them.
Ghorbanalizadgan, Mehdi +3 more
core +1 more source
PlantID – DNA-based identification of multiple medicinal plants in complex mixtures [PDF]
, 2012 Background An efficient method for the identification of medicinal plant products is now a priority as the global demand increases. This study aims to develop a DNA-based method for the identification and authentication of plant species that can be ...
Bremner, Paul +7 more
core +2 more sources
Clinical use of multiplex-PCR for the diagnosis of acute bacterial meningitis
Journal of Family Medicine and Primary Care, 2022 Background and Objectives: Prompt and accurate diagnosis of acute bacterial meningitis (ABM) is critical for patient management. We designed and evaluated two sets of multiplex-PCR assays for the simultaneous detection of six major etiologies of ABM i.e.,
Nupur Sharma +8 more
doaj +1 more source
PCR for the detection of pathogens in neonatal early onset sepsis. [PDF]
, 2020 BACKGROUND: A large proportion of neonates are treated for presumed bacterial sepsis with broad spectrum antibiotics even though their blood cultures subsequently show no growth. This study aimed to investigate PCR-based methods to identify pathogens not
Bedford Russell, A +8 more
core +1 more source
Iranian Journal of Microbiology, 2020 Background and Objectives: Acinetobacter baumannii has been known as a major pathogen causing nosocomial infections. The aim of this study was to develop multiplex PCR for rapid and simultaneous detection of metallo-β-lactamase (MBL) genes in clinical ...
Reza Ranjbar +2 more
doaj +1 more source
Species-specific Real Time-PCR primers/probe systems to identify fish parasites of the genera Anisakis, Pseudoterranova and Hysterothylacium (Nematoda: Ascaridoidea) [PDF]
, 2018 Ascaridoid nematodes belonging to the genera Anisakis and Pseudoterranova are heteroxenous parasites, involving marine mammals as definitive hosts in their life-cycles, whereas crustaceans (krill), fish and squids acting as intermediate/paratenic hosts.
Colantoni, Alessandra +5 more
core +1 more source
Regional Differences in Presence of Shiga toxin-producing Escherichia coli Virulence-Associated Genes in the Environment in the North West and East Anglian regions of England [PDF]
, 2020 Shiga toxin-producing Escherichia coli is carried in the intestine of ruminant animals, and outbreaks have occurred after contact with ruminant animals or their environment.
Hunter, Paul R +6 more
core +1 more source
Multiplex PCR: Critical Parameters and Step-by-Step Protocol
BioTechniques, 1997 By simultaneously amplifying more than one locus in the same reaction, multiplex PCR is becoming a rapid and convenient screening assay in both the clinical and the research laboratory.
O. Henegariu +4 more
doaj +1 more source
Animals, 2023 Porcine reproductive and respiratory syndrome virus (PRRSV) type 1 (European genotype) and PRRSV type 2 (North American genotype) are prevalent all over the world.
Feng Long +5 more
doaj +1 more source
Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection. [PDF]
, 2019 BACKGROUND:Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants
A Bak +35 more
core +1 more source