Two-color quantitative multiplex methylation-specific PCR [PDF]
BioTechniques, 2006 In recent years, several methylation-specific PCR-based techniques have been developed to identify and characterize hypermethylation of CpG dinucleotides with the primary goal of elucidating a better understanding of the role of DNA methylation in ...
Theresa Swift-Scanlan +4 more
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Evaluation of a Multiplex PCR Assay for the Identification of Campylobacter jejuni and Campylobacter coli [PDF]
, 2017 Background: Campylobacter jejuni and Campylobacter coli are identified as the major causes of acute gastroenteritis in humans. Because of the fastidious nature of Campylobacters, many clinical laboratories fail to routinely culture them.
Ghorbanalizadgan, Mehdi +3 more
core +1 more source
Clinical use of multiplex-PCR for the diagnosis of acute bacterial meningitis
Journal of Family Medicine and Primary Care, 2022 Background and Objectives: Prompt and accurate diagnosis of acute bacterial meningitis (ABM) is critical for patient management. We designed and evaluated two sets of multiplex-PCR assays for the simultaneous detection of six major etiologies of ABM i.e.,
Nupur Sharma +8 more
doaj +1 more source
PlantID – DNA-based identification of multiple medicinal plants in complex mixtures [PDF]
, 2012 Background An efficient method for the identification of medicinal plant products is now a priority as the global demand increases. This study aims to develop a DNA-based method for the identification and authentication of plant species that can be ...
Bremner, Paul +7 more
core +2 more sources
The Pan African Medical Journal, 2016 This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo.
Maleki Assih +6 more
doaj +1 more source
Iranian Journal of Microbiology, 2020 Background and Objectives: Acinetobacter baumannii has been known as a major pathogen causing nosocomial infections. The aim of this study was to develop multiplex PCR for rapid and simultaneous detection of metallo-β-lactamase (MBL) genes in clinical ...
Reza Ranjbar +2 more
doaj +1 more source
Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection. [PDF]
, 2019 BACKGROUND:Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants
A Bak +35 more
core +1 more source
BioTechniques, 2000 By simultaneously amplifying several loci in the same reaction, multiplex PCR has been used in gene mapping and DNA typing with polymorphic short tandem repeat loci.
H.-W. Deng +4 more
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Species-specific Real Time-PCR primers/probe systems to identify fish parasites of the genera Anisakis, Pseudoterranova and Hysterothylacium (Nematoda: Ascaridoidea) [PDF]
, 2018 Ascaridoid nematodes belonging to the genera Anisakis and Pseudoterranova are heteroxenous parasites, involving marine mammals as definitive hosts in their life-cycles, whereas crustaceans (krill), fish and squids acting as intermediate/paratenic hosts.
Colantoni, Alessandra +5 more
core +1 more source
Multiplex PCR: Critical Parameters and Step-by-Step Protocol
BioTechniques, 1997 By simultaneously amplifying more than one locus in the same reaction, multiplex PCR is becoming a rapid and convenient screening assay in both the clinical and the research laboratory.
O. Henegariu +4 more
doaj +1 more source